Afatinib plus osimertinib in the treatment of osimertinib-resistant non-small cell lung carcinoma: a phase I clinical trial

Molecularly targeted therapies have emerged as essential treatment modalities for non-small cell lung cancer (NSCLC). Somatic mutations in the epidermal growth factor receptor (EGFR) tyrosine kinase domain have been reported as definitive biomarkers for predicting the efficacy of EGFR-tyrosine kinase inhibitors (EGFR-TKIs) [1]. EGFR-TKI is the standard initial treatment option for patients with active EGFR mutations. Notably, in the FLAURA trial, osimertinib demonstrated superior efficacy to gefitinib or erlotinib for the initial treatment of patients with NSCLC [2]. Acquired resistance to initial osimertinib therapy remains the primary challenge for treating patients with EGFR mutation-positive NSCLC.

The mechanisms underlying osimertinib resistance can be divided into EGFR-dependent and -independent mechanisms [3]. The EGFR-independent mechanisms include activating alternative bypass-signaling pathways (such as in MET proto-oncogene receptor tyrosine kinase [MET] and HER2), oncogene fusions, or histologic transformations. Combination therapy with driver-matched inhibitors (e.g., osimertinib plus MET inhibitors for MET amplification) has been investigated as an alternative treatment for patients with bypass-signaling resistance mechanisms [4]. The EGFR-dependent mechanisms involve various mutations. The secondary EGFR C797S mutation has been characterized as the most frequent resistance mutation in EGFR-dependent mechanisms, accounting for 10–26% of patients in the second-line population or 7% in the first-line population [5].

Interestingly, preclinical data suggest that treatment with a combination of osimertinib and first/second-generation EGFR-TKIs can overcome the acquired C797S mutation [6]. Based on these preclinical data, concurrent EGFR-TKI therapy has been investigated to overcome this resistance mechanism (NCT03122717, NCT03810807, and NCT03944772). Other co-existing EGFR mutations are potentially sensitive to second-generation EGFR-TKIs, especially afatinib [7]. Afatinib, an irreversible EGFR-TKI, shows broad-range antitumor activity against EGFR mutations including the uncommon ones [8] [9]. Therefore, we considered that adding afatinib to osimertinib might help overcome EGFR-dependent osimertinib resistance, including the secondary C797S mutation and other co-existing EGFR mutations. Based on this rationale, we conducted a phase I trial to investigate the safety and efficacy of osimertinib and afatinib combination therapy in patients with acquired resistance to osimertinib.

This is the first prospective phase I study to investigate the effectiveness of afatinib combined with 80 mg osimertinib. The MTD was defined as 30 mg afatinib combined with 80 mg osimertinib. Based on the toxicity profiles found during all study periods, 66.6% of the patients treated with the MTDs of afatinib and osimertinib experienced DLTs or DLT-equivalent toxicity, such as persistent Grade 2 diarrhea lasting 4 days or Grade 3 rash, respectively. Therefore, the RD for further study was defined as 20 mg afatinib combined with 80 mg osimertinib.

Data regarding the adequate dose of afatinib for concomitant use with 80 mg osimertinib are limited. One case report suggested that 20 mg afatinib with 80 mg osimertinib was well tolerated and that it reduced the frequency of the G724S clone, as assessed by the plasma cell-free DNA testing [10]. In our trial, the MTD and RD of afatinib combined with 80 mg of osimertinib were 30 mg and 20 mg, respectively. The most frequent drug-related toxicities were diarrhea (76.9%), skin rash (69.2%), anorexia (38.5%), and oral mucositis (38.5%). These toxicities were manageable, especially in the 20 mg dose cohort. Low-dose afatinib therapy has already been investigated in several studies. A prospective phase II study using low-dose afatinib monotherapy reported that its clinical efficacy was comparable to that of standard-dose afatinib, without severe toxicities, for EGFR mutation-positive NSCLC [11, 12]. Considering these prior reports, the combination of 20 mg afatinib and 80 mg osimertinib will be a reasonable option for further investigations.

Osimertinib is superior to first-generation EGFR-TKIs used in the first-line treatment of EGFR mutation-positive advanced NSCLC [2]. Despite the high response rate and long PFS, many patients acquire resistance to osimertinib. Understanding the mechanisms of acquired osimertinib resistance is a major area of research. Here, we performed gene-mutation analysis to investigate the target efficacy of combination treatment with afatinib and osimertinib via liquid biopsy analysis. The gene mutations associated with EGFR were detected in 77% of patients before treatment. Other genetic alterations, such as KRAS, NPAP1, and TP53 mutations, were detected in six patients. Liquid biopsy analysis with NGS may be feasible for detecting druggable mutations and may help in evaluating osimertinib resistance. MET amplification, EGFR C797S mutations, and histologic transformations have been reported as major resistance mechanisms during initial osimertinib therapy [13]. C797 provides the covalent binding site for osimertinib, and mutations in this site induce resistance by interfering with drug-protein binding [14]. Interestingly, prior-generation EGFR-TKIs, such as gefitinib and afatinib, demonstrated sensitivity to C797S mutations [15]. The results of this study suggest that combination treatment with osimertinib and gefitinib or afatinib is a reasonable strategy against the C797S and T790M variants of EGFR. In this study, mutation profiling was performed with plasma samples using NGS. The three patients harboring the T790M/cis-C797S mutation pair did not show treatment responses before initiating combination therapy. C797S serves as the binding site for irreversible EGFR inhibitors, including osimertinib. The C797S mutation is considered a drug-resistance mutation with respect to osimertinib [16]. The location of C797S in cis or trans may have important biological implications [15]. The location of both T790M and C797S in the same allele (cis) led to EGFR-TKI resistance in vitro. The three C797S mutations detected in this study were located in cis with respect to the T790M mutation. The efficacy of combination treatment with osimertinib and afatinib against T790M/trans-C797S mutations remains unclear.

