Erschienen in:
01.10.2012 | Original Research Paper
Pro-inflammatory cytokine interleukin-1β promotes the development of intestinal stem cells
verfasst von:
Lei Wang, Ziyan Liu, Yijing Li, Loretta Pappan, Amy Galliher-Beckley, Jishu Shi
Erschienen in:
Inflammation Research
|
Ausgabe 10/2012
Einloggen, um Zugang zu erhalten
Abstract
Objective
We investigated the effect of IL-1β on the development of intestinal epithelial stem cells.
Materials and methods
Normal intestinal epithelial cell line IEC-18 cells were cultured in the presence or absence of 200 pM of IL-1β in serum-free medium (SFM) for various time periods. The effects of IL-1β on intestinal stem cell self-renewal and IEC-18 cell proliferation were evaluated by a colony formation assay, MTT assay, and a focus formation assay. The expression of stemness genes including Bmi-1, Lgr-5, c-myc, Nanog, and β-catenin in IEC-18 cells were measured by quantitative PCR and western blot analysis.
Results
IEC-18 cells grew as a monolayer in SFM in the absence of IL-1β. Cellular spheres were formed when IEC-18 cells were grown in SFM in the presence of IL-1β. IL-1β induced the development of large colonies in the soft-agar as well as the formation of foci when IEC-18 cells were cultured in type-I collagen-coated plates. The expression of Bmi-1, Lgr-5, c-myc, Nanog, and β-catenin were significantly increased in IEC-18 cells treated with IL-1β.
Conclusion
Our studies provide direct evidence the IL-1β may play an important role in the self-renewal of intestinal epithelial stem cells and the development of cancer stem cells.