Background
Methods
Samples and DNA preparation
Preparation of standard DNA for quantitative PCR
Real-time quantitative PCR (RT Q-PCR)
Cloning and sequencing
Results
Amplification of AIB1 gene
Cell line
a
| ER status |
AIB1 copy number ratio (tumor/normal) | Number of sequence patterns |
---|---|---|---|
MCF-10A | - | 1.1 | 3 |
MCF-10A neo | - | 1.3 | 3 |
A1N4
b
| - | 2.0 | 2 |
AK-47
| - | 1.4 | 3 |
MDA-MB435
| - | 1.3 | 4 |
LCC6
| - | 2.0 | 7 |
MDA-MB157 | - | 1.1 | 6 |
MDA-MB134V | - | 1.4 | 2 |
MDA-MB231N | - | 1.2 | 2 |
HBL100 | - | 1.2 | 1 |
ZR-75-30 | - | 1.7 | 1 |
HS 578T | - | 1.9 | 2 |
HS 578BST | - | 1.1 | 2 |
MCF7
| + | 22.2 | 1 |
MCF-7 P19
| + | 18.7 | 3 |
MCF-7 P72
| + | 22.8 | 3 |
LY-2
| + | 19.9 | 2 |
R27
| + | 19.4 | 2 |
LCC1
| + | 13.5 | 3 |
LCC2
| + | 14.6 | 2 |
LCC9
| + | 15.6 | 2 |
BT474 | + | 4.9 | 2 |
BT-483 | + | 1.9 | 4 |
BT20 | + | 1.7 | 2 |
MDA-MB468 | + | 1.7 | 2 |
ZR-75-1
| + | 1.5 | 2 |
MDA-MB361 | + | 1.3 | 2 |
T47D | + | 1.0 | 5 |
MDA-MB330 | + | 0.9 | 2 |
Somatic instability of poly Q encoding region of AIB1 gene in breast cancer cell lines
Cell line
a
| amplification | Sequence patterns | Pattern | (Q)n | Frequency |
---|---|---|---|---|---|
MCF-7 | 22.3 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 7/7 |
MCF-7 P19 | 18.7 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 7/9 |
(CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 1/9 | ||
(CAG)5CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 3 | 28 | 1/9 | ||
MCF-7 P72 | 22.8 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 5/7 |
(CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/7 | ||
(CAG)3CAA(CAG)7(CAACAG)3(CAACAGCAG)2CAA | 5 | 24 | 1/7 | ||
LCC1 | 13.5 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 7/9 |
(CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/9 | ||
(CAG)3CAA(CAG)14(CAACAG)2(CAACAGCAG)2CAA | 6 | 29 | 1/9 | ||
LCC2 | 14.6 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 4/5 |
CAG(CAACAG)3(CAACAGCAG)2CAA | 7 | 14 | 1/5 | ||
LCC9 | 15.6 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 7/8 |
(CAG)3CAA(CAG)2CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 8 | 29 | 1/8 | ||
LY-2 | 19.9 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 9/10 |
(CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 1/10 | ||
R27 | 19.4 |
(CAG)
3
CAA(CAG)
9
(CAACAG)
3
(CAACAGCAG)
2
CAA
| 1 | 26 | 8/9 |
(CAG)3CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 4 | 25 | 1/9 | ||
MDA-MB435 | 1.3 | (CAG)3CAA(CAG)2CAA(CAG)11(CAACAG)2(CAACAGCAG)2CAA | 10 | 29 | 2/8 |
(CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 3/8 | ||
(CAG)3CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 1 | 26 | 2/8 | ||
(CAG)6CAA(CAG)8(CAACAG)2(CAACAGCAG)2CAA | 11 | 26 | 1/8 | ||
LCC6 | 2.0 | (CAG)3CAA(CAG)2CAA(CAG)10(CAACAG)2(CAACAGCAG)2CAA | 12 | 28 | 1/9 |
(CAG)3CAA(CAG)2CAA(CAG)6(CAACAG)3(CAACAGCAG)2CAA | 13 | 26 | 1/9 | ||
(CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 9 | 28 | 3/9 | ||
(CAG)3CAA(CAG)2CAA(CAG)10(CAACAG)3(CAACAGCAG)2CAA | 14 | 30 | 1/9 | ||
(CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 15 | 28 | 1/9 | ||
(CAG)3CAA(CAG)2CAA(CAG)11(CAACAG)2(CAACAGCAG)2CAA | 10 | 29 | 1/9 | ||
(CAG)3CAA(CAG)2CAACAGCAA(CAG)8(CAACAG)2(CAACAGCAG)2CAA | 16 | 28 | 1/9 | ||
ZR-75-1 | 1.5 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 6/7 |
(CAG)5CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 3 | 28 | 1/7 | ||
AK-47 | 1.4 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 2/8 |
(CAG)6CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 11 | 28 | 5/8 | ||
(CAG)5CAA(CAG)8(CAACAG)3(CAACAGCAG)2CAA | 15 | 27 | 1/8 | ||
A1N4 | 2.0 | (CAG)6CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 2 | 29 | 20/41 |
(CAG)4CAA(CAG)9(CAACAG)3(CAACAGCAG)2CAA | 17 | 27 | 21/41 |