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Journal of Bone and Mineral Metabolism

Erschienen in:

18.11.2022 | Original Article

The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression

verfasst von: Hao Zhang, Lin Zhou, Shaopu Hu, Wei Gu, Zhiqiang Li, Jun Sun, Xiaoen Wei, Yongjun Wang

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 6/2022

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Abstract

Introduction

Osteosarcoma is the most common malignancy in children, with high morbidity worldwide. Researches indicated that long non-coding RNAs (lncRNAs) played crucial roles in various cancers. Nevertheless, study investigating lncRNA long intergenic non-protein coding RNA 1089 (LINC01089) in osteosarcoma is extremely rare. Thus, the research of LINC01089 is of great significance.

Materials and methods

qRT-PCR and western blot were done to test the expression of RNAs and proteins in osteosarcoma cells. Functional assays were carried out to evaluate biological behaviors of hFOB1.19 and osteosarcoma cells with or without LINC01089 knockdown and overexpression. In vitro and in vivo experiments in a rescue manner were performed to reveal the influences of LINC01089 and Hippo pathway on osteosarcoma cell phenotype and tumor growth.

Results

LINC01089 was down-regulated in osteosarcoma cells and overexpressing LINC01089 was validated to restrain cell growth in vitro and tumor growth in vivo. Additionally, silencing LINC01089 could exacerbate cell malignant behaviors. Correlation of LINC01089 and Hippo pathway was proved. Overexpressing LINC01089 could activate Hippo pathway to exert antitumor effects.

Conclusion

LINC01089 could restrain the progression of osteosarcoma through activating Hippo pathway.

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Titel: The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression
verfasst von: Hao Zhang
Lin Zhou
Shaopu Hu
Wei Gu
Zhiqiang Li
Jun Sun
Xiaoen Wei
Yongjun Wang
Publikationsdatum: 18.11.2022
Verlag: Springer Nature Singapore
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 6/2022
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-022-01377-9

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Abstract

Introduction

Materials and methods

Results

Conclusion

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