The effect of hypocalcemia on motor symptoms of Parkinson’s disease

Parkinson’s disease (PD) is ranked among the most disabling neurological disorders globally and is the second most prevailing neurological disease. Parkinson’s disease is a complex neurological disorder that leads to the impairment of communication, mobility and the activity of daily life (ADL) [1]. Recently, the incidence of PD has dramatically increased with the aging population expanding [2]. Around 1% of adults over age 60 years and 2.5% of adults over age 70 years suffer from PD [3].

Parkinson’s disease's most specific pathological insignia is the deficiency of dopaminergic neurons in the substantia nigra pars compacta and the ensuing depletion of dopamine in the striatum [4]. This deficiency of dopaminergic neurons is the primary cause of motor symptoms of PD. Parkinson’s disease is defined by the presence of ubiquitin and Alpha-synuclein (α-Syn) positive cytoplasmic inclusion body [5].

Different pathophysiological changes are` involved in the progression of PD, such as mitochondrial dysfunction, oxidative stress, protein aggregation and inflammation [6]. Furthermore, many environmental and genetic risk factors, as well as specific microelements’ disruption such as calcium and vitamin D have considerable roles in the deterioration of PD [7].

Calcium is an essential mineral that represents the fifth most abundant trace element in the body [8]. Calcium plays an essential role in nerve impulses transmission, muscle contraction, and relaxation. It contributes to several neurometabolic processes, synthesis and secretion of neurotransmitters, as well as oxidation–reduction in the brain parenchyma [9]. In addition, calcium has an essential role in regulation of the cytoplasmic and nuclear calcium signals that stimulate molecular pathways of several transcription factors involved in synaptic plasticity [10].

Pchitskaya et al. [11] reported considerable evidence for a potential association between calcium and onset of neurodegenerative diseases. It is well-known that significant disturbances of serum calcium level, especially hypocalcemia, could contribute to development of different neurological diseases, such as Parkinsonism [12].

This study aimed to evaluate the relation between deficiency of serum calcium levels and the deterioration of motor symptoms of PD patients.

The demographic data of the included PD patients and control subjects are illustrated in Table 1. Between patients and controls, there were no statistically significant differences regarding age (p = 0.71), gender (p = 0.82), education years (p = 0.06) or body mass index (BMI) (p = 0.16).

The clinical data of the PD patients in Table 2 showed that there were 9 PD patients (32.1%) presented with tremors as a dominant symptom and 9 (32.1%) presented with akinetic-rigid symptoms and 10 (35.7%) presented with mixed symptoms. The distribution of patients regarding modified H–Y staging was 9 patients (32.1%) in stage 2, 15 patients (53.6%) in stage 2.5 and 4 patients (14.3%) in stage 3. The mean value of the PD patients’ scores on UPDRS II was 29.71 ± 5.82, on UPDRS III was 29.32 ± 5.89 and on the S–E ADL scale was 68.92% ± 13.42.

As shown in Table 3, the total and ionized calcium levels were significantly lower in PD patients compared with control subjects. Total calcium was distributed as (8.47 ± 0.89 versus 9.28 ± 0.8, p < 0.011) and ionized calcium was distributed as (4.19 ± 0.53 versus 4.8 ± 0.35, p < 0.001).

Table 4 illustrates that 12 PD patients (42.85%) had normal calcium levels and 16 PD patients (57.15%) presented with low calcium levels. The PD patients with hypocalcemia had significant deterioration of motor symptoms compared to PD patients with normal calcium levels as there was a significant difference between them regarding their scores on the modified H–Y scale (p = 0.001). In addition, the scores of PD patients with low calcium levels were significantly higher than the scores of PD patients with normal calcium levels on UPDRS III scale (p = 0.001) and UPDRS II (p = 0.003). The scores of PD patients with hypocalcemia on S–E ADL scale indicated significant impairment of ADL in comparison with scores of PD patients with normal calcium levels (p = 0.001). There were no significant differences between PD patients with normal and PD patients with low calcium levels regarding the dominant motor symptoms (p = 0.62).

Total and ionized calcium levels of PD patients with stage 2 of the modified H–Y scale were significantly lower than total and ionized calcium levels of PD patients with stages 2.5 and 3 (p = 0.041, p = 0.039, respectively). There were no significant differences between PD patients with stage 2.5 and 3 regarding calcium levels (Table 5).

Table 6 shows that there was a significant cutoff point of total calcium level (less than 8.1 mg/dl) and (less than3.99 mg/dl) for ionized calcium level for detection the deteriorated PD patients that presented in the stages more than grade 2 of modified H–Y scale with sensitivity 80.0% and 80.0%, respectively, and specificity 95% and 97.5% respectively (p = 0.045) for total calcium level and (p = 0.037) for ionized calcium level.

The results of the present study illustrated that there was a significant association between calcium deficiency and deterioration of motor symptoms, as there were significant negative correlations between ionized calcium levels with modified H–Y scale (p = 0.019), UPDRS II patients’ scores (p = 0.001) and UPDRS III patients scores (p = 0.001). Ionized calcium had a significant positive correlation with PD patients’ scores on S–E ADL scales (p = 0.001) (Table 7).

