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Clinical Oral Investigations

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02.07.2018 | Original Article

Antimicrobial susceptibilities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains from periodontitis patients in Morocco

verfasst von: M. Mínguez, O. K. Ennibi, P. Perdiguero, L. Lakhdar, L. Abdellaoui, M. C. Sánchez, M. Sanz, David Herrera

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2019

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Abstract

Background and objective

Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis have been frequently isolated in periodontitis patients in Morocco. Its persistence after the subgingival debridement of the biofilm has been correlated with worse clinical outcomes. The aim of this study was to evaluate the antimicrobial susceptibilities of A. actinomycetemcomitans and P. gingivalis, to amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin. In addition, microbiological profiles of patients harbouring A. actinomycetemcomitans, P. gingivalis, or both were compared.

Material and methods

In 45 consecutive periodontitis Moroccan patients, subgingival samples were taken and processed by culture. Twenty-four A. actinomycetemcomitans and 30 P. gingivalis colonies were isolated (54 strains) and susceptibility tests, using the epsilometric method, were run for amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin. Minimum inhibitory concentrations for 50 (MIC50) and 90% (MIC90) of the organisms were calculated.

Results

The prevalence of A. actinomycetemcomitans and P. gingivalis was 79.5 and 84.4%, respectively. A. actinomycetemcomitans showed susceptibility to amoxicillin, amoxicillin plus clavulanate, while 28% of the isolated strains were resistant to azithromycin and 61.7% towards metronidazole. No P. gingivalis resistance towards amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin was found.

Conclusion

A. actinomycetemcomitans and P. gingivalis were frequently detected in Moroccan patients with periodontitis, while antimicrobial resistance was only detected for A. actinomycetemcomitans to metronidazole and azithromycin.

Clinical relevance

A. actinomycetemcomitans resistance against some antimicrobials in periodontitis patients in Morocco can influence the selection of the therapeutic approaches.

Offenbacher S (1996) Periodontal diseases: pathogenesis. Ann Periodontol 1:821–878CrossRefPubMed

Thomas RZ, Loos BG, Teeuw W, Kunnen A, van Winkelhoff AJ, Abbas F (2015) Periodontitis and systemic diseases: from science to clinical practice. Ned Tijdschr Tandheelkd 122:542–548CrossRefPubMed

Llambes F, Arias-Herrera S, Caffesse R (2015) Relationship between diabetes and periodontal infection. World J Diabetes 6:927–935CrossRefPubMedPubMedCentral

Ferreira MC, Dias-Pereira AC, Branco-de-Almeida LS, Martins CC, Paiva SM (2017) Impact of periodontal disease on quality of life: a systematic review. J Periodontal Res 52:651–665CrossRefPubMed

Mira A, Simon-Soro A, Curtis MA (2017) Role of microbial communities in the pathogenesis of periodontal diseases and caries. J Clin Periodontol 44(Suppl. 18):S23–S38CrossRefPubMed

Marsh PD (2005) Dental plaque: biological significance of a biofilm and community life-style. J Clin Periodontol 32:7–15CrossRefPubMed

Haubek D, Ennibi OK, Poulsen K, Poulsen S, Benzarti N, Kilian M (2001) Early-onset periodontitis in Morocco is associated with the highly leukotoxic clone of Actinobacillus actinomycetemcomitans. J Dent Res 80:1580–1583CrossRefPubMed

Haubek D, Ennibi OK, Vaeth M, Poulsen S, Poulsen K (2009) Stability of the JP2 clone of Aggregatibacter actinomycetemcomitans. J Dent Res 88:856–860CrossRefPubMed

Haubek D, Ennibi OK, Poulsen K, Vaeth M, Poulsen S, Kilian M (2008) Risk of aggressive periodontitis in adolescent carriers of the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans in Morocco: a prospective longitudinal cohort study. Lancet 371:237–242CrossRefPubMed

10.

Minguez M, Ennibi OK, Pousa X, Lakhdar L, Abdellaoui L, Sanchez M et al (2016) Characterization of A. actinomycetemcomitans strains in subgingival samples from periodontitis subjects in Morocco. Clin Oral Investig 20:1809–1818CrossRefPubMed

11.

Haffajee AD, Socransky SS, Dibart S, Kent RL (1996) Response to periodontal therapy in patients with high or low levels of P. gingivalis, P. intermedia, R nigrescens and B. forsythus. J Clin Periodontol 23:336–345CrossRefPubMed

12.

Holt SC, Kesavalu L, Walker S, Genco CA (1999) Virulence factors of Porphyromonas gingivalis. Periodontol 2000 20:168–238CrossRefPubMed

13.

Chahboun H, Arnau MM, Herrera D, Sanz M, Ennibi OK (2015) Bacterial profile of aggressive periodontitis in Morocco: a cross-sectional study. BMC Oral Health 15:25CrossRefPubMedPubMedCentral

14.

Van der Velden U, Abbas F, Armand S, Loos BG, Timmerman MF, Van der Weijden GA et al (2006) Java project on periodontal diseases. The natural development of periodontitis: risk factors, risk predictors and risk determinants. J Clin Periodontol 33:540–548CrossRefPubMed

15.

