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01.11.2012 | Reproductive Medicine

Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection

verfasst von: Mina Sharbatoghli, Mojtaba Rezazadeh Valojerdi, Massoud Amanlou, Fariba Khosravi, Mohammad Asghari Jafar-abadi

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 5/2012

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Abstract

Purpose

The objective of this study was to assess the relationship of DNA damage, apoptosis and dysfunction of mitochondrial membrane potential (MMP) in ejaculated spermatozoa with semen parameters (sperm concentration, motility and normal morphology) and to evaluate their effects on assisted reproductive technology (ART) outcomes after intracytoplasmic sperm injection (ICSI).

Methods

Semen parameters in 120 infertile couples who underwent ICSI treatment were routinely analyzed and examined for the incidence of sperm DNA fragmentation (DF) by the sperm chromatin dispersion test (SCD). Whereas the incidences of sperm apoptosis and dysfunction of MMP were assessed by flow cytometry. The correlation among different sperm factors and ART outcomes was evaluated statistically.

Results

Sperm parameters were negatively related to DF (motility and normal morphology, p < 0.01), apoptosis (concentration, motility and normal morphology, p < 0.01, p < 0.05 and p < 0.05, p < 0.01 respectively), and dysfunction of sperm MMP (concentration, motility and normal morphology, p < 0.01). DF also showed a positive correlation with apoptosis and dysfunction of sperm MMP (p < 0.05, and p < 0.01 respectively). However, there was no significant correlation among DF, apoptosis and dysfunction of sperm MMP with ART outcomes, except early apoptosis which showed significant (p < 0.05) negative correlation with pregnancy rate.

Conclusion

In the present study; DF, apoptosis and dysfunction of sperm MMP indicated negative relationship with sperm parameters. Although there was a negative correlation between early apoptosis and pregnancy rate, no significant correlation was observed between these parameters and ICSI outcomes.

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Titel: Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection
verfasst von: Mina Sharbatoghli
Mojtaba Rezazadeh Valojerdi
Massoud Amanlou
Fariba Khosravi
Mohammad Asghari Jafar-abadi
Publikationsdatum: 01.11.2012
Verlag: Springer-Verlag
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 5/2012
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2440-1

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Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection