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Lasers in Medical Science

Erschienen in:

06.09.2022 | Original Article

Low-level laser therapy with different irradiation methods modulated the response of bone marrow mesenchymal stem cells in vitro

verfasst von: Daiwei Si, Bo Su, Jingwei Zhang, Kui Zhao, JinMeng Li, DeChun Chen, ShiQi Hu, Xintao Wang

Erschienen in: Lasers in Medical Science | Ausgabe 9/2022

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Abstract

Low-level laser therapy (LLLT) also known as photobiomodulation is a treatment to change cellular biological activity. The exact effects of LLLT remain unclear due to the different irradiation protocols. The purpose of this study was to investigate the effects of LLLT by three different irradiation methods on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro. BMSCs were inoculated in 24-well plates and then irradiated or not (control) with a laser using three different irradiation methods. The irradiation methods were spot irradiation, covering irradiation, and scanning irradiation according to different spot areas (0.07 cm² or 1.96 cm²) and irradiation areas (0.35 cm² or 1.96 cm²), respectively. The laser was applied three times at energy densities of 4 J/cm². The cell proliferation by CCK-8. ALP activity assay, alizarin red, and quantitative real-time polymerase chain reaction (RT-PCR) were performed to assess osteogenic differentiation and mineralization. Increases in cell proliferation was obvious following irradiation, especially for covering irradiation. The ALP activity was significantly increased in irradiated groups compared with non-irradiated control. The level of mineralization was obviously improved following irradiation, particularly for covering irradiation. RT-PCR detected significantly higher expression of ALP, OPN, OCN, and RUNX-2 in the group covering than in the others, and control is the lowest. The presented results indicate that the biostimulative effects of LLLT on BMSCs was influenced by t he irradiation method, and the covering irradiation is more favorable method to promote the proliferation and osteogenic differentiation of BMSCs.

Tuchin V (2016) Tissue optics and photonics: light-tissue interaction II. J Biomed Photonics Eng 2(3). https://doi.org/10.18287/jbpe16.02.030201

Hamblin MR (2016) Photobiomodulation or low-level laser therapy. J Biophotonics. 9(11–12):1122–4. https://doi.org/10.1002/jbio.201670113CrossRefPubMedPubMedCentral

Hosseinpour S, Fekrazad R, Arany PR, Ye Q (2019) Molecular impacts of photobiomodulation on bone regeneration: a systematic review. Prog Biophys Mol Biol. 149:147–59. https://doi.org/10.1016/j.pbiomolbio.2019.04.005CrossRefPubMed

Schneider C, Dungel P, Priglinger E, Danzer M, Schadl B, Nurnberger S (2021) The impact of photobiomodulation on the chondrogenic potential of adipose-derived stromal/stem cells. J Photochem Photobiol B. 221:112243. https://doi.org/10.1016/j.jphotobiol.2021.112243CrossRefPubMed

Oron U (2019) Photobiomodulation therapy of cells in the bone marrow: a novel therapeutic approach in cell therapy and regenerative medicine. Photobiomodul Photomed Laser Surg. 37(1):1–3. https://doi.org/10.1089/photob.2018.4543CrossRefPubMed

Safian F, Ghaffari Novin M, Nazarian H, Shams Mofarahe Z, Abdollahifar MA, Jajarmi V et al (2021) Photobiomodulation preconditioned human semen protects sperm cells against detrimental effects of cryopreservation. Cryobiology. 98:239–44. https://doi.org/10.1016/j.cryobiol.2020.09.005CrossRefPubMed

Santinoni CS, Neves APC, Almeida BFM, Kajimoto NC, Pola NM, Caliente EA et al (2021) Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization. J Biomed Mater Res A. 109(6):849–58. https://doi.org/10.1002/jbm.a.37076CrossRefPubMed

Mussttaf RA, Jenkins DFL, Jha AN (2019) Assessing the impact of low level laser therapy (LLLT) on biological systems: a review. Int J Radiat Biol. 95(2):120–43. https://doi.org/10.1080/09553002.2019.1524944CrossRefPubMed

Cardoso MV, do Vale Placa R, Sant’Ana ACP, Greghi SLA, Zangrando MSR, de Rezende MLR et al (2021) Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique. Lasers Med Sci. 36(3):541–53. https://doi.org/10.1007/s10103-020-03056-5CrossRefPubMed

10.

