Background
Materials and methods
Chemicals and reagents
Human P. falciparum parasite
Processing of plant material and isolation of compounds
NMR spectroscopy analysis
Evaluation of anti-malarial activity of the compounds using SYBR green assay
Preparation of assay solutions of the compounds
Cultivation of the human P. falciparum parasite
Cultivation of trophozoites of the human P. falciparum parasite
Cultivation of schizonts of the human P. falciparum parasite
Cultivation of gametocytes of the human P. falciparum parasite
Compound plating
SYBR green assay
Cytotoxicity assay
Selectivity index(SI)
Statistical analysis
Results
Characterization of the compounds
Characterization of CA1
Characterization of CA2
Trophozoitocidal, schizonticidal and gametocytocidal activities of the compounds
Compound | Trophozoite schizont gametocyte | ||
---|---|---|---|
IC50/µM | IC50/µM | IC50/µM | |
Imperatorin | 1.57 (0.2317) | 8.97 (0.8935) | 26.92 (0.3144) |
Heptaphylline | 2.23 (0.0801) | 6.71 (1.0094) | 20.87 (0.1762) |
Artesunate | 0.00024 (0.0036) | 0.0070 (0.0013) | 0.00024 (0.00004) |
Imperatorin and heptaphylline are weakly cytotoxic against human RBCs
Compound | CC50/µM | SI of the compounds on various stages of human P. falciparum | ||
---|---|---|---|---|
Trophozoite | Schizont | Gametocyte | ||
Imperatorin | 370.37 | 235.90 | 41.28 | 13.76 |
Heptaphylline | 358.42 | 160.73 | 53.41 | 17.17 |
Artesunate | Nd | Nd | Nd | Nd |