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20.09.2022 | Original Article

Effects of different protocols of defocused high-power laser on the viability and migration of myoblasts—a comparative in vitro study

verfasst von: Fernanda Thomé Brochado, Belkiss Câmara Mármora, Paloma Santos Campos, Tuany Rafaeli Schmidt, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori, Lucas Gonçalves Santos, Vivian Petersen Wagner, Marcelo Lazzaron Lamers, Manoela Domingues Martins

Erschienen in: Lasers in Medical Science | Ausgabe 9/2022

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Abstract

The aim of the present study was to analyze for the first time the effect of photobiomodulation therapy (PBMT) using defocused high-power laser (DHPL) in myoblast cell line C2C12 viability and migration and compare them with low-power laser therapy. Cells were divided into 9 groups: Sham irradiation 10% fetal bovine serum (FBS); Sham irradiation 5%FBS; low-power laser 0.1 W; DHPL 810 1 W; DHPL 810 2 W; DHPL 980 1 W; DHPL 980 2 W; DHPL dual 1 W; DHPL dual 2 W. To simulate stress conditions, all groups exposed to irradiation were maintained in DMEM 5% FBS. The impact of therapies on cell viability was assessed through sulforhodamine B assay and on cells migration through scratch assays and time-lapse. Myoblast viability was not modified by PBMT protocols. All PBMT protocols were able to accelerate the scratch closure after 6 and 18 h of the first irradiation (p < 0.001). Also, an increase in migration speed, with a more pronounced effect of DHPL laser using dual-wavelength protocol with 2 W was observed (p < 0.001). In conclusion, the diverse PBMT protocols used in this study accelerated the C2C12 myoblasts migration, with 2-W dual-wavelength outstanding as the most effective protocol tested. Benefits from treating muscle injuries with PBMT appear to be related to its capacity to induce cell migration without notable impact on cell viability.

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Titel: Effects of different protocols of defocused high-power laser on the viability and migration of myoblasts—a comparative in vitro study
verfasst von: Fernanda Thomé Brochado
Belkiss Câmara Mármora
Paloma Santos Campos
Tuany Rafaeli Schmidt
Kristianne Porta Santos Fernandes
Sandra Kalil Bussadori
Lucas Gonçalves Santos
Vivian Petersen Wagner
Marcelo Lazzaron Lamers
Manoela Domingues Martins
Publikationsdatum: 20.09.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03636-7

Springer Medizin

Abstract

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