Association between vertebral fractures and coronary artery calcification in current and former smokers in the ECLIPSE cohort
verfasst von:
M. J. van Dort, J. H. M. Driessen, P. Geusens, E. A. P. M. Romme, F. W. J. M. Smeenk, B. M. Rahel, J. A. Eisman, E. F. M. Wouters, J. P. W. van den Bergh
In smokers and former smokers from the ECLIPSE cohort, there is an association between prevalent vertebral fractures (VFs) and coronary artery calcification (CAC). Chest CT scans provide the opportunity to evaluate VFs and CAC, which are potentially important comorbidities, each of which is amenable to effective interventions.
Introduction
Prevalence of VFs among smokers and patients with chronic obstructive pulmonary disease (COPD) is high, and an association between CAC and osteoporosis has been described. We investigated the associations between VFs and CAC (expressed in Agatston score) in (former) smokers.
Methods
Current and former smokers from the ECLIPSE study (designed to determine underlying COPD progression mechanisms) were studied. Baseline Agatston score (zero (0), medium (1–400), or high (> 400)), baseline bone attenuation (BA), and prevalent and incident VFs (vertebrae T1–L1) were assessed on CT.
Results
A total of 586 subjects were included (mean age 59.8 ± 8.3; 62.3% men; 70.1% with COPD; 21.0% with prevalent VFs; 196 with zero, 266 with medium, and 124 with high Agatston score). Of these, 23.4% suffered incident VFs within 3 years. In multivariate models, prevalent VFs were associated with medium (1.83 [95% CI 1.01–3.30]) and with high (OR = 3.06 [1.45–6.47]) Agatston score. After adjustment for BA, prevalent VFs were still associated with high (OR = 2.47 [1.13–5.40]), but not significantly with medium Agatston score (OR = 1.57 [0.85–2.88]). Similarly, after adjustment for BA, high (OR = 2.06 [1.02–4.13]) but not medium Agatston score (OR = 1.61 [0.88–2.94]) was associated with prevalent VFs. Agatston score at baseline was not associated with short-term VF incidence.
Conclusion
In (former) smokers, there was an association between prevalent VFs and Agatston score. Chest CT scans provide the opportunity to also evaluate for VFs and CAC, which are potentially important comorbidities, each of which is amenable to effective interventions.
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Introduction
Reported prevalence of vertebral fractures (VFs) among smokers and COPD patients is high (9–79% [1‐7]) and, in the international ECLIPSE (Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints) cohort, we previously reported that short-term incidence of VFs is high in smokers and former smokers with one or more prevalent VF [8]. A recent review described an association between cardiovascular disease (CVD) and osteoporosis [9] and suggested the potential benefits from routine bone assessment in patients with CVD. Associations between emphysema and osteoporosis or bone density have also reported [4, 10, 11]. An association between emphysema and coronary artery calcification (CAC) has been reported in some studies [12, 13] but not in others [14‐16].
Although CAC itself may be clinically asymptomatic, it is associated with coronary heart disease in patients without known CHD [17‐19], cardiovascular disease [20], cardiac events [21], and mortality [22‐24]. Also, in a population of (former) smokers (National Lung Screening Trial (USA)), CAC was associated with CHD death and all-cause mortality [25]. Calcification in the coronary arteries is related to the underlying plaque burden, and calcifications in the coronary arteries, aortic arch, and carotid arteries are correlated (moderate to strong) [26].
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Chest CT scans are regularly performed to evaluate pulmonary disease and can be used to diagnose emphysema. Additionally, CT scans give the opportunity to evaluate the bone status (bone attenuation (BA) and VFs) and CAC (expressed in Agatston score).
Therefore, the aim of this study was to evaluate the associations between variables measured on chest CT scans in current or former smokers from the ECLIPSE cohort:
a)
The association between prevalent VFs and baseline Agatston score
b)
The association between baseline Agatston score and prevalent VFs
c)
The association between baseline Agatston score and incident VFs within 1 and 3 years
Methods
Subjects
The ECLIPSE (Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints) study is a non-interventional multicenter study that was started to search underlying mechanisms of disease progression in subjects with COPD and to identify biomarkers that may serve as surrogate endpoints and measures of disease progression (Clinicaltrials.gov identifier NCT00292552; GlaxoSmithKline study SCO104960).
