Introduction
Methods
Study design and patients
Bronchoscopy methods
Specimens collection
Extracted DNA and RNA and NGS analysis
Statistical analysis
Results
Patient characteristics
Frozen Cytology Pellet (n = 75) | Frozen Tissue (n = 57) | ORR (95%CI) | P value | |
---|---|---|---|---|
Age-Median [IQR] | 72 [65.5–76] | 71 [66–74] | 0.47 | |
male/female | 58/17 | 43/14 | 0.9 (0.37–2.21) | 0.84 |
Brinkman Index Median [IQR] | 900 [440–1210] | 940 [570–1060] | 0.69 | |
primary tumor size(mm) Median [IQR] | 35 [25–46] | 50 [35–63] | < 0.01 | |
Stage | 0.08 | |||
II | 3 (4.0%) | 9 (15.8%) | ||
III | 32 (42.7%) | 17 (29.8%) | ||
IV | 38 (50.6%) | 29 (50.9%) | ||
Recurrent | 2 (2.7%) | 2 (3.5%) | ||
Histology | 0.37 | |||
Adeno | 39 (52.0%) | 27 (47.4%) | ||
Sq | 17 (22.6%) | 21 (36.8%) | ||
NOS | 9 (12.0%) | 4 (7.0%) | ||
SCLC | 9 (12.0%) | 5 (8.8%) | ||
LCNEC | 1 (1.4%) | 0 | ||
Procedure | < 0.01 | |||
EBUS-TBNA | 31 (41.3%) | 3 (5.3%) | ||
TBB (ultrathin) | 1 (1.4%) | 1 (1.7%) | ||
EBUS-GS-TBB (thin) | 27 (36.0%) | 21 (36.8%) | ||
EBUS-GS-TBB (thick) | 4 (5.3%) | 11 (19.3%) | ||
EBB | 10 (13.3%) | 18 (31.6%) | ||
TBB under X-ray fluoroscopy | 2 (2.7%) | 3 (5.3%) |
Amounts of extracted DNA and RNA of frozen cytology pellets versus tissues
Frozen Cytology Pellet (n = 75) | Frozen Tissue (n = 57) | ORR (95%CI) | P value | |
Extracted amount (ng/mL)Median [IQR] | ||||
DNA | 60 [2–100] | 40 [3–70] | 0.34 | |
RNA | 10 [9.5–30] | 10 [5–29] | 0.4 | |
NGS success rate (%) | ||||
DNA sequencing | 97.3% | 98.2% | 1.5 (0.08–90.45) | 0.99 |
RNA sequencing | 76.0% | 77.2% | 1.16 (0.47–2.93) | 0.99 |
Frozen Cytology Pellet (n = 31) | Frozen Tissue (n = 3) | P value | ||
EBUS-TBNA NGS success rate (%) | ||||
DNA sequencing | 100.0% | 100.0% | ||
RNA sequencing | 83.9% | 33.3% | 0.11 (0.002–2.4) | 0.1 |
Analysis of NGS success rate
Univariate | |||
---|---|---|---|
ORR (95%CI) | P-value | ||
Factor | n= 75 | ||
Age | |||
≥ 75 | 28 (37.3%) | 1 | |
< 74 | 47 (62.7%) | 2.516 (0.676–11.82) | 0.167 |
Sex | |||
Male | 58 (77.3%) | 1 | |
Female | 17 (22.8%) | 1.435 (0.333–11.54) | 0.536 |
Histology | |||
Adeno | 39 (52.0%) | 1 | |
Non-adeno | 36 (48.0%) | 0.618 (0.176–2.043) | 0.427 |
Procedure | |||
EBUS-TBNA | 31 (41.3%) | 1 | |
TBB | 44 (58.7%) | 2.159 (0.618–8.791) | 0.272 |
Pellet specimens by EBUS-TBNA | n = 31 | ||
Bronchoscope | |||
UC290 | 15 (48.4%) | 1 | |
UC260FW | 16 (51.6%) | 1.481 (0.144–20.52) | 1 |
A number of puncture | |||
≥ 4 | 8 (25.8%) | 1 | |
< 3 | 23 (74.2%) | 0.463 (0.042–6.761) | 0.583 |