Skip to main content
Erschienen in: European Journal of Applied Physiology 5/2008

01.11.2008 | Original Article

Resistance exercise effects on blood glutathione status and plasma protein carbonyls: influence of partial vascular occlusion

verfasst von: A. H. Goldfarb, R. S. Garten, P. D. M. Chee, C. Cho, G. V. Reeves, D. B. Hollander, C. Thomas, K. S. Aboudehen, M. Francois, R. R. Kraemer

Erschienen in: European Journal of Applied Physiology | Ausgabe 5/2008

Einloggen, um Zugang zu erhalten

Abstract

Seven weight-trained males performed both light resistance with partial occlusion (LRO: 30% 1 RM) and moderate resistance (MR: 70% 1 RM) to failure to ascertain whether blood protein carbonyls (PC) and glutathione status was altered compared to partial occlusion (PO) in a counterbalanced fashion. PO was identical in duration to the LRO session and all sessions were on separate days. PC did not differ for the three conditions at PRE (0.05 nM mg protein−1). PC significantly increased for PO and MR over time and was greater than the LRO treatment at POST (0.13 nM mg protein−1). The GSSG/TGSH ratio at PRE did not differ across treatments (8%) whereas the ratio at POST was significantly elevated for PO and MR treatments (17%). In contrast, no change occurred for the LRO session at any time. These results indicate that MR to failure and PO can significantly increase blood oxidative stress but LRO did not elicit oxidative stress.
Literatur
Zurück zum Zitat Abe T, Kearns CF, Sato Y (2006) Muscle size and strength are increased following walk training with restricted venous blood flow from the leg muscle, KAATSU-walk training. J Appl Physiol 100:1460–1466PubMedCrossRef Abe T, Kearns CF, Sato Y (2006) Muscle size and strength are increased following walk training with restricted venous blood flow from the leg muscle, KAATSU-walk training. J Appl Physiol 100:1460–1466PubMedCrossRef
Zurück zum Zitat Alessio HM, Hagerman AE, Fulkerson BK et al (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 32:1576–1581PubMedCrossRef Alessio HM, Hagerman AE, Fulkerson BK et al (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 32:1576–1581PubMedCrossRef
Zurück zum Zitat Andersen ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 113:548–555CrossRef Andersen ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 113:548–555CrossRef
Zurück zum Zitat Bloomer RJ, Fry AC, Falvo MJ et al (2007) Protein carbonyls are acutely elevated following single set anaerobic exercise in resistance trained men. J Sci Med Sport 10:411–417PubMedCrossRef Bloomer RJ, Fry AC, Falvo MJ et al (2007) Protein carbonyls are acutely elevated following single set anaerobic exercise in resistance trained men. J Sci Med Sport 10:411–417PubMedCrossRef
Zurück zum Zitat Burgomaster KA, Moore DR, Schofield LM et al (2003) Resistance training with vascular occlusion: metabolic adaptations in human muscle. Med Sci Sports Exerc 35:1203–1208PubMedCrossRef Burgomaster KA, Moore DR, Schofield LM et al (2003) Resistance training with vascular occlusion: metabolic adaptations in human muscle. Med Sci Sports Exerc 35:1203–1208PubMedCrossRef
Zurück zum Zitat Doussett E, Steinberg EG, Faucher M et al (2002) Acute hypoxia does not increase the oxidative stress in resting and contracting skeletal muscle. Free Rad Res 36:701–704CrossRef Doussett E, Steinberg EG, Faucher M et al (2002) Acute hypoxia does not increase the oxidative stress in resting and contracting skeletal muscle. Free Rad Res 36:701–704CrossRef
Zurück zum Zitat Durand RJ, Castracane VD, Hollander DB et al (2003) Hormonal responses from concentric and eccentric muscle contractions. Med Sci Sports Exerc 35:937–943PubMedCrossRef Durand RJ, Castracane VD, Hollander DB et al (2003) Hormonal responses from concentric and eccentric muscle contractions. Med Sci Sports Exerc 35:937–943PubMedCrossRef
Zurück zum Zitat Goldfarb AH, Bloomer RJ, McKenzie MJ (2005) Combined antioxidant treatment effects on blood oxidative stress to eccentric exercise. Med Sci Sports Exerc 37:234–239PubMedCrossRef Goldfarb AH, Bloomer RJ, McKenzie MJ (2005) Combined antioxidant treatment effects on blood oxidative stress to eccentric exercise. Med Sci Sports Exerc 37:234–239PubMedCrossRef
Zurück zum Zitat Gute DC, Ishida T, Yarimizu K et al (1998) Inflammatory response to ischemia and reperfusion in skeletal muscle. Mol Cell Biochem 179:169–187PubMedCrossRef Gute DC, Ishida T, Yarimizu K et al (1998) Inflammatory response to ischemia and reperfusion in skeletal muscle. Mol Cell Biochem 179:169–187PubMedCrossRef
Zurück zum Zitat Hudson MB, Hosick PA, McCaulley GO et al (2008) The effect of resistance exercise on humoral markers of oxidative stress. Med Sci Sports Exerc 40:542–548PubMed Hudson MB, Hosick PA, McCaulley GO et al (2008) The effect of resistance exercise on humoral markers of oxidative stress. Med Sci Sports Exerc 40:542–548PubMed
Zurück zum Zitat Jackson AS, Pollock ML (1985) Practical assessment of body composition. J Sports Med Phys Fitness 13:76–90 Jackson AS, Pollock ML (1985) Practical assessment of body composition. J Sports Med Phys Fitness 13:76–90