Detailed inclusion and exclusion criteria were described elsewhere [27‐29]. In brief, current or former smokers (40–75 years old) with moderate to very severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages II–IV) or without COPD, with a smoking history of at least 10 pack years, were included. Subjects with respiratory diseases other than COPD, patients with α1-antitrypsin deficiency, with known history of significant inflammatory disease other than COPD, with an exacerbation requiring treatment within 4 weeks prior to enrolment, or subjects who used oral glucocorticosteroids (GC) at baseline were excluded.
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Measurements
Demographic and pulmonary information were collected as well as number of pack years and smoking status. Detailed information can be found elsewhere [27‐29].
CT scans were performed at full inspiration (120 kV peak, 40 mAs, 1.00 or 1.25-mm volumetric acquisition, General Electric or Siemens), at baseline, 1-year, and 3-year follow-up. CT scanners were calibrated regularly using industry and institutional standards. Emphysema, VFs, BA, and Agatston score were measured on these CT scans.
Emphysema was measured as % low attenuation area (%LAA), as described by Coxson et al. [30]. The presence of emphysema was defined by a cutoff value of > 10%LAA [30].
Sagittal reformats containing the spine were superposed to create simulated lateral X-ray images. VFs from T1 to L1 were semi-quantitatively and subsequently quantitatively (using SpineAnalyzer Morphometry software; Optasia Medical, Cheadle, UK [31‐33]) assessed on the 3-year follow-up images. VFs were classified according to the method by Genant et al. as grade 1 (mild), grade 2 (moderate), or grade 3 (severe) [34]. If VFs were diagnosed on the 3-year scan, the 1-year scan was morphometrically assessed. If VFs were diagnosed on the 1-year scan, the baseline scan was also evaluated. Incident VFs were defined as new (no VF to any grade of VF) or worsening VFs (increase in grade of VF) as previously described [8].
BA was measured semi-automatically in cubic areas of approximately 275 mm3 (slightly varying due to different voxel sizes between scanners) centered in vertebrae T4–T12. Mean BA of vertebrae T4–T12 was expressed in Hounsfield units (HU). Vertebrae that were fractured (grade 1 or higher [34]) or that showed other abnormalities were excluded from BA measurements.
Agatston score measurements are described in detail elsewhere [15, 35]. Areas of ≥ 1 mm3 with a threshold of ≥ 130 HU in the course of a coronary artery were considered CAC. Agatston score was calculated as the sum of weighted areas (depending on peak attenuation within the area) on axial slices. Three groups were made based on Agatston score at baseline: zero (0 Agatston units (AU)), medium (1–400 AU), or high Agatston score (> 400 AU).
Incident VFs within 1 and 3 years (main factor: prevalent Agatston score)
Possible confounders that were considered included age; sex; BMI; smoking status (current/former); number of pack years; presence of COPD; emphysema (> 10%LAA); and in case of incident VFs, prevalent VFs at baseline. Since associations between BA and Agatston score [36] and between BA and fractures [1, 37] have been reported, BA was included as a potential confounder.
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Statistics
a)
Association between prevalent VFs and baseline Agatston score
A chi-square test was used to compare the proportion of patients with a prevalent VF between the Agatston score groups. Logistic regression models (SAS 9.3, SAS Institute, Cary, NC, USA; LOGISTIC procedure) were used to evaluate the association between prevalent VFs (no VF, or ≥ 1 VF) and Agatston score group (zero 0 AU, medium 1–400 AU, or high > 400 AU). To compare medium vs zero Agatston score group, only subjects with medium or zero Agatston score were included in the analyses. To compare high vs zero Agatston score, only subjects with high or zero Agatston score were included.
b)
Association between baseline Agatston score and prevalent VFs
To evaluate the association between Agatston score and prevalent VFs, logistic regression models including all subjects were used.
c)
Association between Agatston score and incident VFs within 1 and 3 years
A chi-square test was used to compare the proportion of patients with an incident VF between the Agatston score groups. To evaluate the association between Agatston score and incident VFs, Cox proportional hazard models were used (PHREG procedure). When incident VFs were expressed in numbers of incident VFs (0, 1, or ≥ 2), a nominal regression model was used (LOGISTIC procedure).
