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02.10.2021 | Original Article

The effects of photodynamic therapy on Vero cells and replication of herpes simplex type 1: an in vitro analysis

verfasst von: Rosângela Maria Callou Pinheiro, Maria Tereza Villela Romanos, Antonio Canabarro, Ana Cecília Aranha, Maíra Prado, Dennis de Carvalho Ferreira, Beatriz Tholt

Erschienen in: Lasers in Dental Science | Ausgabe 4/2021

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Abstract

Purpose

The aim of this study was to perform in vitro assays using epithelial cell culture of monkey kidney (Vero cell line) and herpes simplex virus type 1 (HSV-1) to evaluate the effect of photodynamic therapy in this virus infection.

Methods

It was used a culture of epithelial cells infected (I) or not infected with HSV-1 (NI). The experimental groups were defined: control cell (G1), cell with methylene blue (G2), the control HSV-1 (G3), photodynamic therapy in the cell by the virus inoculated with laser irradiation 30 J/cm² (8 s) (G4) and irradiation 100 J/cm², 70s (G5), cell inoculated by virus with laser irradiation 30 J/cm² (8 s) (G6), and cell inoculated by virus with laser irradiation 100 J/cm² (70s) (G7). The irradiation was carried out by a GaAlAs diode laser – 660 nm, and methylene blue concentration of 0.0001%. Cell viability was investigated by inverted optical microscope (Leitz). Titer was evaluated using the 50/Ml TCID (the dose that produces cytopathic effect (CPE) in 50% of cell culture) by the method of Reed and Muench.

Results

The methylene blue and photodynamic therapy were effective in viral inhibiting. The low power laser with energy densities of 30 J/cm² and 100 J/cm² showed no effect in inhibiting HSV-1. The results of cell viability were G4 > G5 = G6˃G7 > G2.

Conclusion

Photodynamic therapy proved to be effective in inhibiting HSV-1 virus. The low power laser with wavelength of 660 nm and energy density of 30 J/cm² showed better viral inhibition with higher viable cells.

Wigg MD (2021) Viroses Dermotrópicas. In: Santos NSO, Romanos MTV, Wigg MD, Couceiro JNSS. Virologia Humana. 4ª edição, Rio de Janeiro: Guanabara Koogan, 261–311.

de Carvalho RR, de Paula EF, Ramalho KM, Antunes JL, Bezinelli LM, de Magalhães MH, Pegoretti T, de Freitas PM, de Paula EC (2010) Effect of laser phototherapy on recurring herpes labialis prevention: an in vivo study. Lasers Med Sci 25:397–402. https://doi.org/10.1007/s10103-009-0717-9CrossRefPubMed

Bello-Silva MS, de Freitas PM, Aranha AC, Lage-Marques JL, Simões A, de Paula EC (2010) Low- and high-intensity lasers in the treatment of herpes simplex virus 1 infection. Photomed Laser Surg 28:135–139. https://doi.org/10.1089/pho.2008.2458CrossRefPubMed

James C, Harfouche M, Welton NJ, Turner KM, Abu-Raddad LJ, Gottlieb SL, Looker KJ (2020) Herpes simplex virus: global infection prevalence and incidence estimates, 2016. Bull World Health Organ 98(5):315–329. https://doi.org/10.2471/BLT.19.237149CrossRefPubMedPubMedCentral

Ferreira DC, Reis HLB, Cavalcante FS, Santos KRN, Passos MRL (2011) Recurrent herpes simplex infections: laser therapy as a potential tool for long-term successful treatment. Rev Soc Bras Med Trop 44:397–399. https://doi.org/10.1590/S0037-86822011000300029CrossRefPubMed

Marotti J, Aranha AC, Eduardo Cde P, Ribeiro MS (2009) Photodynamic therapy can be effective as a treatment for herpes simplex labialis. Photomed Laser Surg 27:357–363. https://doi.org/10.1089/pho.2008.2268CrossRefPubMed

Chen F, Xu H, Liu J, Cui Y, Luo X, Zhou Y, Chen Q, Jiang L (2017) Efficacy and safety of nucleoside antiviral drugs for treatment of recurrent herpes labialis: a systematic review and meta-analysis. J Oral Pathol Med 46:561–568. https://doi.org/10.1111/jop.12534CrossRefPubMed

de Paula EC, Aranha AC, Simões A, Bello-Silva MS, Ramalho KM, Esteves-Oliveira M, de Freitas PM, Marotti J, Tunér J (2014) Laser treatment of recurrent herpes labialis: a literature review. Lasers Med Sci 29:1517–1529. https://doi.org/10.1007/s10103-013-1311-8CrossRef

Ferreira DC, Martins FO, Romanos MTV (2009) Impacto do laser de baixa intensidade na supressão de infecções pelos vírus Herpes simplex 1 e 2: estudo in vitro. Rev Soc Bras Med Trop 42:82–85. https://doi.org/10.1590/S0037-86822009000100018CrossRef

10.

