Background
Methods
Naked mole-rat sampling
Microbial growth conditions
MALDI-TOF-MS based microbe identification
Microbial inoculum preparation
Whole blood assay
Cytometry bead array assay
Feeding trials and analysis of biochemical constituents of the wild naked mole-rat’s diet
Data analysis
Results
Frequency of microbes isolated from wild naked mole-rats
Sites | Species | Phylum | (c.f.u./g) in range | Charactersticsa
|
---|---|---|---|---|
Colon |
Staphylococcus sciuri
| Firmicutes | 1 × 102–103
| Normal flora |
Staphylococcus cohnii spp. urealyticus
| Firmicutes | 1–2 × 103
| Normal flora | |
Staphylococcus arlettae
| Firmicutes | 3 × 102
| Normal flora | |
Staphylococcus gallinarum
| Firmicutes | 2 × 102–3 × 103
| Normal flora | |
Staphylococcus hyicus
| Firmicutes | 3 × 102
| Normal flora | |
Staphylococcus xylosus
| Firmicutes | 3 × 102
| Normal flora | |
Bacillus megaterium
| Firmicutes | 0.2–1 × 104
| Normal flora | |
Bacillus subtilus/amyloliquefaciens
| Firmicutes | 1 × 102
| Normal flora | |
Bacillus pumilus
| Firmicutes | 1 × 103
| Normal flora | |
Bacillus simplex
| Firmicutes | 2 × 103
| Normal flora | |
Paenibacillus spp. | Firmicutes | 2–3 × 102
| Normal flora | |
Paenibacillus pabuli
| Firmicutes | 1 × 102
| Normal flora | |
Bacteroides ovatus
| Bacteroidetes | 1–2 × 103
| Normal flora | |
Bacteroides thetaiotaomicron
| Bacteroidetes | 1–4 × 105
| Normal flora | |
Parabacteroides distasonis
| Bacteroidetes | 1 × 103
| Normal flora | |
Bacteroides vulgatus
| Bacteroidetes | 5 × 105
| Normal flora | |
Kluyvera ascorbata
| Proteobacteria | 3 × 103
| Normal flora | |
Klebsiella pneumoniae
| Proteobacteria | 2 × 102
| Normal flora | |
Enterobacter cloacae/asburiae
| Proteobacteria | 5 × 103–105
| Normal flora | |
Actinomyces viscosus
| Actinobacteria | 1 × 103
| Normal flora | |
Cecum |
Bacillus megaterium
| Firmicutes | 2–4 × 103
| Normal flora |
Staphylococcus gallinarum
| Firmicutes | 0.4–1 × 103
| Normal flora | |
Staphylococcus xylosus
| Firmicutes | 3 × 102
| Normal flora | |
Staphylococcus sciuri
| Firmicutes | 1 × 103
| Normal flora | |
Paenibacillus spp. | Firmicutes | 0.1–1 × 104
| Normal flora | |
Staphylococcus warneri
| Firmicutes | 1 × 103
| Normal flora | |
Brevibacillus spp. | Firmicutes | 4 × 102
| Normal flora | |
Lysinibacillus fusiformis
| Firmicutes | 2 × 102
| Normal flora | |
Streptococcus mitis/oralis
| Firmicutes | 2 × 102
| Normal flora | |
Enterococcus casseliflavus
| Firmicutes | 1 × 103
| Normal flora | |
Bacteroides ovatus
| Bacteroidetes | 0.1–1.2 × 104
| Normal flora | |
Bacteroides thetaiotaomicron
| Bacteroidetes | 0.2–1 × 105
| Normal flora | |
Bacteroides fragilis
| Bacteroidetes | 2 × 103
| Normal flora | |
Bacteroides vulgatus
| Bacteroidetes | 1 × 105
| Normal flora | |
Enterobacter cloacae/asburiae
| Proteobacteria | 0.7–2 × 103
| Normal flora | |
Actinomyces viscosus
| Actinobacteria | 1 × 102
| Normal flora | |
Rhodococcus rhodochrous
| Actinobacteria | 1 × 102