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Confounders
Age, sex, and presence of COPD were included in all multivariate models; other confounders were included in the multivariate model if they independently changed the beta-coefficient of the main exposure ((a) prevalent VFs; (b) Agatston score group; (c) incident VFs) by 5% or more, or previously reported associations indicated that they should be included.
Odds ratios (ORs) and hazard ratios (HRs) are given with 95% CI. Subjects with complete availability of VF assessment and Agatston score evaluation were selected. Only 1 subject (0.2%) had missing data and was excluded from further analyses.
Results
Out of a total of 2298 ECLIPSE subjects (327 subjects without and 1971 with COPD), there were 586 subjects (411 with and 175 without COPD) with Agatston score and VF assessment available (Table 1) [8]. Of the patients with a zero Agatston score, 20 (10.2%) had one or more prevalent VFs, compared to 61 (22.9%) in the medium Agatston score group and 42 (33.9%) in the high Agatston score group (Fig. 1). The proportion of patients with a prevalent VF was significantly different across the Agatston score groups (p value < 0.001). Of the patients with a zero Agatston score, 40 (20.4%) had one or more incident VFs within 3 years, compared to 60 (22.6%) in the medium Agatston score group and 37 (29.8%) in the high Agatston score group. The proportion of patients with an incident VF over 3 years increased (but not significantly) across the Agatston score groups (p value 0.14).
Table 1
Baseline characteristics
All subjects
Zero Agatston group
Medium Agatston group
High Agatston group
n = 586
n = 196
n = 266
n = 124
Age (years: mean, sd)
59.8
8.3
55.0
7.6
60.8
7.7
65.3
6.1
Sex (M: n, %)
365
62.3
93
47.4
176
66.2
96
77.4
BMI (kg/m2: mean, sd)
26.1
4.3
26.0
4.6
26.2
4.0
26.0
4.2
With COPD (n, %)
411
70.1
106
54.1
200
75.2
105
84.7
Current smoker (n, %)
275
46.9
111
56.6
115
43.2
49
39.5
Pack years (mean, sd)
41.1
24.0
32.8
18.8
44.1
24.8
47.6
26.2
Emphysema > 10%LAA (n, %)
263
44.9
66
33.7
130
48.9
67
54.0
Bone attenuation (HU: mean, sd)
158.4
46.5
174.9
43.4
154.0
42.6
141.5
51.2
≥ 1 prevalent VF (n, %)
123
21.0
20
10.2
61
22.9
42
33.9
Incident VF within 1 year (n, %)
57
9.7
14
7.1
22
8.3
21
16.9
Incident VF within 3 years (n, %)
137
23.4
40
20.4
60
22.6
37
29.8
Baseline characteristics for all subjects and by Agatston score group. Zero 0 Agatston units (AU); medium 1–400 AU; high > 400 AU
BMI body mass index, COPD chronic obstructive pulmonary disease, %LAA % low attenuation area, HU Hounsfield units, VF vertebral fracture
Fig. 1
Proportion of patients with a prevalent VF (left panel) or 1- or 3-year incident VF (right panel) stratified by Agatston score. AU Agatston units, VF vertebral fracture
×
Association between prevalent VFs and baseline Agatston score
Of the subjects with one or more prevalent VF, 20 (16%) had zero Agatston score, 61 (50%) had medium Agatston score, and 42 (34%) had high Agatston score. In subjects without prevalent VFs, these numbers were 176 (38%), 205 (44%), and 82 (18%) respectively (Supplemental Figure 1).
In univariate models, subjects with prevalent VFs had higher odds of a medium (OR 2.62 [1.52–4.51]) or high Agatston score (OR 4.51 [2.49–8.16]) compared to subjects without VFs (Table 2). In multivariate models, subjects with prevalent VFs had higher odds of having a medium (1.83 [95% CI 1.009–3.304]) or high (OR = 3.06 [1.450–6.473]) Agatston score compared to subjects without VFs. After additional adjustment for BA, subjects with prevalent VFs still had higher odds of high (OR = 2.37 [1.06–5.31]), but not of medium Agatston score (OR = 1.56 [0.84–2.90]).
