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International Journal of Legal Medicine

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01.11.2005 | Technical Note

Microsatellite genotyping of DNA isolated from claws left on tanned carnivore hides

verfasst von: Eva Hedmark, Hans Ellegren

Erschienen in: International Journal of Legal Medicine | Ausgabe 6/2005

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Abstract

Tanned hides, a common form of preservation of mammalian specimens, are usually resistant to DNA analysis. However, we show that DNA isolated from the pulp of claws of tanned hides amplifies well for microsatellite markers. For eight wolverine and eight lynx hides tanned 5–20 years ago, 93–98% of replicate amplifications gave distinct PCR products. Genotypes obtained in analysis of tissue samples of the same individuals were in all cases in agreement with those obtained by analysis of claws. We thus conclude that the use of claws from tanned hides offers new possibilities to genetic studies of preserved mammalian specimens, for instance, in the monitoring of illegal trade.

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Titel: Microsatellite genotyping of DNA isolated from claws left on tanned carnivore hides
verfasst von: Eva Hedmark
Hans Ellegren
Publikationsdatum: 01.11.2005
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 6/2005
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-005-0521-4

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Microsatellite genotyping of DNA isolated from claws left on tanned carnivore hides

Abstract

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