99mTc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques | springermedizin.de

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European Journal of Nuclear Medicine and Molecular Imaging

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01.02.2006 | Original Article

^99mTc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques

verfasst von: Alessio Annovazzi, Elena Bonanno, Marcello Arca, Calogero D’Alessandria, Antonella Marcoccia, Luigi G. Spagnoli, Francesco Violi, Francesco Scopinaro, Giorgio De Toma, Alberto Signore

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 2/2006

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Abstract

Purpose

Several histopathological studies have demonstrated that vulnerable plaques are enriched in inflammatory cells. The aims of this study were: (1a) to test the ability of ^99mTc-labelled interleukin-2 (^99mTc-IL2) to bind to IL2R-positive (IL2R+) cells in carotid plaques and (1b) to correlate the plaque uptake of ^99mTc-IL2, measured in vivo, with the number of IL2R+ cells within the plaque, measured ex vivo by histology (transversal study, TS), and (2) to evaluate changes in ^99mTc-IL2 uptake in plaques, before and after treatment with a statin or a hypocholesterolaemic diet (longitudinal study, LS).

Methods

Ultrasound scan was performed for plaque characterisation and localisation. Fourteen patients (16 plaques) eligible for endoarterectomy were recruited for the TS and underwent ^99mTc-IL2 scintigraphy before surgery. Nine patients (13 plaques) were recruited for the LS; these patients received atorvastatin or a standard hypocholesterolaemic diet and ^99mTc-IL2 scintigraphy was performed before and after 3 months of treatment.

Results

The degree of ^99mTc-IL2 uptake was expressed as the plaque/background (T/B) ratio. In patients from TS, T/B ratios correlated with the percentage of IL2R+ cells at histology (r=0.707; p=0.002) and the number of IL2R+ cells at flow cytometry (r=0.711; p=0.006). No correlations were observed between ultrasound scores and either scintigraphic or histological findings. In patients from the LS, the mean ^99mTc-IL2 uptake decreased in statin-treated patients (1.75±0.50 vs 2.16±0.44; p=0.012), while it was unchanged in the patients on the hypocholesterolaemic diet (2.33±0.45 vs 2.34±0.5).

Conclusion

^99mTc-IL2 accumulates in vulnerable carotid plaques; this accumulation is correlated with the amount of IL2R+ cells and is influenced by lipid-lowering treatment with a statin.

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Titel: 99mTc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques
verfasst von: Alessio Annovazzi
Elena Bonanno
Marcello Arca
Calogero D’Alessandria
Antonella Marcoccia
Luigi G. Spagnoli
Francesco Violi
Francesco Scopinaro
Giorgio De Toma
Alberto Signore
Publikationsdatum: 01.02.2006
Verlag: Springer-Verlag
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 2/2006
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-005-1899-4