| Normal flora | |
Candida tropicalis
| Ascomycota | 1 × 103
| Normal flora | |
Feces |
Lysinibacillus fusiformis
| Firmicutes | 1 × 102
| Normal flora |
Bacillus simplex
| Firmicutes | 1 × 103
| Normal flora | |
Bacillus megaterium
| Firmicutes | 0.2–3 × 105
| Normal flora | |
Clostridium ramosum
| Firmicutes | 2 × 102
| Normal flora | |
Staphylococcus sciuri
| Firmicutes | 1–2 × 103
| Normal flora | |
Staphylococcus cohnii spp. urealyticus
| Firmicutes | 1 × 103
| Normal flora | |
Staphylococcus arlettae
| Firmicutes | 1 × 102
| Normal flora | |
Bacteriodes unifromis
| Bacteroidetes | 3 × 102
| Normal flora | |
Bacteroides thetaiotaomicron
| Bacteroidetes | 2 × 103
| Normal flora | |
Bacteriodes ovatus
| Bacteroidetes | 1 × 103
| Normal flora | |
Parabacteroides distasonis
| Bacteroidetes | 1–3 × 103
| Normal flora |
Occurrence of microbes within different intestinal compartments of wild naked mole-rats
Species | Number of positive isolates (% isolation frequency) | |||
---|---|---|---|---|
Colon (n = 10) | Cecum (n = 11) | Feces (n = 10) | Total | |
Bacillus megaterium
| 5 (50) | 5 (45.5) | 4 (40) | 14 (45.2) |
Staphylococcus sciuri
| 2 (20) | 1 (9.1) | 2 (20) | 5 (16.1) |
Bacteroides thetaiotaomicron
| 2 (20) | 3 (27.3) | 1 (10) | 6 (19.4) |
Bacillus simplex
| 1 (10) | 0 (0) | 1 (10) | 2 (6.5) |
Staphylococcus gallinarum
| 2 (20) | 2 (18.2) | 0 (0) | 4 (12.9) |
Lysinibacillus fusiformis
| 0 (0) | 1 (9.1) | 1 (10) | 2 (6.5) |
Paenibacillus spp. | 3 (30) | 2 (18.2) | 0 (0) | 5 (16.1) |
Staphylococcus cohnii spp. urealyticus
| 2 (20) | 0 (0) | 1 (10) | 3 (9.7) |
Enterobacter cloacae/asburiae
| 2 (20) | 2 (18.2) | 0 (0) | 4 (12.9) |
Bacteroides ovatus
| 1 (10) | 2 (18.2) | 2 (20) | 5 (16.1) |
Actinomyces viscosus
| 1 (10) | 1 (9.1) | 0 (0) | 2 (6.5) |
Parabacteroides distasonis
| 1 (10) | 0 (0) | 2 (20) | 3 (9.7) |
Bacteroides vulgatus
| 1 (10) | 1 (9.1) | 0 (0) | 2 (6.5) |
Staphylococcus xylosus
| 1 (10) | 1 (9.1) | 0 (0) | 2 (6.5) |
Clostridium ramosum
| 0 (0) | 0 (0) | 1 (10) | 1 (3.2) |
Bacteroides fragilis
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Staphylococcus arlettae
| 1 (10) | 0 (0) | 1 (10) | 2 (6.5) |
Brevibacillus spp. | 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Staphylococcus hyicus
| 1 (10) | 0 (0) | 0 (0) | 1 (3.2) |
Rhodococcus rhodochrous
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Bacteroides unifromis
| 0 (0) | 0 (0) | 1 (10) | 1 (3.2) |
Staphylococcus warneri
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Enterococcus casseliflavus
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Bacillus subtilus/amyloliquefaciens
| 1 (10) | 0 (0) | 0 (0) | 1 (3.2) |
Bacillus pumilus
| 1 (10) | 0 (0) | 0 (0) | 1 (3.2) |
Klebsiella pneumoniae
| 1 (10) | 0 (0) | 0 (0) | 1 (3.2) |
Kluyvera ascorbata
| 1 (10) | 0 (0) | 0 (0) | 1 (3.2) |
Streptococcus mitis/oralis
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |
Candida tropicalis
| 0 (0) | 1 (9.1) | 0 (0) | 1 (3.2) |