Pinto H, Goni Oliver P, Sanchez-Vizcaino ME (2021) The effect of photobiomodulation on human mesenchymal cells: a literature review. Aesthetic Plast Surg. 45(4):1826–42. https://doi.org/10.1007/s00266-021-02173-yCrossRefPubMed

11.

Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy - an update. Dose Response. 9(4):602–18. https://doi.org/10.2203/dose-response.11-009.HamblinCrossRefPubMedPubMedCentral

12.

AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci. 27(1):237–49. https://doi.org/10.1007/s10103-011-0885-2CrossRefPubMed

13.

Etemadi A, Sadatmansouri S, Sodeif F, Jalalishirazi F, Chiniforush N. Photobiomodulation effect of different diode wavelengths on the proliferation of human gingival fibroblast cells. Photochem Photobiol. 2021. https://doi.org/10.1111/php.1346

14.

Saghaei Bagheri H, Rasta SH, Mohammadi SM, Rahimi AAR, Movassaghpour A, Nozad Charoudeh H (2020) Low-level laser irradiation modulated viability of normal and tumor human lymphocytes in vitro. J Lasers Med Sci. 11(2):174–80. https://doi.org/10.34172/jlms.2020.29CrossRefPubMedPubMedCentral

15.

Wang L, Wu F, Liu C, Song Y, Guo J, Yang Y et al (2019) Low-level laser irradiation modulates the proliferation and the osteogenic differentiation of bone marrow mesenchymal stem cells under healthy and inflammatory condition. Lasers Med Sci. 34(1):169–78. https://doi.org/10.1007/s10103-018-2673-8CrossRefPubMed

16.

Tani A, Chellini F, Giannelli M, Nosi D, Zecchi-Orlandini S, Sassoli C (2018) Red (635 nm), near-infrared (808 nm) and Violet-Blue (405 nm) photobiomodulation potentiality on human osteoblasts and mesenchymal stromal cells: a morphological and molecular in vitro study. Int J Mol Sci. 19(7). https://doi.org/10.3390/ijms19071946

17.

Zaccara IM, Ginani F, Mota-Filho HG, Henriques AC, Barboza CA (2015) Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells. Lasers Med Sci. 30(9):2259–64. https://doi.org/10.1007/s10103-015-1803-9CrossRefPubMed

18.

Ferreira LS, Diniz IMA, Maranduba CMS, Miyagi SPH, Rodrigues M, Moura-Netto C et al (2019) Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells. Lasers Med Sci. 34(4):659–66. https://doi.org/10.1007/s10103-018-2637-zCrossRefPubMed

19.

Wu JY, Wang YH, Wang GJ, Ho ML, Wang CZ, Yeh ML et al (2012) Low-power GaAlAs laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via IGF1 and BMP2. PLoS One. 7(9):e44027. https://doi.org/10.1371/journal.pone.0044027CrossRefPubMedPubMedCentral

20.

Na S, TruongVo T, Jiang F, Joll JE, Guo Y, Utreja A et al (2018) Dose analysis of photobiomodulation therapy on osteoblast, osteoclast, and osteocyte. J Biomed Opt. 23(7):1–8. https://doi.org/10.1117/1.JBO.23.7.075008CrossRefPubMed

21.

Li C, Wang F, Zhang R, Qiao P, Liu H (2020) Comparison of proliferation and osteogenic differentiation potential of rat mandibular and femoral bone marrow mesenchymal stem cells in vitro. Stem Cells Dev. 29(11):728–36. https://doi.org/10.1089/scd.2019.0256CrossRefPubMed

22.

Amid R, Kadkhodazadeh M, Ahsaie MG, Hakakzadeh A (2014) Effect of low level laser therapy on proliferation and differentiation of the cells contributing in bone regeneration. J Lasers Med Sci. 5(4):163–70PubMedPubMedCentral

23.

Lewandowski RB, Stepinska M, Gietka A, Dobrzynska M, Lapinski MP, Trafny EA (2021) The red-light emitting diode irradiation increases proliferation of human bone marrow mesenchymal stem cells preserving their immunophenotype. Int J Radiat Biol. 97(4):553–63. https://doi.org/10.1080/09553002.2021.1876947CrossRefPubMed

24.

