A pilot and comparative study between pathological and serological levels of immunoglobulin and complement among three kinds of primary glomerulonephritis

Immunoglobulin A nephropathy (IgAN), membranous nephropathy (MN) and minimal-change disease (MCD) are three common types of glomerulonephritis in China [1, 2]. So far, pathological diagnosis based on invasive renal biopsy is the criterion and the golden standard for diagnosing the sub-types of primary or secondary glomerulonephritis, which is defined by the types of dominantly accumulated and infiltrated immunoglobulin in kidney tissue, such as IgAN is known as IgA or IgA-dominant immune complex deposition in glomerular mesangial through immunofluorescence. The formation and the deposition of immunoglobulin complexes play a key role in the pathogenesis of glomerulonephritis. An animal study on the progressive of glomerulonephropathy revealed that the formation of IgM deposition was earlier than of IgA and IgG deposition, and therefore IgA and IgG might be related with the progression of glomerular lesions [3]. Zhang et al. [4] found IgA/C3 changed with the progression of IgAN, whereas a study conducted by Yang et al. [5] showed decreased serum C3 level was not a leading factor in renal progression in IgAN. Mizerska-Wasiak M et al. [6] found that the IgA/C3 ratio was a useful marker for evaluating severity of lesions by Oxford classification in children with IgAN. All of those researches indicated immunoglobulin and complements might be used in the differential diagnosis of glomerulonephritis without renal biopsies. The evidence showed serum IgA value, serum IgA/C3 ratio and serum IgG value were related with IgAN or MN, so we supposed that blood markers might imitate histopathological changes and played a predictive role in diagnosis of the diseases. However, pathological parameters and sera parameters were always kept apart analysis in the most studies. The relationship between IF intensities of immune proteins and the corresponding serum levels remained unclear, and seldom studies combine histopathological examination results and blood tests together for a predictive purpose.

This study was considered as a pilot study for integrating histopathological indicators into serum parameters for exploring the relationship of IF intensity and serum values of immunoglobulin and complement, and for screening and investigating effective indicators for the diseases.

IF intensity grades of immunoglobulin and complement (IgG, IgM, IgA, C3, C4) were evaluated immunofluorescence of renal tissue, as a typical method of immunohistochemistry [8‐10]. [As shown in Fig. 1, the classification of negative(Neg.), positive(+) and strong positive(++) was as follows: with immunofluorescence(high magnification:X200; low magnification: X10), negative(Neg.) is defined as: no fluorescence was observed in glomerular; positive(+) is defined as: fluorescence was observed by microscope with high magnification:X200, and fluorescence is faintly visible by microscope with low magnification: X10; positive(++) is defined as: fluorescence was clearly visible by microscope with high magnification:X200, and fluorescence is visible by microscope with low magnification: X10).]

Serum IgG, IgM, IgA, C3, C4 levels were tested on BNII analyzer (Siemens). Serum BUN and Crea were tested on Cobas analyzer (ROCHE). Estimated glomerular filtration rates (eGFR) (modified to Chinese population) were calculated [11].

The morbidity of IgAN, MN and MCD has increased yearly, and IgAN is still dominant in primary glomerulonephritis [1]. Pathological results play decisive role in the diagnosis of nephropathy, by which kidney diseases are classified. Primary IgA nephropathy is characterized with IgA-dominant deposition in the glomerulus [12]; in MN, the pathological characteristics is IgG-dominant deposits [13] mostly with C3 deposits [14], and IgM or IgA has also been reported in MN [15, 16]; normally, less immonoprotein depositions are found in the patients with MCD [17]; few researches find that IgA, IgM, C3 or C1q immune complex could also exist in MCD [18‐20].

Hence, the formation and the deposition of immunoprotein complexes are closely related with pathogenesis of primary glomerular nephritis. In our study, on analysis of all subjects, we found serum IgG and C3 concentrations trended to decrease with their enhanced IF grades, while serum IgA concentration elevated with the enhanced IgA IF intensity. Our analysis showed that in MN patients, serum IgG level declined with the enhanced IgG IF intensity. Interestingly, unlike changes of IgG, IgA in pathological level or serum level showed very different changes. Serum IgA value increased with the enhanced renal tissue IgA IF intensity. This may be related to the diversity mechanism of immune complex formation in IgAN and MN. Until now, the pathogenesis of IgAN remains unclear. Some scholars found that in the patients with IgAN, besides renal IgA complexes, there also was IgA complexes accumulating in the tissue of liver [12] or skin [21]. In the physiologic state, IgA can be synthesized in many tissues. IgA is composed of two subclasses as IgA1 and IgA2. Approximately 85 % IgA in serum is IgA1. IgA1 complexes are the main deposition in IgAN [22]. Therefore, the immune dysfunction in IgAN is involved not only in kidney but also in multiple systems and tissues. In our study, serum IgA (mainly IgA1) remaining high levels, that might be caused by the abnormal activation of immune system involved in multiple tissues, was correlated with the pathological IgA IF intensity grades. Multiple systems and tissues are involved in the changes. Some other observations also had conflict on initiating pathogenesis of IgAN [23, 24]. It is still unable to affirmed that which one is the initial tissue, kidney or others organs. Our analysis also indicated that C3 had synergistic effect in IgAN, which was correlated with serum IgA level. There were some reports found that IgA/C3 index, to some extent, was valuable in the diagnosis of IgAN [4, 6]. In MN patients, both serum IgG and C3 levels are reversely correlated with their respective IF intensity grades. The pathological characteristic of MN is the formation of immune complexes (mainly IgG and C3 complexes) deposit in glomerular capillary wall in situ [4]. Our analysis indicates that the IgG deposition might not source from circulating immune complexes: the formation of high levels of IgG-complexes had limited in nephridial tissue and less in other systems. In addition, the activation of complement system is also one of the factors of renal impairment. However, our results indicated that the pathogenesis of MN, unlike that of IgAN, might be an incipient and restrictive immune disease in kidney.

