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Pediatric Surgery International

Erschienen in:

01.12.2023 | Original Article

Polyphyllin D induces necroptosis in neuroblastoma cells (IMR-32 and LA-N-2) in mice

verfasst von: Shunsuke Watanabe, Mikihiro Inoue, Tatsuya Suzuki, Yasuhiro Kondo, Mika Murayama

Erschienen in: Pediatric Surgery International | Ausgabe 1/2023

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Abstract

Background

We previously reported that polyphyllin D, the main component of the traditional herbal medicinal Paris polyphylla, exhibited anticancer effects in vitro against human neuroblastoma cells. The aim of this investigation was to examine in vivo antitumor effects of polyphyllin D.

Methods

Subcutaneous tumors were established in immune-deficient BALB/c nude mice using human neuroblastoma cell lines IMR-32 and LA-N-2. To evaluate the polyphyllin D activity, we used a mouse model of IMR-32 or LA-N-2 cell lines and analyzed subcutaneous tumors.

Results

Subcutaneous tumor models were successfully established in mice using two human neuroblastoma cell lines. In the subcutaneous tumor model, porphyrin D was found to suppress tumor volume. We found that polyphyllin D suppressed the number of foci by Ki-67 staining (IMR-32 and LA-N-2; p < 0.01, 0.02, respectively). We found that polyphyllin D induces the RIPK3 expression, while polyphyllin D phosphorylates Ser358 in IMR-32 and Ser358 and Tyr376 in LA-N-2.

Conclusion

We developed a mouse model of subcutaneous tumors of neuroblastoma and demonstrated for the first time that polyphyllin D has an antitumor effect on neuroblastoma. Polyphyllin D can cause necroptosis depending on the cell type. The new drug can be expected by investigating a method to selectively induce cell death through the analysis of necroptosis.

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Titel: Polyphyllin D induces necroptosis in neuroblastoma cells (IMR-32 and LA-N-2) in mice
verfasst von: Shunsuke Watanabe
Mikihiro Inoue
Tatsuya Suzuki
Yasuhiro Kondo
Mika Murayama
Publikationsdatum: 01.12.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 1/2023
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-023-05425-x

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Polyphyllin D induces necroptosis in neuroblastoma cells (IMR-32 and LA-N-2) in mice

Abstract

Background

Methods

Results

Conclusion

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Abstract

Background

Methods

Results

Conclusion

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