Zurück zum Zitat Korthuis JK, Granger DN, Townsley MI et al (1985) The role of oxygen-derived free radicals in ischaemia-induced increases in skeletal muscle vascular permeability. Circ Res 57:599–609PubMed Korthuis JK, Granger DN, Townsley MI et al (1985) The role of oxygen-derived free radicals in ischaemia-induced increases in skeletal muscle vascular permeability. Circ Res 57:599–609PubMed
Zurück zum Zitat Kraemer WJ, Ratamess NA (2005) Hormonal responses and adaptations to resistance exercise and training. Sports Med 35:339–361PubMedCrossRef Kraemer WJ, Ratamess NA (2005) Hormonal responses and adaptations to resistance exercise and training. Sports Med 35:339–361PubMedCrossRef
Zurück zum Zitat Kraemer WJ, Marchitelli L, Gordon SE et al (1990) Hormonal and growth factor responses to heavy resistance exercise protocols. J Appl Physiol 69:1442–1450PubMed Kraemer WJ, Marchitelli L, Gordon SE et al (1990) Hormonal and growth factor responses to heavy resistance exercise protocols. J Appl Physiol 69:1442–1450PubMed
Zurück zum Zitat Lowry OH, Rosenbrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Bio Chem 193:265–275 Lowry OH, Rosenbrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Bio Chem 193:265–275
Zurück zum Zitat McBride JM, Kraemer WJ, Triplett-McBride T et al (1998) Effect of resistance exercise on free radical production. Med Sci Sports Exerc 30:67–72PubMed McBride JM, Kraemer WJ, Triplett-McBride T et al (1998) Effect of resistance exercise on free radical production. Med Sci Sports Exerc 30:67–72PubMed
Zurück zum Zitat McDonagh MJN, Davies CTM (1984) Adaptive response of mammalian skeletal muscle to exercise with high loads. Eur J Appl Physiol 52:139–155CrossRef McDonagh MJN, Davies CTM (1984) Adaptive response of mammalian skeletal muscle to exercise with high loads. Eur J Appl Physiol 52:139–155CrossRef
Zurück zum Zitat Reeves GV, Kraemer RR, Hollander DB et al (2006) Comparison of hormone responses following light resistance exercise with partial occlusion and moderately difficult resistance exercise without occlusion. J Appl Physiol 101:1616–1622PubMedCrossRef Reeves GV, Kraemer RR, Hollander DB et al (2006) Comparison of hormone responses following light resistance exercise with partial occlusion and moderately difficult resistance exercise without occlusion. J Appl Physiol 101:1616–1622PubMedCrossRef
Zurück zum Zitat Rietjens SJ, Beelen M, Koopman R et al (2007) A single session of resistance exercise induces oxidative damage in untrained men. Med Sci Sports Exerc 39:2145–2151PubMedCrossRef Rietjens SJ, Beelen M, Koopman R et al (2007) A single session of resistance exercise induces oxidative damage in untrained men. Med Sci Sports Exerc 39:2145–2151PubMedCrossRef
Zurück zum Zitat Takarada Y, Nakamura Y, Aruga S et al (2000a) Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol 88:61–65PubMed Takarada Y, Nakamura Y, Aruga S et al (2000a) Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol 88:61–65PubMed
Zurück zum Zitat Takarada Y, Takazawa H, Sato Y et al (2000b) Effects of resistance exercise combined with moderate vascular occlusion on muscular function in humans. J Appl Physiol 88:2097–2106PubMed Takarada Y, Takazawa H, Sato Y et al (2000b) Effects of resistance exercise combined with moderate vascular occlusion on muscular function in humans. J Appl Physiol 88:2097–2106PubMed
Zurück zum Zitat Tsutsumi YM, Yokoyama T, Horikawa Y et al (2007) Reactive oxygen species trigger ischemic and pharmacological postconditioning: in vivo and in vitro characterization. Life Sci 81:1223–1227PubMedCrossRef Tsutsumi YM, Yokoyama T, Horikawa Y et al (2007) Reactive oxygen species trigger ischemic and pharmacological postconditioning: in vivo and in vitro characterization. Life Sci 81:1223–1227PubMedCrossRef
Zurück zum Zitat Uchiyama S, Tsukamoto H, Yoshimura S et al (2006) Relationship between oxidative stress in muscle tissue and weight-lifting-induced muscle damage. Pflugers Arch 452:109–116PubMedCrossRef Uchiyama S, Tsukamoto H, Yoshimura S et al (2006) Relationship between oxidative stress in muscle tissue and weight-lifting-induced muscle damage. Pflugers Arch 452:109–116PubMedCrossRef
Zurück zum Zitat Winterbourn CC, Buss IH (1999) Protein carbonyl measurement by enzyme-linked immunosorbent assay. Methods Enzymol 300:106–111PubMedCrossRef Winterbourn CC, Buss IH (1999) Protein carbonyl measurement by enzyme-linked immunosorbent assay. Methods Enzymol 300:106–111PubMedCrossRef
Metadaten
Titel
Resistance exercise effects on blood glutathione status and plasma protein carbonyls: influence of partial vascular occlusion
verfasst von
A. H. Goldfarb
R. S. Garten
P. D. M. Chee
C. Cho
G. V. Reeves
D. B. Hollander
C. Thomas
K. S. Aboudehen
M. Francois
R. R. Kraemer
Publikationsdatum
01.11.2008
Verlag
Springer-Verlag
Erschienen in
European Journal of Applied Physiology / Ausgabe 5/2008
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI
https://doi.org/10.1007/s00421-008-0836-1