eGFR is a common indicator in clinic for estimation of renal function using gender, age and serum creatinine, which was modified by Ma, Y.C [11] for Chinese population. Our result showed that, there were decreased eGFR and elevated serum creatinine with positive IgA IF level, which was corresponding to trends of serum IgA level with eGFR and serum creatinine. The result indicated that the formation of IgA complexes aggravated dysfunction of kidney. Our result also showed that IgG IF intensity grade was correlated with serum creatinine, while irrelevant with eGFR. Since eGFR is calculated with several index (gender, age and serum creatinine), the factors of gender, age also affect calculation of eGFR; moreover, significant difference may be found with sample enlarged. In separate disease analysis, interestingly, we found that in IgAN patients (characteristic as positive IgA deposition), lower eGFR appeared in IgG negative patients than in IgG positive patients. As reported in some researches [8, 10, 25], in IgA nephropathy patients, IgG deposition showed at different degrees. In Nasri H′ reports, no significant association of IgG deposition with serum creatinine [10], and only C3 deposits had a significant correlation with serum creatinine [9]. In Wada Y’s study [8], proteinuria was greater in the both IgA-IgG positive group than the IgA positive group in IgAN patients and conclude that mesangial IgG deposition is associated with more severe clinical features in IgAN patients. The conflict of results of IgG and serum creatinine(and eFGR) may caused by following reasons: i. the subjects: As illustrated, the subjects included in this study were almost at the early stage of CKD1 or CKD2, and end-stage renal disease is a contraindication for renal biopsy. Besides, age might be another cause for age of IgG Neg group was 39.7(36.9–42.4) and age of positive (+) group was 32.1(27.9–36.3) in IgAN. ii. IgG positive grade: of all subjects in this study, we found no IgG strong positive(++) cases, while in Nasri H’s [10] and Wada Y’s [8] studies, there were cases involved with IgG IF strong positive grades. But from our present data, tissue IgG IF hardly showed strong positive in IgAN patients, which might caused by population diversities or IgG might played different role in pathogenesis and development of IgAN. The study implied that the positive rate of IgA and IgG complexes was relevant with renal function, in addition C3 might play roles in pathogenesis of primary glomerular nephritis, but was irrelevant with renal dysfunction.

Serum urea nitrogen and creatinine are most common indicators for estimation of renal function. In this study on IgG IF intensity grade, we found that serum creatinine decreased with increased IgG positive IF grades; while serum creatinine evaluated with increased IgA positive IF grades; same tendency was found in serological level. Serum IgG level was found uncorrelated with eGFR, nor serum urea nitrogen or creatinine. Zhang J, et al. [4] reported that serum IgA/C3 index had predictive effects on IgAN involvement. Our result showed that IgA level (from both serological and pathological levels) was closely related with renal function, and might affect prognosis of kidney. Otherwise, increased IgG level shows none of similar relationships in MN patients.

Medical diagnosis with artificial intelligence (AI) has been developed recent years. With BIG Data analysis (trend and risk analysis based on patients records and treatments), there are preliminary models set up for evaluation the risks, processes or diagnosis of disease [26, 27]. There are some diagnostic models that have now been set up and trained for differentiation of diseases based on ‘Big Data’, such as diagnosis of skin cancer by classification of skin lesions using deep convolutional neural networks [28], Big data analytics has been used in many fields in medicine, mostly in the health care of the illness or the health [29, 30]. As the golden standard for diagnosis of primary glomerular nephritis, histopatholgical examination is a invasive method including several steps of renal biopsy, sample collection, staining, and reading. However renal biopsy has limitations which are as follow: a. patients with the contraindication; b. case with medical disputes; c. adaptability and understanding of patients and their families. Our research cross contrasts several immunoprotein IF intensities and relevant serum levels in three kinds of primary glomerular nephritis, and finally acquired helpful results for understanding the relationships between pathological presentation and serological presentation of immunoproteins in kidney diseases. Furthermore, this pilot study is offering a possible method for the analysis of combination of pathology and serology.

Our study showed that the changes of immunoprotein especially IgA and IgG, indicate the different pathogenesis of IgAN and MN, furthermore pathologically or serologically increased IgA level is closely related with renal function.

The limitation of this study is the sample numbers. Each group needs to be enlarged for more convince results. And some new renal disease markers, such as cystatin C and lipoprotein phospholipase A2, are not included.