La Selva A, Negreiros RM, Bezerra DT, Rosa EP, Pavesi VCS, Navarro RS, Bello-Silva MS, Ramalho KM, Aranha ACC, Braz-Silva PH, Fernandes KPS, Bussadori SK, Horliana ACRT (2020) Treatment of herpes labialis by photodynamic therapy: study protocol clinical trial (SPIRIT compliant). Medicine (Baltimore) 99(12):e19500. https://doi.org/10.1097/MD.0000000000019500CrossRefPubMedPubMedCentral

11.

Prażmo EJ, Kwaśny M, Łapiński M, Mielczarek A (2016) Photodynamic therapy as a promising method used in the treatment of oral diseases. Adv Clin Exp Med 25:799–807. https://doi.org/10.17219/acem/32488CrossRefPubMed

12.

Meneguzzo DT, Lopes LA, Pallota R, Soares-Ferreira L, Lopes-Martins RÁ, Ribeiro MS (2013) Prevention and treatment of mice paw edema by near-infrared low-level laser therapy on lymph nodes. Lasers Med Sci 28:973–980. https://doi.org/10.1007/s10103-012-1163-7CrossRefPubMed

13.

Eduardo FP, Mehnert DU, Monezi TA, Zezell DM, Schubert MM, Eduardo CP, Marques MM (2007) Cultured epithelial cells response to phototherapy with low intensity laser. Lasers Surg Med 39:365–372. https://doi.org/10.1002/lsm.20481CrossRefPubMed

14.

Sassaki SLML, GL, Romanos MTV, Barreto-Bergter E. (2012) Structural characterization and anti-HSV-1 and HSV-2 activity of glycolipids from the marine algae Osmundaria obtusiloba Isolated from southeastern brazilian coast. Mar Drugs 10:918–931. https://doi.org/10.3390/md10040918CrossRefPubMedPubMedCentral

15.

Sigal M-P, Kobi E, Hadeel I, Adva H, Bertold F, Gili J (2014) A new antiviral screening method that simultaneously detects viral replication, cell viability, and cell toxicity. J Virol Methods 208:138–143. https://doi.org/10.1016/j.jviromet.2014.08.007CrossRef

16.

Nahid Samiei R, Ebrahimi S, Fani M, Ghafari S, Makvandi M, Khodadad N, Tabasi M, Teimoori A (2020) In vitro effect of some nucleoside reverse transcriptase inhibitors against HSV-1 replication. Eur Rev Med Pharmacol Sci 24:1454–1459. https://doi.org/10.26355/eurrev_202002_20204CrossRefPubMed

17.

Markoulatos P, Georgopoulou A, Siafakas N, Plakokefalos E, Tzanakaki G, Kourea-Kremastinou J (2001) Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay. J Clin Microbiol 39:4426–4432. https://doi.org/10.1128/JCM.39.12.4426-4432.2001CrossRefPubMedPubMedCentral

18.

Reed LJ, Muench H (1938) A simple method of estimating fifty per cents endpoints. Am J Hygiene 27:493–497

19.

De Clercq E, Descamps J, Verhelst G, Walker RT, Jones AS, Torrence PF, Shugar D (1980) Comparative efficacy of antiherpes drugs against different strains of herpes simplex virus. J Infect Dis 141:563–574. https://doi.org/10.1093/infdis/141.5.563CrossRefPubMed

20.

Borenfreund E, Puerner JA (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 24:119–124. https://doi.org/10.1016/0378-4274(85)90046-3CrossRefPubMed

21.

Nishimura T, Toku H, Fukuyasu H (1977) Antiviral compounds. XII. Antiviral activity of amidinohydrazones of alkoxyphenyl-substituted carbonyl compounds against influenza virus in eggs and in mice. Kitasato Arch Exp Med 50:39–46PubMed

22.

