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25.07.2022 | Original Article

Observation and identification of autofluorescent urine crystals may be linked to a sign of urolithiasis

verfasst von: Syue-Liang Lin, Chen-Yuan Chung, Zih-Ting Chen, Chih-Chia Huang, Yun-Zhen Li, Eric Yi-Hsiu Huang, Huihua Kenny Chiang

Erschienen in: Urolithiasis | Ausgabe 5/2022

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Abstract

Urolithiasis is a common disease of the urinary system. Its recurrence rate is high and may increase medical expenses. Urine stones are composed of urine crystals and other impurities. We discovered the existence of autofluorescence in some of the urine crystals, especially in urolithiasis patients. The fluorescent molecule existed in urine crystals was verified and identified. We have applied micro-Raman and fluorescence microscopy to classify the urine crystals, used confocal laser scanning microscopy (CLSM) to examine the 3D images and spectra of autofluorescence in crystals, used Fourier-transform infrared spectroscopy (FTIR) and mass spectrometry (MS) to identify the type of fluorophore in the autofluorescent urine crystals in urine. Riboflavin was identified as one of the major fluorophores in these autofluorescent urine crystals. The prevalence rates of the autofluorescent crystals in urolithiasis patients and subjects without the history of urolithiasis were to gather statistics. We observed that 80% of urolithiasis patients had autofluorescent crystals. Contrastingly, such crystals existed in only 7% of subjects without the history of urolithiasis. The presence of autofluorescent urine crystals may be linked to a sign of urolithiasis.

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Titel: Observation and identification of autofluorescent urine crystals may be linked to a sign of urolithiasis
verfasst von: Syue-Liang Lin
Chen-Yuan Chung
Zih-Ting Chen
Chih-Chia Huang
Yun-Zhen Li
Eric Yi-Hsiu Huang
Huihua Kenny Chiang
Publikationsdatum: 25.07.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Urolithiasis / Ausgabe 5/2022
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-022-01343-0

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