Background
Results
Co-culture with radiation killed stimulating cells in vitro significantly expanded NK cells in human PBMC
Expanded NK cells are cytolytic through both natural killing and ADCC
Expanded NK cells are activated
NK cells and NK cell culture supernatants inhibited WNV replication in Vero cells
Days Post Treatment | 1 | 2 | 3 | 4 | 5 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|
Normal control | - | - | - | - | - | - | - | - | - |
WNV control | - | - | + | + | 3+ | 4+ | 4+ | 4+ | 4+ |
NK cells 10 million | - | - | - | - | - | - | - | - | - |
NK cells 1 million | - | - | - | - | + | 4+ | 4+ | 4+ | 4+ |
NK cells 0.1 million | - | - | - | + | 2+ | 4+ | 4+ | 4+ | 4+ |
NK sup 1:2 | - | - | - | - | - | - | - | - | - |
NK sup 1:10 | - | - | - | - | + | 4+ | 4+ | 4+ | 4+ |
NK sup 1:100 | - | - | - | + | 2+ | 4+ | 4+ | 4+ | 4+ |
NK sup1:1000 | - | - | - | + | 3+ | 4+ | 4+ | 4+ | 4+ |
Anti-IFN-γ antibody neutralized NK cell supernatant inhibition of WNV replication in Vero cells
Days Post Treatments | 1 | 2 | 3 | 5 | 6 | 7 | 8 | 9 | 12 | 13 |
---|---|---|---|---|---|---|---|---|---|---|
Normal control | - | - | - | - | - | - | - | - | - | - |
WNV control | - | - | - | 2+ | 4+ | 4+ | 4+ | 4+ | 4+ | 4+ |
Control Sup 1:2 | - | - | - | 2+ | 3+ | 4+ | 4+ | 4+ | 4+ | 4+ |
NK Sup 1:2 | - | - | - | - | - | - | - | - | - | - |
NK Sup 1:2 + α IFN-αa | - | - | - | - | - | - | - | - | - | - |
NK Sup 1:2 + α IFN-βa | - | - | - | - | - | - | - | - | - | - |
NK Sup 1:2 + α IFN-γa | - | - | - | 1+ | 2+ | 3+ | 3+ | 4+ | 4+ | 4+ |