Background
Methods
Sampling and virus isolation
Virus visualisation and sequencing
Sequence and phylogenetic analysis
Results
Segment length/ ORF length [nt](start nt position) | % identity at aa level (% coverage) | Putative gene function | UniprotKB (% identityc, % coverage) | ||||
---|---|---|---|---|---|---|---|
Seg | TFRV | CTFV | EYAV | CTFV | EYAV | ||
S1 | 4356/4305 (12) | 4350/4308 (14) | 4349/4308 (13) | 56.7 (98.6) | 56.8 (98.6) | RNA-dependent RNA polymerase | Q9DSQ0 (56.8, 99.8) |
S2 | 3987/3903 (35) | 3909/3630 (46) | 3934/3828 (45) | 39.1 (82.7) | 38.3 (91.9) | Putative RNA-methyltransferase | Q9ENL4 (39.4, 84.5) |
S3 | 3513a
| 3586/3549 (12) | 3585/3549 (11) | 28.9 (98.6) | 29.1 (95.7) | Membrane-spanning protein | Q9ENL3 (28.9, 99.7) |
S4–1 | 1794 | 3157/ 3084 | 3156/3084 | 41.0 (29.6)b
| 32.1 (99.2)b
| Uncharacterised protein VP4 | Q9ENL2 (43.0, 29.7) |
S4–2 | 1398 | 3157/3084 | 3156/3084 | 46.7 (72.1)b
| 47.3 (72.1)b
| Uncharacterised protein VP4 | Q9ENL2 (46.7, 72.1) |
S5 | 3041a/2577 | 2432/2256 | 2398/2256 | 30.1 (41.8) | 29.7 (45.1) | Uncharacterised protein VP5 | Q9ENL1 (31.1, 41.8) |
S8 | 2176a
| 2029/1983 | 2028/1983 | 35.3 (25.9) | 28.8 (53.4) | Uncharacterised protein VP8 | Q9ENK8 (35.3, 26.2) |
S9 | 1857/VP9: 1008, VP9': 1794 (33) | 1884/VP9: 1014, VP9': 1809 (41) | 1884/VP9: 1014, VP9': 1809 (41) | P9: 37.2 (99.7), VP9': 37.3 (94.4) | VP9: 36.3 (99.7), VP9': 36.1 (94.4) | Structural protein VP9, Non-structural protein VP9' | VP9: O93214 (37.2, 100), VP9': O55265 (37.7, 97.7) |
S10 | 1845a
| 1880/1818 | 1879/1818 | 39.5 (99.7) | 39.0 (99.7) | Microtubule-associated protein VP10 | Q9ENK7 (39.5, 99.8) |
S11 | 971a/915 | 998/759 | 1002/927 | 26.5 (89.9) | 25.3 (71.1) | Uncharacterised protein VP11 | Q96713 (25.3, 75.4) |