Background
Material and method
Study population
DNA extraction and PCR amplification
Illumina sequencing
Microbiome Analysis
Statistical analysis
Results
Basic information about fecal sample donors
Variable | Healthy control | Pancreatic cancer | Statistics | P-value | |
---|---|---|---|---|---|
Age (year) | 30.54 ± 6.75 | 67.55 ± 10.78 | t = 10.142 | 0.000 | |
BMI (kg/m2) | 20.84 ± 1.46 | 22.40 ± 2.90 | t = 1.622 | 0.113 | |
Gender | male | 3 | 17 | χ2 = 0.893 | 0.345 |
female | 6 | 12 |
Abnormal changes in gut microbes in pancreatic cancer patients
Basic information about diseased tissue sample donors and clinical data
Variable | Chronic pancreatitis | Pancreatic cancer | Statistics | P-value | |
---|---|---|---|---|---|
Age (year) | 52.35 ± 9.97 | 56 ± 7.09 | t = 1.114 | 0.275 | |
BMI(kg/m2) | 21.53 ± 2.11 | 20.56 ± 3.83 | t = 0.762 | 0.455 | |
Gender | male | 12 | 7 | χ2 = 2.602 | 0.106 |
female | 2 | 7 | |||
Tumor markers | CA125(U/L) | 12.45(117.70–5.64) | 18.82(52.39–10.32) | WMann−Whitney=56.000 | 0.538 |
CA242(IU/ml) | 3.37(4.20–1.59) | 23.23(38.70-11.65) | WMann−Whitney=1.000 | 0.002 | |
CEA(ng/ml) | 2.93(3.82–1.73) | 2.49(3.60–1.61) | WMann−Whitney=54.500 | 0.789 | |
CA19-9(U/L) | 10.90(21.55–6.80) | 274.92(466.58–72.93) | WMann−Whitney=11.000 | 0.001 |
Receiver operating characteristic(ROC) curve analysis of tumor markers
Tumor markers | AUC | P-value | 95% Confidence Interval | |
---|---|---|---|---|
Lower Bound | Upper Bound | |||
CA242 | 0.854 | 0.028 | 0.617 | 1.000 |
CA199 | 0.938 | 0.007 | 0.814 | 1.000 |
CA242 + CA199 | 0.986 | 0.014 | 0.698 | 1.000 |
Tumor markers | Sensitivity | 1-Specificity | Youden’s index | Specificity |
---|---|---|---|---|
CA242 | 0.875 | 0.167 | 0.708 | 0.833 |
CA199 | 0.75 | 0 | 0.75 | 1 |
CA242 + CA199 | 0.875 | 0 | 0.875 | 1 |