Secondary EGFR mutations or co-existing uncommon EGFR mutations, such as L718Q, G724S, L792H/F/Y, F795C, and G796S/R, have been reported to partially contribute to osimertinib resistance [17]. Several reports have investigated the efficacy of other EGFR-TKIs against these tertiary EGFR mutations. Afatinib is a second-generation EGFR-TKI with broad-range sensitivity to various EGFR gene mutations [18]. Notably, afatinib’s efficacy against secondary or co-occurring mutations in vitro and in vivo, like the G724S point mutation, has been demonstrated [19]. A large retrospective study demonstrated the moderate sensitivity of osimertinib for various uncommon or compound EGFR gene mutations [20]. In this milieu, a combination of osimertinib and afatinib therapy is considered an important strategy for investigating the development of treatments for EGFR mutation-positive NSCLC. However, the molecular profiling conducted in this study did not detect uncommon EGFR mutations before treatment. The frequency of resistance mechanisms based on additional uncommon EGFR mutations might be relatively rare, requiring more participants to evaluate the efficacy of this combination against osimertinib resistance due to co-existing uncommon EGFR mutations.

Co-occurring genomic alterations contribute to the heterogeneity of driver-positive NSCLC and EGFR-targeted therapy-resistant cases [21]. Notably, TP53 mutation is a frequently co-occurring mutation in plasma samples showing a negative prognostic value in EGFR-mutation-carrying NSCLC patients. Other genomic alterations co-occurred in patients with TP53 mutations in our study and they did not respond to the EGFR-TKI combination therapy. The strategy to overcome heterogeneously mutated cancer has not been established. The subgroup analysis of the RELAY trial suggested that the addition of an anti-VEGFR-2 antibody, ramucirumab, to erlotinib might conquer the TP53-positive EGFR-mutated NSCLC [22]. The combination with antiangiogenetic agents may potentially overcome TP53 co-mutation-induced resistance, and further investigation is needed.

Furthermore, RAS-MAPK pathway activations, including KRAS or BRAF, can induce osimertinib resistance [3]. Four cases carrying mutations in the RAS-MAPK pathway genes were found in this study, and the efficacy of combination therapy was in 1 case of SD (No. 11) and 3 cases of PD (Nos. 5, 6, and 8). Further investigation is warranted to overcome these complex resistant mechanisms by combining multiple inhibitors, like KRAS allosteric inhibitors, BRAF inhibitors, and MEK inhibitors.

We detected an alternative resistant pathway due to gene amplifications, like in EGFR and ERBB2. ERBB2 amplification is an acquired resistance mechanism, with a frequency of 2 and 5% in first- and second-line settings for osimertinib, respectively [3]. Patients with ERBB2 amplification in our study did not respond to the EGFR-TKI combination therapy including the pan-HER inhibitor, afatinib. One reason may be that this patients (No. 6) had additional complex resistance mechanisms, like those conferred by BRCA-1, KRAS, NPAP-1, and TP53. Additionally, in vitro results suggested that the combination of afatinib and cetuximab may be needed to conquer the resistance due to ERBB2 amplification. Osimertinib resistance is highly diverse, and often associated with multiple gene alterations; overcoming resistance using simple combination therapy is limited. Furthermore, re-biopsy of tumors in this setting is also difficult, and the realization of precise individualized treatment using liquid biopsy is indispensable for overcoming resistance.

A key limitation of this study was the small number of participants. We could not evaluate the efficacy of the combination therapy in patients harboring T790M/trans-C797S mutations or co-existing uncommon EGFR mutations. In addition, we could not evaluate the osimertinib-resistant status in patients who achieved a PR to osimertinib plus afatinib after acquiring resistance to osimertinib monotherapy because these patients might be “non-shedders” of cfDNA. These findings indicate challenges in conquering heterogeneous EGFR-TKI resistance based on conclusions drawn from studies with a small number of patients. Furthermore, some biases could have affected the treatment outcomes in this setting. For example, in the subgroup analysis of the AURA3 trial, the mutation analysis determined via liquid biopsy showed that detectable plasma T790M status could be a prognostic factor [23]. Furthermore, high tumor burden is a predictor of cfDNA shedding and poor survival [24]. Notably, in our study population, three patients (Nos. 2, 4, and 9), in whom gene mutations or amplifications were not detected, achieved PR or SD with favorable PFS durations. Considering that undetected cfDNA may be a prognostic factor, we should evaluate this result considering selection bias. Second, the TKI-free interval has been reported as a key prognostic factor for PFS in the EGFR-TKI re-administration strategy [25]. Indeed, the osimertinib-free interval was relatively longer (77 days) in a patient showing a PR (patient number 9) than in other patients. It is difficult to avoid these biases in a setting of osimertinib resistance. A reasonable strategy for this EGFR-TKI combination is administration to treatment-naïve patients to delay resistance. The use of gefitinib and osimertinib in treatment-naïve NSCLC patients with EGFR-active mutations is being studied (NCT03122717). The results of the present study demonstrated the safety and feasibility of the combination of 20 mg afatinib and 80 mg osimertinib in a late-line setting. Further investigation of this combination in first-line therapy for patients with NSCLC and active EGFR mutations is warranted.

The combination of osimertinib and afatinib was tolerable; however, the additional antitumor effect of afatinib on osimertinib may be limited in patients with osimertinib resistance.