Introduction
Material and methods
Experimental design
Participants
Demographic characteristics
Clinical laboratory values
Microvascular function
Macrovascular function
Statistical analysis
Results
Participant characteristics
Variable | Users of e-cigarettes | Non-users | p value |
---|---|---|---|
N | 21 | 21 | – |
Sex (M/F) | 10/11 | 9/12 | 0.624 |
Age (years) | 23 ± 3 | 25 ± 5 | 0.126 |
Height (cm) | 172 ± 9 | 169 ± 9 | 0.313 |
Weight (kg) | 69 ± 15 | 70 ± 20 | 0.911 |
BMI (kg/m2) | 23.2 ± 4.0 | 22.9 ± 9.1 | 0.843 |
Waist/hip ratio | 0.8 ± 0.1 | 0.7 ± 0.1 | 0.423 |
Heart rate (bpm) | 65 ± 7 | 67 ± 7 | 0.448 |
SBP (mmHg) | 116 ± 11 | 114 ± 12 | 0.521 |
DBP (mmHg) | 72 ± 5 | 69 ± 10 | 0.289 |
MAP (mmHg) | 85 ± 7 | 83 ± 12 | 0.539 |
O2 sat (%) | 98 ± 1 | 99 ± 1 | 0.151 |
E-cigarette usage (years) | 3.5 ± 1.7 | 0 ± 0 | <0.001 |
E-cigarette frequency (days/month) | 25 ± 5 | 0 ± 0 | <0.001 |
Nicotine (ng/mL) | 3.8 ± 4.7 | 0.0 ± 0.0 | 0.009 |
Cotinine (ng/mL) | 156.3 ± 192.6 | 0.0 ± 0.0 | 0.007 |
TC (mg/dL) | 136 ± 73 | 121 ± 79 | 0.554 |
HDL (mg/dL) | 54 ± 14 | 40 ± 25 | 0.065 |
LDL (mg/dL) | 92 ± 32 | 64 ± 51 | 0.107 |
TRIG (mg/dL) | 104 ± 61 | 88 ± 92 | 0.608 |
GLU (mg/dL) | 90 ± 9 | 91 ± 7 | 0.878 |
HbA1C (%) | 5.1 ± 1.1 | 5.2 ± 0.4 | 0.356 |
hs-CRP (mg/L) | 1.9 ± 3.8 | 2.1 ± 3.7 | 0.740 |
Estradiol (pg/mL) | 72 ± 27 | 62 ± 18 | 0.755 |
Microvascular function
Variable | Users of e-cigarettes | Non-users | p value |
---|---|---|---|
Local thermal hyperemia | |||
BaselineLTH (PU) | 31 ± 1 | 34 ± 2 | 0.098 |
RBFLTH (PU) | 144 ± 7 | 167 ± 5 | 0.010 |
AreaLTH (PU s−1) | 183,501 ± 12,110 | 221,238 ± 10,244 | 0.022 |
BaselineLTH (PU/mmHg) | 0.33 ± 0.02 | 0.38 ± 0.03 | 0.147 |
CVCLTH (PU/mmHg) | 1.73 ± 0.09 | 1.98 ± 0.07 | 0.046 |
TTPLTH (s) | 976 ± 60 | 1030 ± 78 | 0.726 |
CVCmax (PU/mmHg) | 2.3 ± 0.1 | 2.6 ± 0.1 | 0.023 |
Post-occlusive reactive hyperemia | |||
BaselinePORH (PU) | 34 ± 1 | 38 ± 2 | 0.117 |
RBFPORH (PU) | 62 ± 3 | 76 ± 3 | 0.003 |
AreaPORH (PU s−1) | 11,913 ± 523 | 15,459 ± 1412 | 0.023 |
BaselinePORH (PU/mmHg) | 0.41 ± 0.01 | 0.43 ± 0.02 | 0.109 |
CVCPORH (PU/mmHg) | 0.74 ± 0.03 | 0.89 ± 0.05 | 0.016 |
TTPPORH (s) | 15 ± 1 | 15 ± 1 | 0.868 |
CVCPORH (%max) | 57 ± 13 | 69 ± 4 | 0.004 |
Iontophoresis acetylcholine | |||
BaselineACH (PU) | 31 ± 2 | 37 ± 3 | 0.097 |
RBFACH (PU) | 91 ± 6 | 114 ± 9 | 0.044 |
AreaACH (PU s−1) | 3,420 ± 371 | 2,898 ± 390 | 0.241 |
BaselineACH (PU/mmHg) | 0.37 ± 0.02 | 0.39 ± 0.03 | 0.116 |
CVCACH (PU/mmHg) | 1.01 ± 0.07 | 1.24 ± 0.07 | 0.037 |
TTPACH (s) | 70 ± 8 | 63 ± 7 | 0.512 |
CVCEDD (%max) | 32 ± 3 | 41 ± 3 | 0.034 |
Iontophoresis sodium nitroprusside | |||
BaselineSNP (PU) | 32 ± 2 | 37 ± 4 | 0.104 |
RBFSNP (PU) | 82 ± 10 | 68 ± 9 | 0.328 |
AreaSNP (PU s−1) | 4,660 ± 475 | 4,363 ± 331 | 0.603 |
BaselineSNP (PU/mmHg) | 0.39 ± 0.03 | 0.41 ± 0.04 | 0.135 |
CVCSNP (PU/mmHg) | 0.93 ± 0.10 | 0.77 ± 0.10 | 0.248 |
TTPSNP (s) | 78 ± 8 | 66 ± 9 | 0.333 |
CVCEID (%max) | 47 ± 4 | 35 ± 4 | 0.036 |
Control | |||
