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01.12.2023 | Original Article

The effect of hepatocyte growth factor on intestinal adaption in an experimental model of short bowel syndrome

verfasst von: George Bagias, Evangelos P. Misiakos, Anestis Charalampopoulos, Nick Zavras, Stratigoula Sakellariou, Dimitrios Schizas, Igor Sukhotnik, Evangelos Giamarelos, Emmanouil Pikoulis

Erschienen in: Pediatric Surgery International | Ausgabe 1/2023

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Abstract

Purpose

Nowadays, the standard therapy for patients with short bowel syndrome is parenteral nutrition (PN). Various growth factors have been tested to achieve weaning from prolonged PN administration. We evaluated the effect of hepatocyte growth factor (HGF) on structural intestinal adaptation and cell proliferation in a rat model of SBS.

Methods

Thirty Sprague–Dawley rats were divided into three groups; group A rats (sham) underwent bowel transection, group B rats underwent a 75% bowel resection, and group C rats underwent the same procedure but were treated postoperatively with HGF. Histopathologic parameters of intestinal adaptation were determined, while microarray and rt-PCR analyses of ileal RNA were also performed.

Results

Treatment with HGF resulted in significant increase in body weight, while the jejunal and ileal villus height and crypt depth were increased in HGF rats (36%, p < 0.05 and 27%, p < 0.05 respectively). Enterocyte proliferation was also significantly increased in HGF rats (21% p < 0.05). Microarray and quantitative rt-PCR analyses showed that the genes hgfac, rac 1, cdc42, and akt 1 were more than twofold up-regulated after HGF treatment.

Conclusion

HGF emerges as a growth factor that enhances intestinal adaptation. The future use of HGF may potentially reduce the requirement for PN in SBS patients.

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Titel: The effect of hepatocyte growth factor on intestinal adaption in an experimental model of short bowel syndrome
verfasst von: George Bagias
Evangelos P. Misiakos
Anestis Charalampopoulos
Nick Zavras
Stratigoula Sakellariou
Dimitrios Schizas
Igor Sukhotnik
Evangelos Giamarelos
Emmanouil Pikoulis
Publikationsdatum: 01.12.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 1/2023
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05341-6

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The effect of hepatocyte growth factor on intestinal adaption in an experimental model of short bowel syndrome