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01.12.2024 | Original Article

Q-switched Nd:YAG laser protects human keratinocytes from oxidative stress and inflammation via AhR–Nrf2 pathway

verfasst von: Alessandra Fusco, Vittoria Savio, Brunella Perfetto, Maria Donniacuo, Elena Shadrina, Giovanna Donnarumma, Adone Baroni

Erschienen in: Lasers in Medical Science | Ausgabe 1/2024

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Abstract

In recent years, some treatments for esthetic and pathologic skin conditions have increasingly been based on the use of non-ablative neodymium-doped yttrium aluminum garnet (Nd:YAG) laser due to its greater penetration ability than other types of lasers, few contraindications, minimal side effects, no damage for epidermidis and the rapid recovery of the treated patients. The skin is frequently exposed to many stressors such as radiation, toxic substances, metabolites, foods, mechanical insults, and allergen exposition that cause oxidative damage and have a decisive influence on the aging process. The imbalance between reactive oxygen species, reactive nitrogen species, and the malfunctioning of the antioxidant defense system promotes the establishment of an excessive inflammatory process, which can induce various diseases including cancer and neurodegenerative disorders. The present study investigated the cytoprotective function of Q-switched Nd:YAG laser against stress aging and cell injury in HaCaT cells. We evaluated the effect of the laser on antioxidant defenses, inflammation, metalloproteinases’ expression, and the AhR-Nrf2 pathway. Q-switched Nd:YAG is able to upregulate the AhR pathway and the expression of IL-6 and TGF-β, which are involved in wound repair process, and to downregulate the expression of MMP-2 and 9, so preventing the collagen degradation. Q-switched Nd:YAG can stimulate the cellular antioxidant defenses by activating the AhR-Nrf2 system.

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Titel: Q-switched Nd:YAG laser protects human keratinocytes from oxidative stress and inflammation via AhR–Nrf2 pathway
verfasst von: Alessandra Fusco
Vittoria Savio
Brunella Perfetto
Maria Donniacuo
Elena Shadrina
Giovanna Donnarumma
Adone Baroni
Publikationsdatum: 01.12.2024
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2024
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-023-03953-5

Springer Medizin

Abstract

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