BaselineC (PU) | 39 ± 2 | 40 ± 3 | 0.173 |
RBFC (PU) | 38 ± 5 | 39 ± 3 | 0.253 |
BaselineC (PU/mmHg) | 0.38 ± 0.02 | 0.41 ± 0.05 | 0.198 |
CVCC (PU/mmHg) | 0.45 ± 0.06 | 0.49 ± 0.07 | 0.969 |
Local thermal hyperemia
Post-occlusive reactive hyperemia
Endothelial-dependent dilation
Endothelial-independent dilation
Control site
Macrovascular function
Variable | Users e-cigarettes | Non-users | p value |
---|---|---|---|
Baseline diameter (mm) | 3.47 ± 0.10 | 3.31 ± 0.12 | 0.336 |
Peak diameter (mm) | 3.77 ± 0.12 | 3.67 ± 0.13 | 0.541 |
FMD (%) | 8.5 ± 0.8 | 10.7 ± 0.9 | 0.086 |
L-FMC (%) | −1.8 ± 0.7 | −1.9 ± 0.8 | 0.864 |
c-FMD (%) | 10.1 ± 0.8 | 12.7 ± 1.1 | 0.068 |
Shear (s−1, AUC) | 52,075 ± 4610 | 44,781 ± 4041 | 0.490 |
FMD (%)/shear (s−1, AUC) | 0.282 ± 0.04 | 0.360 ± 0.03 | 0.091 |
Time to peak (s) | 52 ± 17 | 35 ± 76 | 0.327 |
Relationship between E-cigarette usage and vascular health
Variable | ≤3 years | >3 years | p value |
---|---|---|---|
Microvascular function | |||
Local thermal hyperemia | |||
BaselineLTH (PU) | 30 ± 2 | 30 ± 2 | 0.627 |
RBFLTH (PU) | 143 ± 6 | 148 ± 5 | 0.726 |
AreaLTH (PU s−1) | 179,851 ± 12,328 | 196,824 ± 14,652 | 0.414 |
BaselineLTH (PU/mmHg) | 0.33 ± 0.02 | 0.35 ± 0.03 | 0.846 |
CVCLTH (PU/mmHg) | 1.70 ± 0.12 | 1.78 ± 0.15 | 0.683 |
TTPLTH (s) | 885 ± 98 | 1066 ± 73 | 0.156 |
Post-occlusive reactive hyperemia | |||
BaselinePORH (PU) | 34 ± 1 | 34 ± 2 | 0.608 |
RBFPORH (PU) | 68 ± 4 | 56 ± 3 | 0.044 |
AreaPORH (PU s−1) | 12,649 ± 891 | 11,071 ± 779 | 0.058 |
BaselinePORH (PU/mmHg) | 0.41 ± 0.01 | 0.41 ± 0.01 | 0.809 |
CVCPORH (PU/mmHg) | 0.80 ± 0.04 | 0.70 ± 0.03 | 0.036 |
TTPPORH (s) | 17 ± 2 | 14 ± 1 | 0.250 |
Iontophoresis with acetylcholine | |||
BaselineACH (PU) | 29 ± 2 | 34 ± 3 | 0.712 |
RBFACH (PU) | 91 ± 10 | 92 ± 8 | 0.904 |
AreaACH (PU s−1) | 3175 ± 414 | 3403 ± 336 | 0.563 |
BaselineACH (PU/mmHg) | 0.35 ± 0.02 | 0.39 ± 0.04 | 0.131 |
CVCACH (PU/mmHg) | 0.99 ± 0.11 | 1.05 ± 0.07 | 0.698 |
TTPACH (s) | 78 ± 10 | 64 ± 7 | 0.371 |
Iontophoresis with sodium nitroprusside | |||
BaselineSNP (PU) | 31 ± 2 | 34 ± 6 | 0.436 |
RBFSNP (PU) | 69 ± 10 | 96 ± 12 | 0.166 |
AreaSNP (PU s−1) | 4135 ± 494 | 5250 ± 828 | 0.253 |
BaselineSNP (PU/mmHg) | 0.37 ± 0.03 | 0.41 ± 0.04 | 0.425 |
CVCSNP (PU/mmHg) | 0.85 ± 0.11 | 1.04 ± 0.17 | 0.349 |
TTPSNP (s) | 70 ± 10 | 89 ± 7 | 0.273 |
Control | |||
BaselineC (PU) | 39 ± 2 | 39 ± 3 | 0.515 |
RBFC (PU) | 37 ± 6 | 42 ± 3 | 0.787 |
BaselineC (PU/mmHg) | 0.37 ± 0.01 | 0.38 ± 0.02 | 0.634 |
CVCC (PU/mmHg) | 0.44 ± 0.09 | 0.45 ± 0.06 | 0.884 |
Macrovascular function | |||
Baseline diameter (mm) | 3.49 ± 0.16 | 3.45 ± 0.17 | 0.812 |
Peak diameter (mm) | 3.85 ± 0.17 | 3.69 ± 0.17 | 0.509 |
FMD (%) | 10.1 ± 1.0 | 6.9 ± 0.9 | 0.002 |
L-FMC (%) | −1.5 ± 0.8 | −2.1 ± 1.1 | 0.685 |
c-FMD (%) | 11.2 ± 1.4 | 9.1 ± 0.7 | 0.090 |
Shear (s−1, AUC) | 51,903 ± 6193 | 48,862 ± 7349 | 0.755 |
FMD (%)/shear (s−1, AUC) | 0.326 ± 0.06 | 0.294 ± 0.06 | 0.366 |
Time to peak (s) | 50 ± 3 | 54 ± 7 | 0.554 |