Background
Methods
DNA methylation and gene expression data resource
Data preprocessing and differentially expressed gene screening
Gene ontology function and KEGG pathway analysis for DEGs and DMGs
Analysis of protein–protein interaction network
MiRNA–target gene regulatory network construction
Selection and mechanism analysis of candidate agents
Results
Differentially expressed genes and methylated sites screening
Gene ontology and KEGG pathway analysis
Category | Term | Count | PValue |
---|---|---|---|
Biology process | GO:0006968 ~ cellular defense response | 5 | 1.06E−03 |
GO:0006952 ~ defense response | 13 | 1.90E−03 | |
GO:0055114 ~ oxidation reduction | 12 | 7.47E−03 | |
GO:0051494 ~ negative regulation of cytoskeleton organization | 4 | 7.79E−03 | |
GO:0002696 ~ positive regulation of leukocyte activation | 5 | 7.86E−03 | |
GO:0050867 ~ positive regulation of cell activation | 5 | 9.21E−03 | |
GO:0002703 ~ regulation of leukocyte mediated immunity | 4 | 1.03E−02 | |
GO:0019835 ~ cytolysis | 3 | 1.04E−02 | |
GO:0051129 ~ negative regulation of cellular component organization | 5 | 2.10E−02 | |
GO:0006922 ~ cleavage of lamin | 2 | 2.20E−02 | |
GO:0006923 ~ cleavage of cytoskeletal proteins during apoptosis | 2 | 2.20E−02 | |
GO:0010639 ~ negative regulation of organelle organization | 4 | 2.28E−02 | |
GO:0006865 ~ amino acid transport | 4 | 2.90E−02 | |
GO:0042330 ~ taxis | 5 | 3.08E−02 | |
GO:0006935 ~ chemotaxis | 5 | 3.08E−02 | |
GO:0016310 ~ phosphorylation | 12 | 3.39E−02 | |
GO:0002694 ~ regulation of leukocyte activation | 5 | 3.46E−02 | |
GO:0051251 ~ positive regulation of lymphocyte activation | 4 | 3.51E−02 | |
GO:0001562 ~ response to protozoan | 2 | 3.64E−02 | |
GO:0043242 ~ negative regulation of protein complex disassembly | 3 | 3.68E−02 | |
GO:0002697 ~ regulation of immune effector process | 4 | 3.88E−02 | |
GO:0050865 ~ regulation of cell activation | 5 | 4.07E−02 | |
GO:0001817 ~ regulation of cytokine production | 5 | 4.52E−02 | |
GO:0006690 ~ icosanoid metabolic process | 3 | 4.72E−02 | |
KEGG pathway | hsa04650:Natural killer cell mediated cytotoxicity | 5 | 3.59E−03 |
hsa00280:Valine, leucine and isoleucine degradation | 3 | 6.42E−03 | |
hsa00140:Steroid hormone biosynthesis | 3 | 6.95E−03 | |
hsa04080:Neuroactive ligand-receptor interaction | 6 | 9.17E−03 | |
hsa00590:Arachidonic acid metabolism | 3 | 9.75E−03 | |
hsa04514:Cell adhesion molecules (CAMs) | 4 | 1.28E−02 | |
hsa04612:Antigen processing and presentation | 3 | 1.84E−02 | |
hsa00240:Pyrimidine metabolism | 3 | 2.26E−02 | |
hsa00230:Purine metabolism | 3 | 4.26E−02 | |
hsa04060:Cytokine-cytokine receptor interaction | 4 | 4.53E−02 |
Protein–protein interaction network analysis
Category | Term | Count | PValue |
---|---|---|---|
Biology process | GO:0006968 ~ cellular defense response | 5 | 9.48E−04 |
GO:0006952 ~ defense response | 13 | 1.46E−03 | |
GO:0055114 ~ oxidation reduction | 12 | 5.95E−03 | |
GO:0002696 ~ positive regulation of leukocyte activation | 5 | 7.07E−03 | |
GO:0050867 ~ positive regulation of cell activation | 5 | 8.29E−03 | |
GO:0051129 ~ negative regulation of cellular component organization | 5 | 1.90E−02 | |
GO:0016310 ~ phosphorylation | 12 | 2.78E−02 | |
GO:0042330 ~ taxis | 5 | 2.80E−02 | |
GO:0006935 ~ chemotaxis | 5 | 2.80E−02 | |
GO:0002694 ~ regulation of leukocyte activation | 5 | 3.14E−02 | |
GO:0050865 ~ regulation of cell activation | 5 | 3.71E−02 | |
GO:0001817 ~ regulation of cytokine production | 5 | 4.11E−02 | |
GO:0006796 ~ phosphate metabolic process | 13 | 4.51E−02 | |
GO:0006793 ~ phosphorus metabolic process | 13 | 4.51E−02 | |
GO:0006468 ~ protein amino acid phosphorylation | 10 | 4.96E−02 | |
GO:0009617 ~ response to bacterium | 5 | 5.00E−02 | |
KEGG pathway | hsa04650:Natural killer cell mediated cytotoxicity | 5 | 3.35E−03 |
hsa00280:Valine, leucine and isoleucine degradation | 3 | 6.19E−03 | |
hsa00140:Steroid hormone biosynthesis | 3 | 6.69E−03 | |
hsa04080:Neuroactive ligand-receptor interaction | 6 | 8.55E−03 | |
hsa00590:Arachidonic acid metabolism | 3 | 9.40E−03 | |
hsa04612:Antigen processing and presentation | 3 | 1.78E−02 | |
hsa00240:Pyrimidine metabolism | 3 | 2.19E−02 | |
hsa04514:Cell adhesion molecules (CAMs) | 3 | 3.46E−02 | |
hsa00230:Purine metabolism | 3 | 4.16E−02 | |
hsa04060:Cytokine-cytokine receptor interaction | 4 | 4.39E−02 |
Analysis of miRNA–target gene network
Term | Count | PValue | Genes |
---|---|---|---|
hsa00280:Valine, leucine and isoleucine degradation | 3 | 2.40E−03 | HADH, PCCA, ACAA1 |
hsa00240:Pyrimidine metabolism | 3 | 9.54E−03 | ENTPD5, POLR3A, ENTPD1 |
hsa04514:Cell adhesion molecules (CAMs) | 3 | 1.64E−02 | NCAM1, CDH1, SPN |
hsa04080:Neuroactive ligand-receptor interaction | 4 | 1.65E−02 | S1PR2, S1PR3, S1PR1, S1PR5 |
hsa05340:Primary immunodeficiency | 2 | 1.76E−02 | IL2RG, RFXANK |
hsa00071:Fatty acid metabolism | 2 | 1.99E−02 | HADH, ACAA1 |
hsa00230:Purine metabolism | 3 | 2.05E−02 | ENTPD5, POLR3A, ENTPD1 |
hsa04623:Cytosolic DNA-sensing pathway | 2 | 2.63E−02 | POLR3A, CCL4 |
hsa04060:Cytokine-cytokine receptor interaction | 3 | 4.27E−02 | CCL2, IL2RG, CCL4 |