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European Journal of Applied Physiology
Erschienen in: European Journal of Applied Physiology 3/2016

01.03.2016 | Original Article

Fatigue sensation and gene expression in trained cyclists following a 40 km time trial in the heat

verfasst von: Timothy A. VanHaitsma, Alan R. Light, Kathleen C. Light, Ronald W. Hughen, Sarah Yenchik, Andrea T. White

Erschienen in: European Journal of Applied Physiology | Ausgabe 3/2016

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Abstract

Purpose

We examined the effect of race-effort cycling exercise with and without heat stress on post-exercise perceptions of fatigue and pain, as well as mRNA expression in genes related to exercise responses.

Methods

Trained cyclists (n = 20) completed 40 km time trials during temperate (TC, 21 °C) and hot (HC, 35 °C) conditions. Blood lactates were measured 1 and 5 min post-exercise. Venous blood samples and ratings of fatigue and pain perceptions were obtained at baseline and at 0.5, 8, 24, and 48 h post-exercise. Leukocyte mRNA expression was performed for metabolite detecting, adrenergic, monoamine, and immune receptors using qPCR.

Results

Significantly lower mean power (157 ± 32.3 vs 187 ± 40 W) and lactates (6.4 ± 1.7 vs 8.8 ± 3.2 and 4.2 ± 1.5 vs 6.6 ± 2.7 mmol L−1 at 1- and 5-min post-exercise) were observed for HC versus TC, respectively (p < 0.05). Increases (p < 0.05) in physical fatigue and pain perception during TTs did not differ between TC and HC (p > 0.30). Both trials resulted in significant post-exercise decreases in metabolite detecting receptors ASIC3, P2X4, TRPV1, and TRPV4; increases in adrenergic receptors α2a, α2c, and β1; decreases in adrenergic β2, the immune receptor TLR4, and dopamine (DRD4); and increases in serotonin (HTR1D) and IL-10 (p < 0.05). Post-exercise IL-6 differed between TC and HC, with significantly greater increases observed following HC (p < 0.05).

Conclusions

Both TT performances appeared to be regulated around a specific sensory perception of fatigue and pain. Heat stress may have compensated for lower lactate during HC, thereby matching changes in metabolite detecting and other mRNAs across conditions.
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Metadaten
Titel
Fatigue sensation and gene expression in trained cyclists following a 40 km time trial in the heat
verfasst von
Timothy A. VanHaitsma
Alan R. Light
Kathleen C. Light
Ronald W. Hughen
Sarah Yenchik
Andrea T. White
Publikationsdatum
01.03.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
European Journal of Applied Physiology / Ausgabe 3/2016
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI
https://doi.org/10.1007/s00421-015-3311-9

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