Table 2
Association between prevalent VFs and Agatston score
Univariate
Multivariate, without BA
Multivariate, with BA
OR
95% CI
OR
95% CI
OR
95% CI
Medium Agatston score
Age (per + 8 years)
2.16
[1.750–2.654]
1.84
[1.446–2.332]
1.67
[1.296–2.150]
Sex (M vs F)
2.17
[1.484–3.162]
1.79
[1.183–2.703]
1.85
[1.220–2.808]
BMI (per + 4 kg/m2)
1.05
[0.879–1.243]
–
–
Current smoker (vs former)
0.58
[0.402–0.846]
–
–
Pack years (per + 24 pack years)
1.95
[1.502–2.537]
1.37
[1.041–1.791]
1.39
[1.060–1.828]
COPD (vs no COPD)
2.57
[1.733–3.821]
1.26
[0.781–2.030]
1.27
[0.788–2.062]
BA (per − 46 HU)
1.68
[1.367–2.072]
–
1.30
[1.024–1.664]
≥ 1 prevalent VF (vs none)
2.62
[1.520–4.510]
1.83
[1.009–3.304]
1.57
[0.853–2.883]
Emphysema (vs “no emphysema”)
1.88
[1.286–2.757]
–
–
High Agatston score
Age (per + 8 years)
4.81
[3.373–6.854]
3.97
[2.718–5.809]
3.69
[2.512–5.412]
Sex (M vs F)
3.80
[2.290–6.297]
2.75
[1.473–5.118]
3.08
[1.616–5.885]
BMI (per + 4 kg/m2)
1.01
[0.828–1.241]
–
–
Current smoker (vs former)
0.50
[0.317–0.791]
–
–
Pack years (per + 24 pack years)
2.17
[1.610–2.914]
1.60
[1.124–2.272]
1.57
[1.099–2.237]
COPD (vs no COPD)
4.69
[2.670–8.244]
1.39
[0.665–2.916]
1.38
[0.657–2.886]
BA (per − 46 HU)
2.12
[1.630–2.751]
1.43
[1.031–1.971]
≥ 1 prevalent VF (vs none)
4.51
[2.490–8.158]
3.06
[1.450–6.473]
2.47
[1.128–5.403]
Emphysema (vs “no emphysema”)
2.32
[1.460–3.671]
–
–
Significant ORs are in italic format. For continuous variables, ORs are given per standard deviation of the total population. Multivariate: confounders included according to > 5% rule (age, sex, and presence of COPD were included in all models). Reference group is the zero Agatston score group
OR odds ratio, 95% CI 95% confidence interval, BMI body mass index, COPD chronic obstructive pulmonary disease, BA bone attenuation, HU Hounsfield units, VF vertebral fracture, %LAA % low attenuation area
In univariate models, odds of medium or high Agatston score for subjects with emphysema (> 10%LAA) were 1.88 [1.29–2.76], but this was not significant after adjustment for age and sex.
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Association between baseline Agatston score and prevalent VFs
Characteristics of subjects with (n = 123) or without (n = 463) prevalent VFs are given in Table 3. In univariate models, subjects with medium (2.62 [1.52–4.51]) or high Agatston score (4.51 [2.49–8.16]) had higher odds of having a prevalent VF compared to subjects with a zero Agatston score. In multivariate models, subjects with medium (OR = 1.91 [1.07–3.40]) or high Agatston score (OR = 2.71 [1.40–5.26]) had significantly increased odds of having a prevalent VF compared to subjects with a zero score. After additional adjustment for BA, subjects with high but not medium Agatston score had significantly higher odds of prevalent VFs than subjects with a zero score (OR = 2.03 [1.01–4.08] and OR = 1.60 [0.88–2.92] respectively; Table 3).