Weitere Artikel der Ausgabe 5/2008

European Journal of Applied Physiology 5/2008 Zur Ausgabe

Neu im Fachgebiet Arbeitsmedizin

Das Geschlechterparadoxon in der gesundheitlich beeinträchtigten Lebenszeit – Ende eines Mythos?

Beginnend mit den 1920er-Jahren hat sich eine Vorstellung über die Geschlechterdifferenzen in Gesundheit und Mortalität etabliert, die von Lorber und Moore in dem einprägsamen Satz: „Women get sicker, but men die quicker“, zusammengefasst wurde [ 1 …, S. 13]. Tatsächlich erscheinen vor dem Hintergrund der höheren Lebenserwartung der Frauen die Studienergebnisse zu den Geschlechterdifferenzen in der Morbidität überraschend, wonach Frauen im Durchschnitt einen schlechteren Gesundheitszustand aufweisen als Männer [

Gesunde Lebenserwartung: Ein kritischer Blick auf Nutzen und Potenziale des demographischen Gesundheitsindikators

Open Access Leitthema

Die demographische Alterung hat vielfältige gesellschaftliche Konsequenzen, deren Ausmaß wesentlich vom Gesundheitszustand der Bevölkerung abhängt. Um diesen analysieren und bewerten zu können, wurden spezielle Kennziffern entwickelt, die in …

Wie hat sich die Lebenserwartung ohne funktionelle Einschränkungen in Deutschland entwickelt? Eine Analyse mit Daten des Deutschen Alterssurveys (DEAS)

Deutschland erfährt, wie andere Hocheinkommensstaaten, aufgrund kontinuierlich rückläufiger Mortalität und niedriger Geburtenraten tiefgreifende demografische Veränderungen. Der demografische Wandel führt in Deutschland zu einem zunehmend höheren …

Hitzeschutz im Fokus der hessischen Betreuungs- und Pflegeaufsicht

Open Access Klimawandel Übersichtsartikel

Im Sommer 2023 kündigte das Bundesministerium für Gesundheit (BMG) einen nationalen Hitzeschutzplan an und forderte die Länder auf, zu prüfen, „ob die Warnstufen des [Deutschen Wetterdienstes] DWD mit der Durchführung von Akutmaßnahmen …