Hirschfeld L, Wasserman B (1978) A long-term survey of tooth loss in 600 treated periodontal patients. J Periodontol 49:225–237CrossRefPubMed

16.

Renvert S, Wikstrom M, Dahlen G, Slots J, Egelberg J (1990) Effect of root debridement on the elimination of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis from periodontal pockets. J Clin Periodontol 17:345–350CrossRefPubMed

17.

Herrera D, Sanz M, Jepsen S, Needleman I, Roldan S (2002) A systematic review on the effect of systemic antimicrobials as an adjunct to scaling and root planing in periodontitis patients. J Clin Periodontol 29(Suppl 3):136–159 discussion 60-2CrossRefPubMed

18.

Herrera D, Alonso B, Leon R, Roldan S, Sanz M (2008) Antimicrobial therapy in periodontitis: the use of systemic antimicrobials against the subgingival biofilm. J Clin Periodontol 35:45–66CrossRefPubMed

19.

Ardila CM, Granada MI, Guzman IC (2010) Antibiotic resistance of subgingival species in chronic periodontitis patients. J Periodontal Res 45:557–563PubMed

20.

Herrera D, van Winkelhoff AJ, Dellemijn-Kippuw N, Winkel EG, Sanz M (2000) Beta-lactamase producing bacteria in the subgingival microflora of adult patients with periodontitis. A comparison between Spain and The Netherlands. J Clin Periodontol 27:520–525CrossRefPubMed

21.

Van Winkelhoff AJ, Gonzales DH, Winkel EG, Dellemijn-Kippuw N, Vandenbroucke-Grauls CMJE, Sanz M (2000) Antimicrobial resistance in the subgingival microflora in patients with adult periodontitis. J Clin Periodontol 27:79–86CrossRefPubMed

22.

Van Winkelhoff AJ, Herrera D, Oteo A, Sanz M (2005) Antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in The Netherlands and Spain. J Clin Periodontol 32:893–898CrossRefPubMed

23.

Soares GMS, Figueiredo LC, Faveri M, Cortelli SC, Duarte PM, Feres M (2012) Mechanisms of action of systemic antibiotics used in periodontal treatment and mechanisms of bacterial resistance to these drugs. J Appl Oral Sci 20:295–309CrossRefPubMedPubMedCentral

24.

Armitage GC (1999) Development of a classification system for periodontal diseases and conditions. Ann Periodontol 4:1–6CrossRefPubMed

25.

Mombelli A, McNabb H, Lang NP (1991) Black-pigmenting Gram-negative bacteria in periodontal disease. II. Screening strategies for detection of P. gingivalis. J Periodontal Res 26:308–313CrossRefPubMed

26.

Syed SA, Loesche WJ (1972) Survival of human dental plaque flora in various transport media. Appl Microbiol 24:638–644

27.

Alsina M, Olle E, Frias J (2001) Improved, low-cost selective culture medium for Actinobacillus actinomycetemcomitans. J Clin Microbiol 39:509–513CrossRefPubMedPubMedCentral

28.

Nachnani S, Scuteri A, Newman MG, Avanessian AB, Lomeli SL (1992) E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. J Periodontol 63:576–583CrossRefPubMed

29.

The European Committee on Antimicrobial Susceptibility Testing (2016) Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0, 2016

30.

Benrachadi L, Bouziane A, Azziman Z, Bouziane-Ouartini F, Ennibi O (2012) Screening for periodontopathogenic bacteria in severe chronic periodontitis in a Moroccan population. Med Mal Infect 42:599–602CrossRefPubMed

31.

Kulik EM, Lenkeit K, Chenaux S, Meyer J (2008) Antimicrobial susceptibility of periodontopathogenic bacteria. J Antimicrob Chemother 61:1087–1091CrossRefPubMed

32.

Japoni A, Vasin A, Noushadi S, Kiany F, Japoni S, Alborzi A (2011) Antibacterial susceptibility patterns of Porphyromonas gingivalis isolated from chronic periodontitis patients. Med Oral Patol Oral Cir Bucal 16:1031–1035CrossRef

33.

Veloo AC, Seme K, Raangs E, Rurenga P, Singadji Z, Wekema-Mulder G et al (2012) Antibiotic susceptibility profiles of oral pathogens. Int J Antimicrob Agents 40:450–454CrossRefPubMed

34.

Dahlen G, Preus HR (2017) Low antibiotic resistance among anaerobic Gram-negative bacteria in periodontitis 5 years following metronidazole therapy. Anaerobe 43:94–98CrossRefPubMed

Titel: Antimicrobial susceptibilities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains from periodontitis patients in Morocco
verfasst von: M. Mínguez
O. K. Ennibi
P. Perdiguero
L. Lakhdar
L. Abdellaoui
M. C. Sánchez
M. Sanz
David Herrera
Publikationsdatum: 02.07.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2539-2

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Antimicrobial susceptibilities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains from periodontitis patients in Morocco