Table 3
Association between Agatston score and prevalent VFs
Without VF
With VF
Univariate
Multivariate, without BA
Multivariate, with BA
n = 463
n = 123
OR
95% CI
OR
95% CI
OR
95% CI
Age (years: mean, sd)
59.1
8.3
62.6
7.7
Per + 8 years
1.55
[1.255–1.907]
1.24
[0.983–1.572]
0.97
[0.743–1.255]
Sex (M: n, %)
267
57.7
98
79.7
M vs F
2.88
[1.787–4.633]
2.28
[1.394–3.741]
2.87
[1.707–4.812]
BMI (kg/m2)
26.1
4.3
26.0
4.0
Per + 4 kg/m2
0.97
[0.803–1.169]
–
–
Current smoker (n, %)
224
48.4
51
41.5
Vs former smoker
0.76
[0.505–1.130]
–
–
Pack years (mean, n)
39.6
23.5
46.6
25.3
Per + 24 pack years
1.30
[1.080–1.555]
1.08
[0.886–1.320]
1.09
[0.881–1.351]
COPD (n, %)
317
68.5
94
76.4
Vs no COPD
1.49
[0.942–2.365]
–
–
BA (HU: mean, sd)
165.8
45.2
130.2
40.3
Per − 46 HU
2.60
[2.008–3.375]
–
2.54
[1.899–3.400]
Emphysema (> 10%LAA: n, %)
201
43.4
62
50.4
Vs “no emphysema”
1.32
[0.890–1.973]
–
–
Zero Agatston group (n, %)
176
38.0
20
16.3
–
–
Medium Agatston group (n, %)
205
44.3
61
49.6
Vs zero Agatston score
2.62
[1.520–4.510]
1.91
[1.069–3.396]
1.60
[0.876–2.915]
High Agatston group (n, %)
82
17.7
42
34.1
Vs zero Agatston score
4.51
[2.490–8.159]
2.71
[1.389–5.264]
2.03
[1.013–4.080]
Significant ORs are in italic format. For continuous variables, ORs are given per standard deviation of the total population. Multivariate: confounders included according to > 5% rule (age, sex, and having COPD included in all models)
VF vertebral fracture, OR odds ratio, 95% CI 95% confidence interval, BMI body mass index, COPD chronic obstructive pulmonary disease, BA bone attenuation, HU Hounsfield units, %LAA % low attenuation area
Association between baseline Agatston score and incident VFs within 1 and 3 years
There were 57 subjects (9.7%) with incident VFs within 1 year and 137 (23.4%) within 3 years after baseline (Table 1). Of the subjects with one or more incident VFs within 3 years, 40 (29.2%) had zero Agatston score, 60 (43.8%) had medium Agatston score, and 37 (27%) had high Agatston score (Table 4). In subjects without incident VFs, these numbers were 156 (35%), 206 (46%), and 87 (19%) respectively (Supplemental Figure 2).
Table 4
Association between Agatston score group and incident VFs
Univariate
Multivariate, without BA
Multivariate, with BA
HR
95% CI
HR
95% CI
HR
95% CI
One-year incidence
Age (per + 8 years)
1.49
[1.129–1.954]
1.25
[0.897–1.737]
1.17
[0.827–1.645]
Sex (M vs F)
2.05
[1.107–3.815]
1.43
[0.738–2.749]
1.46
[0.757–2.831]
BMI (per + 4 kg/m2)
0.84
[0.652–1.082]
0.90
[0.690–1.177]
0.96
[0.726–1.269]
Current smoker (vs former)
0.95
[0.561–1.592]
1.28
[0.716–2.296]
1.32
[0.736–2.367]
Pack years (per + 24 pack years)
1.21
[0.977–1.489]
1.07
[0.825–1.379]
1.07
[0.827–1.382]
COPD (vs no COPD)
1.78
[0.925–3.446]
0.86
[0.352–2.077]
0.87
[0.357–2.106]
Emphysema (> 10%LAA, vs ≤ 10%LAA)
2.11
[1.234–3.620]
1.89
[0.936–3.805]
1.85
[0.913–3.740]
BA (per − 46 HU)
1.79
[1.337–2.395]
1.27
[0.910–1.785]
≥ 1 prevalent VF (vs none)
3.93
[2.338–6.607]
3.19
[1.830–5.553]
2.75
[1.523–4.970]
Medium Agatston score (vs zero)
1.16
[0.595–2.272]
0.69
[0.335–1.425]
0.67
[0.323–1.373]
High Agatston score (vs zero)
2.37
[1.206–4.662]
1.05
[0.481–2.279]
0.97
[0.444–2.130]
Three-year incidence
Age (per + 8 years)
1.24
[1.046–1.466]
1.12
[0.916–1.378]
1.05
[0.847–1.297]
Sex (M vs F)
1.70
[1.161–2.484]
1.33
[0.890–1.998]
1.37
[0.915–2.060]
BMI (per + 4 kg/m2)
0.92
[0.784–1.080]
0.97
[0.817–1.152]
1.03
[0.859–1.226]
Current smoker (vs former)
1.11
[0.798–1.558]
1.37
[0.939–2.006]
1.40
[0.956–2.039]
Pack years (per + 24 pack years)
1.09
[0.939–1.274]
0.97
[0.811–1.165]
0.98
[0.816–1.173]
COPD (vs no COPD)
1.40
[0.940–2.075]
1.15
[0.686–1.931]
1.16
