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European Archives of Oto-Rhino-Laryngology
Erschienen in: European Archives of Oto-Rhino-Laryngology 6/2012

01.06.2012

Gene expression analysis of SCC tumor cells in muscle tissue

verfasst von: Walter Hundt, Esther L. Yuh, Mykhaylo Burbelko, Andreas Kiessling, Mark D. Bednarski, Silke Steinbach

Erschienen in: European Archives of Oto-Rhino-Laryngology | Ausgabe 6/2012

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Abstract

The purpose of this study was to evaluate microarray technology of HNSCC cells in muscle tissue. 200 SCCVII tumor cells were injected intramuscularly into the right flank of ten C3H/Km mice each. One week later the animals were killed and the tissue taken out. Histology (H&E staining) and microarray of the tissue were performed. Histology showed a few tumor cells between the muscle fibers. Microarray technology showed different gene expression pattern of the muscle tissue with SCCVII cells in comparison with normal muscle tissue. Only those genes showing a fold change difference of 5 or higher were considered. Gene expression analysis revealed changes in the expression levels of SCCVII cells in muscle tissue in 220 genes. Significant gene expression differences between SCCVII cells in muscle tissue and pure muscle tissue could be seen.
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Metadaten
Titel
Gene expression analysis of SCC tumor cells in muscle tissue
verfasst von
Walter Hundt
Esther L. Yuh
Mykhaylo Burbelko
Andreas Kiessling
Mark D. Bednarski
Silke Steinbach
Publikationsdatum
01.06.2012
Verlag
Springer-Verlag
Erschienen in
European Archives of Oto-Rhino-Laryngology / Ausgabe 6/2012
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-011-1799-0

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