Neurological disorders are the leading source of disability globally, and PD is one of these fast-growing disabling diseases [20]. Variable biochemical markers have fundamental roles in the pathogenesis of PD. Calcium imbalances are proposed to have obvious effects on the progression and deterioration of PD [21].

Our results showed that serum calcium level was significantly lower in PD patients compared with control subjects. These results were in agreement with the results of Meamar et al. [7] and Liu et al. [22] who found a significant decline in serum calcium levels in PD patients. According to Abo- Raya et al. [23], inactivity, rigidity and bradykinesia of PD patients, besides walking difficulties, could reduce sun exposure time, resulting in reducing the synthesis of vitamin D and reduction of calcium absorption. In the context of this topic, Soliman and colleagues [24] found that PD patients had a significantly lower vitamin D level compared to healthy control subjects. Furthermore, Tjaden [25] reported that dopaminergic neuron deficiency in the lower brain stem and cortex could disturb the control and coordination of swallowing in PD patients. Clinical evidence revealed that dysphagia in PD patients could contribute to severe complications, including dehydration, malnutrition, and serum trace elements deficiency, such as calcium [26].

Although there are no significant differences between our PD patients and control subjects regarding age, most of our patients are around 60 years old. Increasing age is considered a great risk factor for decreasing serum calcium and vitamin D levels [27]. The decline of calcium levels in old age has been attributed to reduced 1,25 (OH)₂ D synthesis in the kidney and inadequate calcium intake in the elderly [28].

There has been controversy regarding serum calcium levels in PD patients, Sato et al. [29] and Hegde et al. [30] reported a significant increase in serum calcium levels in PD patients. It is proposed that hypercalcemia in PD patients could result from prolonged immobilization with consequently accelerated bone resorption [31].

To the best of our knowledge, few studies evaluated the relationship between serum calcium level disturbances and PD motor symptoms. In this study, we tried to highlight the relation between the calcium deficiency and the severity of motor symptoms of PD using the modified H–Y scale and UPDRS (ADL) and (Motor). We found that PD patients with low serum calcium levels had a significant deterioration of motor symptoms compared to PD patients with normal calcium levels. Our results showed a significant trend showing that when the H–Y stage deteriorated, serum calcium levels became lower. These results were in agreement with Liu et al. [22], who found that serum calcium level was low in PD patients with deteriorated motor symptoms.

Strong evidence suggests that calcium deficiency could contribute to the deterioration of the motor symptoms in PD. Lujan-Martinez et al. [32] reported in their study that it is well-known that PD is characterized by reducing dopaminergic concentration in the nigrostriatal pathway. Calcium has a potent role in neuromuscular stimulation and neurotransmission. Therefore, calcium deficiency could impair the nerve impulses transmission, which could contribute to partial or total damage of dopaminergic receptors [12]. Indeed, because substantia nigra dopaminergic neurons release dopamine in a calcium activity dependent manner from presynaptic axonal site to the striatum as well as from somatodendritic area to midbrain, so calcium can mediate many physiological functions of substantia nigra dopaminergic neurons [33].

Peterlik and Cross [34] pointed to another link between hypocalcemia and the severity of PD, reporting that low serum calcium level ensues in organ-specific modulation of calcium-sensing receptor activity and initiates an accelerated passage of extracellular Ca²⁺ into a cellular compartment. Therefore, hypocalcemia could promote Ca²⁺ overload in neurons, which contributes to mitochondrial dysfunction, reducing ATP production, generating reactive oxygen species, cytochrome C release, and finally, cell death [33].

The strong relation between hypocalcemia and vitamin D could explain motor symptoms deterioration in PD with low serum calcium levels. Vitamin D increases calcium absorption from the intestines, so that hypocalcemia could result from inadequate vitamin D production and action [35]. Reduced vitamin D level has a remarkable role in the pathogenesis and deterioration of PD [24, 36]. Vitamin D deficiency leads to decreased production of neuron growth factors in substantia nigra, increased level of inflammatory biomarkers, enhancement of oxidation stress, reduction of Neurotrophic factors (NTFs), and deregulation of tyrosine hydroxylase gene expression, consequently dopamine biosynthesis disturbances [37].

The hallmark of hypocalcemia is neuromuscular irritability that can be manifested by fatigue, anxiety, paresthesia of extremities, twitching of facial muscles, muscle sluggishness and frequent muscle cramps. With a significant decrease in serum calcium, action potentials can be formed spontaneously, producing peripheral skeletal muscle contraction [38]. All these pervious clinical symptoms of hypocalcemia could worsen the rigidity and tremors of PD patients.

This study pointed out that calcium deficiency could have a remarkable role in the progression of PD. The results of that study are consistent with the possibility that calcium deficiency could deteriorate motor symptoms and daily living activities of PD patients.