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Graefe's Archive for Clinical and Experimental Ophthalmology

Erschienen in:

20.04.2023 | Basic Science

FOXM1 promotes TGF-β2-induced injury of human lens epithelial cells by up regulating VEGFA expression

verfasst von: Xuemei Li, Wei Gao, Yanlai Zhang

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 9/2023

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Abstract

Objective

To explore whether Fork head box protein M1 (FOXM1) is involved in TGF-β2-induced injury of human lens epithelial cells and its related mechanism.

Methods

Human lens epithelium samples from cataract patients and healthy controls were collected. A cellular epithelial injury model was established by treating HLE-B3 cells with TGF-β2. QPCR, immunoblot assays were performed to detect the levels of FOXM1 in human cataract samples and the lens epithelial injury cell model. FOXM1 siRNA and pcDNA3.1-FOXM1 plasmids were transfected into the cells to knockdown and overexpress FOXM1, respectively. MTT and wound closure and transwell assays were performed to analyze cell proliferation and migration in HLE-B3 cells. Immunoblot assays were also conducted to detect the effects of FOXM1 on EMT, VEGFA and MAPK/ERK signaling.

Results

We found high expression of FOXM1 in lens tissues of cataract patients. Silencing of FOXM1 in TGF-β2-induced HLE-B3 cells suppressed cell proliferation, migration, and the EMT process. Mechanistically, we found that downregulation of FOXM1 inhibited the VEGFA/MAPK signaling pathway in TGF-β2-induced HLE-B3 cells.

Conclusion

FOXM1 promoted TGF-β2-induced injury of human lens epithelial cells (hLECs) by promoting VEGFA expression. FOXM1 could be a potential drug target for the treatment of ocular diseases.

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Titel: FOXM1 promotes TGF-β2-induced injury of human lens epithelial cells by up regulating VEGFA expression
verfasst von: Xuemei Li
Wei Gao
Yanlai Zhang
Publikationsdatum: 20.04.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 9/2023
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-023-06065-6

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FOXM1 promotes TGF-β2-induced injury of human lens epithelial cells by up regulating VEGFA expression

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