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01.11.2010 | Original Article

The effects of eccentric contraction on myofibrillar proteins in rat skeletal muscle

verfasst von: Keita Kanzaki, Mai Kuratani, Takaaki Mishima, Satoshi Matsunaga, Noriyuki Yanaka, Sachio Usui, Masanobu Wada

Erschienen in: European Journal of Applied Physiology | Ausgabe 5/2010

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Abstract

The present study investigated the effects of eccentric muscle contractions (ECC) on the content of myofibrillar proteins (my-proteins) and the catalytic activity of myofibrillar ATPase (my-ATPase) in skeletal muscles. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC or isometric contractions (ISC) and used for measures of force output and for biochemical analyses, respectively. Whereas in ISC-treated muscles, full restoration of tetanic force was attained after 2 days of recovery, force developed by ECC-treated muscles remained depressed (P < 0.05) after 6 days. The total my-protein content and the relative content of myosin heavy chain (MHC) in total my-proteins were unaltered during 4 days of recovery after ECC, but fell (P < 0.05) to 55.9 and 63.4% after 6 days of recovery, respectively. my-ATPase activity expressed on a my-protein weight basis was unaltered immediately after ECC. However, it decreased (P < 0.05) to 75.3, 45.3, and 49.3% after 2, 4 and 6 days of recovery, respectively. Total maximal calpain activity measured at 5 mM Ca²⁺ was significantly augmented (P < 0.05) after 2 days of recovery, reaching a level of threefold higher after 6 days. These alterations were specific for ECC and not observed for ISC. These results suggest that depressions in my-ATPase activity contribute to ECC-induced decreases in force and power which can take a number of days to recover.

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Titel: The effects of eccentric contraction on myofibrillar proteins in rat skeletal muscle
verfasst von: Keita Kanzaki
Mai Kuratani
Takaaki Mishima
Satoshi Matsunaga
Noriyuki Yanaka
Sachio Usui
Masanobu Wada
Publikationsdatum: 01.11.2010
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 5/2010
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-010-1579-3

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