Khorsandi K, Hosseinzadeh R, Abrahamse H, Fekrazad R (2020) Biological responses of stem cells to photobiomodulation therapy. Curr Stem Cell Res Ther. 15(5):400–13. https://doi.org/10.2174/1574888X15666200204123722CrossRefPubMed

25.

Yin K, Zhu R, Wang S, Zhao RC (2017) Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells. Stem Cells Dev. 26(10):762–75. https://doi.org/10.1089/scd.2016.0332CrossRefPubMed

26.

Li WT, Leu YC (2007) Effects of low level red-light irradiation on the proliferation of mesenchymal stem cells derived from rat bone marrow. Annu Int Conf IEEE Eng Med Biol Soc. 2007:5830–33. https://doi.org/10.1109/IEMBS.2007.4353673CrossRefPubMed

27.

Gholami L, Parsamanesh G, Shahabi S, Jazaeri M, Baghaei K, Fekrazad R (2021) The effect of laser photobiomodulation on periodontal ligament stem cells. Photochem Photobiol. 97(4):851–9. https://doi.org/10.1111/php.13367CrossRefPubMed

28.

Li WT, Leu YC, Wu JL (2010) Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg. 28(Suppl 1):S157-65. https://doi.org/10.1089/pho.2009.2540CrossRefPubMed

29.

Chang B, Qiu H, Zhao H, Yang X, Wang Y, Ji T et al (2019) The effects of photobiomodulation on MC3T3-E1 cells via 630 nm and 810 nm light-emitting diode. Med Sci Monit. 25:8744–52. https://doi.org/10.12659/MSM.920396CrossRefPubMedPubMedCentral

30.

Miranda JM, de Arruda JAA, Moreno LMM, Gaiao WDC, do Nascimento SVB, Silva EVS et al (2020) Photobiomodulation therapy in the proliferation and differentiation of human umbilical cord mesenchymal stem cells: an in vitro study. J Lasers Med Sci. 11(4):469–74. https://doi.org/10.34172/jlms.2020.73CrossRefPubMedPubMedCentral

31.

Cavalcanti MF, Maria DA, de Isla N, Leal-Junior EC, Joensen J, Bjordal JM et al (2015) Evaluation of the proliferative effects induced by low-level laser therapy in bone marrow stem cell culture. Photomed Laser Surg. 33(12):610–6. https://doi.org/10.1089/pho.2014.3864CrossRefPubMed

32.

Horvat-Karajz K, Balogh Z, Kovacs V, Drrernat AH, Sreter L, Uher F (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med. 41(6):463–9. https://doi.org/10.1002/lsm.20791CrossRefPubMed

33.

Ruan Y, Kato H, Taguchi Y, Yamauchi N, Umeda M (2021) Irradiation by high-intensity red light-emitting diode enhances human bone marrow mesenchymal stem cells osteogenic differentiation and mineralization through Wnt/beta-catenin signaling pathway. Lasers Med Sci. 36(1):55–65. https://doi.org/10.1007/s10103-020-03002-5CrossRefPubMed

34.

Amaroli A, Agas D, Laus F, Cuteri V, Hanna R, Sabbieti MG et al (2018) The effects of photobiomodulation of 808 nm diode laser therapy at higher fluence on the in vitro osteogenic differentiation of bone marrow stromal cells. Front Physiol. 9:123. https://doi.org/10.3389/fphys.2018.00123CrossRefPubMedPubMedCentral

35.

Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA (2005) Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)–an immunohistochemical analysis. Biomaterials. 26(15):2575–84. https://doi.org/10.1016/j.biomaterials.2004.07.041CrossRefPubMed

36.

Yang D, Yi W, Wang E, Wang M (2016) Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro. Sci Rep. 6:37370. https://doi.org/10.1038/srep37370CrossRefPubMedPubMedCentral

Titel: Low-level laser therapy with different irradiation methods modulated the response of bone marrow mesenchymal stem cells in vitro
verfasst von: Daiwei Si
Bo Su
Jingwei Zhang
Kui Zhao
JinMeng Li
DeChun Chen
ShiQi Hu
Xintao Wang
Publikationsdatum: 06.09.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03624-x

Springer Medizin

Abstract

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