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Lasers in Medical Science

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01.01.2015 | Original Article

Are the mitochondrial respiratory complexes blocked by NO the targets for the laser and LED therapy?

verfasst von: Evgeny A. Buravlev, Tatyana V. Zhidkova, Anatoly N. Osipov, Yury A. Vladimirov

Erschienen in: Lasers in Medical Science | Ausgabe 1/2015

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Abstract

Effects of laser (442 and 532 nm) and light-emitting diode (LED) (650 nm) radiation on mitochondrial respiration and mitochondrial electron transport rate (complexes II–III and IV) in the presence of nitric oxide (NO) were investigated. It was found that nitric oxide (300 nM–10 μM) suppresses mitochondrial respiration. Laser irradiation of mitochondria (442 nm, 3 J cm⁻²) partly restored mitochondrial respiration (approximately by 70 %). Irradiation with green laser (532 nm) or red LED (650 nm) in the same dose had no reliable effect. Evaluation of mitochondrial electron transport rate in complexes II–III and IV and effects of nitric oxide demonstrated almost similar sensitivity of complex II–III and IV to NO, with approximately 50 % inhibition at NO concentration of 3 μM. Subsequent laser or LED irradiation (3 J cm⁻²) showed partial recovery of electron transport only in complex IV and only under irradiation with blue light (442 nm). Our results support the hypothesis of the crucial role of cytochrome c oxidase (complex IV) in photoreactivation of mitochondrial respiration suppressed by NO.

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Titel: Are the mitochondrial respiratory complexes blocked by NO the targets for the laser and LED therapy?
verfasst von: Evgeny A. Buravlev
Tatyana V. Zhidkova
Anatoly N. Osipov
Yury A. Vladimirov
Publikationsdatum: 01.01.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1639-8

Springer Medizin

Abstract

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