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26.09.2022 | Original Article

Photobiomodulation effects in metalloproteinases expression in zymosan-induced arthritis

verfasst von: Lucia Mara Januário dos Anjos, Anna Cecília Quirino-Teixeira, Eugenio Damasceno Hottz, Adenilson de Souza da Fonseca, Jacy Gameiro, Flávia de Paoli

Erschienen in: Lasers in Medical Science | Ausgabe 9/2022

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Abstract

Matrix metalloproteinases (MMPs) play a crucial role in the degenerative course of rheumatic disorders. They are responsible for cartilage and other joint-associated tissues breakdown. Amid arthritis treatments, photobiostimulation (PBM), a non-thermal and non-invasive low-power laser application, appears to be an outstanding therapy alternative once it has succeeded in MMPs modulation. Thus, this study aimed to evaluate the PBM effects of low infrared laser (830 nm), testing two different energy densities (3 and 30 Jcm⁻²) in MMP-2, MMP-9, MMP-13, and MMP-14 as well as the inhibitor TIMP-2 expressions using zymosan-induced arthritis model. C57BL/6 mice were distributed into four groups (n = 8): zymosan-induced arthritis without treatment; zymosan-induced arthritis and dexamethasone-treated; zymosan-induced arthritis and PBM at energy density of 3 Jcm⁻² treated; and zymosan-induced arthritis and PBM at energy density of 30 Jcm⁻² treated. MMPs and TIMP-2 mRNA relative levels by qRT-PCR and proteins expression by immunohistochemical and Western blotting techniques were performed after PBM treatment in the inflamed joint. Our results demonstrated PBM could modulate both mRNA relative levels and proteins expression of the MMP-2, -9, -13, -14, and TIMP-2 in joint tissues, decreasing MMP-9 protein expression and increasing TIMP-2 protein expression. PBM promotes a better arthritis prognostic, modulating metalloproteinase and its inhibitor, especially MMP-9 and TIMP-2 protein expression that is important inflammatory markers. These findings may also corroborate that PBM may regulate MMPs expression using different pathways.

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Titel: Photobiomodulation effects in metalloproteinases expression in zymosan-induced arthritis
verfasst von: Lucia Mara Januário dos Anjos
Anna Cecília Quirino-Teixeira
Eugenio Damasceno Hottz
Adenilson de Souza da Fonseca
Jacy Gameiro
Flávia de Paoli
Publikationsdatum: 26.09.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03647-4

Springer Medizin

Abstract

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