Namvar MA, Vahedi M, Abdolsamadi HR, Mirzaei A, Mohammadi Y, Azizi JF (2019) Effect of photodynamic therapy by 810 and 940 nm diode laser on herpes simplex virus 1: an in vitro study. Photodiagnosis Photodyn Ther 25:87–91. https://doi.org/10.1016/j.pdpdt.2018.11.011CrossRefPubMed

23.

Schindl A, Neumann R (1999) Low-intensity laser therapy is an effective treatment for recurrent herpes simplex infection. Results from a randomized double-blind placebo-controlled study. J Invest Dermatol 113:221–223. https://doi.org/10.1046/j.1523-1747.1999.00684.xCrossRefPubMed

24.

Ramalho KM, Luiz AC, de Paula EC, Tunér J, Magalhães RP, Gallottini Magalhães M (2011) Use of laser phototherapy on a delayed wound healing of oral mucosa previously submitted to radiotherapy: case report. Int Wound J 8:413–418. https://doi.org/10.1111/j.1742-481X.2011.00788.xCrossRefPubMedPubMedCentral

25.

Almeida-Lopes L, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184. https://doi.org/10.1002/lsm.1107CrossRefPubMed

26.

Loevschall H, Arenholt-Bindslev D (1994) Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro. Lasers Surg Med 14:347–354. https://doi.org/10.1002/lsm.1900140407CrossRefPubMed

27.

Karu T (1989) Photobiology of low-power laser effects. Health Phys. 56:691–704. https://doi.org/10.1097/00004032-198905000-00015CrossRefPubMed

28.

Donnarumma G, De Gregorio V, Fusco A, Farina E, Baroni A, Esposito V, Contaldo M, Petruzzi M, Pannone G, Serpico R (2010) Inhibition of HSV-1 replication by laser diode-irradiation: possible mechanism of action. Int J Immunopathol Pharmacol 23:1167–1176. https://doi.org/10.1177/039463201002300420CrossRefPubMed

29.

30.

Lubart R, Friedmann H, Peled I, Grossman N (1993) Light effects on fibroblast proliferation. Laser therapy 5:55–57. https://doi.org/10.5978/islsm.93-OR-05CrossRef

31.

Hamblin MR, Zahra T, Contag CH, McManus AT, Hasan T (2003) Optical monitoring and treatment of potentially lethal wound infections in vivo. J Infect Dis 187:1717–1725. https://doi.org/10.1086/375244CrossRefPubMed

32.

Wainwright M (1998) 1998) Photodynamic antimicrobial chemotherapy (PACT. J Antimicrob Chemother 42:13–28. https://doi.org/10.1093/jac/42.1.13CrossRefPubMed

33.

Harris F, Chatfield LK, Phoenix DA (2005) Phenothiazinium based photosensitisers–photodynamic agents with a multiplicity of cellular targets and clinical applications. Curr Drug Targets 6:615–627. https://doi.org/10.2174/1389450054545962CrossRefPubMed

34.

Lotufo MA, Tempestini Horliana ACR, Santana T, de Queiroz AC, Gomes AO, Motta LJ, Ferrari RAM, Dos Santos Fernandes KP, Bussadori SK (2020) Efficacy of photodynamic therapy on the treatment of herpes labialis: a systematic review. Photodiagnosis Photodyn Ther 29:101536. https://doi.org/10.1016/j.pdpdt.2019.08.018CrossRefPubMed

35.

Tunr J (2011) Laser phototherapy in dentistry. Int CE Mag Laser Dent 1:8–17

Titel: The effects of photodynamic therapy on Vero cells and replication of herpes simplex type 1: an in vitro analysis
verfasst von: Rosângela Maria Callou Pinheiro
Maria Tereza Villela Romanos
Antonio Canabarro
Ana Cecília Aranha
Maíra Prado
Dennis de Carvalho Ferreira
Beatriz Tholt
Publikationsdatum: 02.10.2021
Verlag: Springer International Publishing
Erschienen in: Lasers in Dental Science / Ausgabe 4/2021
Elektronische ISSN: 2367-2587
DOI: https://doi.org/10.1007/s41547-021-00138-7

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The effects of photodynamic therapy on Vero cells and replication of herpes simplex type 1: an in vitro analysis

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Conclusion

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