[0.690–1.939]
Emphysema (> 10%LAA, vs ≤ 10%LAA)
0.74
[0.526–1.028]
1.24
[0.812–1.891]
1.22
[0.796–1.862]
BA (per − 46 HU)
1.56
[1.302–1.878]
1.29
[1.034–1.598]
≥ 1 prevalent VF (vs none)
3.50
[2.503–4.892]
3.30
[2.307–4.713]
2.84
[1.944–4.152]
Medium Agatston score (vs zero)
1.11
[0.741–1.649]
0.76
[0.494–1.180]
0.73
[0.472–1.129]
High Agatston score (vs zero)
1.46
[0.935–2.287]
0.80
[0.479–1.335]
0.74
[0.441–1.241]
Significant HRs are in italic format. For continuous variables, HRs are given per standard deviation of the total population. Multivariate: confounders included according to > 5% rule (age, sex, and having COPD included in all models)
VF vertebral fracture, HR hazard ratio, 95% CI 95% confidence interval, BMI body mass index, COPD chronic obstructive pulmonary disease, %LAA % low attenuation area, BA bone attenuation, HU Hounsfield units
In univariate models, subjects with high prevalent Agatston score had a significantly increased risk of 1-year VF incidence (HR = 2.37 [1.21–4.66]), but of the higher 3-year incidence risk was not significant (HR = 1.46 [0.94–2.29]). In multivariate models, subjects with medium or high prevalent Agatston score had no significantly increased VFs risk and this was mainly due to the effect of adding prevalent VFs, age, and BA to the model. However, subjects with a prevalent VF and, in case of 3-year incidence, also subjects with lower BA had increased risk of VF incidence.
When the number of incident VFs (0, 1, or ≥ 2) was taken as outcome measure, there were significant univariate associations between subjects with high Agatston score and ≥ 2 incident VFs (1-year OR = 9.72 [2.11–44.72]; 3-year OR = 3.79 [1.64–8.73]), but in multivariate models, this was no longer significant.
Discussion
In this study, we found an association between prevalent VFs and high Agatston score on chest CT scans in (former) smokers with and without COPD. Associations between Agatston score and incident VFs were not significant in multivariate models. The clinical consequence is that when chest CT scans are performed in (former) smokers to evaluate pulmonary function, supplementary evaluation of the presence of VFs and the degree of CAC gives information on risk factors for other diseases for which additional treatment can be considered.
In line with an earlier review [9], we found significant independent associations between BA, prevalent VFs, and high Agatston score. An association between VFs and Agatston score is consistent with the reported correlation of FRAX-estimated risk of hip fracture or major osteoporotic fracture with CAC score [38]. Some linking mechanisms have been proposed, such as an association between micro- and macro-vessel damage and altered microarchitectural indices in the radius [39], or between decreased vascular flow in the lower extremities and increased rate of bone loss at the hip and calcaneus in older women [40]. We suggest that the association between CAC and VFs in part may be explained by the shared risk factor of smoking.
Vascular calcifications in the aorta are commonly seen in patients with osteoporosis, and both increase with aging and with renal disease. Possibly related to renal disease, vascular smooth muscle cells can secrete inhibitors of wnt-signalling that lower bone formation rates. It has been postulated that low bone formation rates fail to buffer high mineral loads and could thus predispose to vascular calcification.
Current smokers had lower odds of having a medium (Table 2; OR = 0.58 [0.40–0.85]) or a high (OR = 0.50 [0.32–0.79]) Agatston score compared to former smokers. This may seem unexpected, but in this population, former smokers were significantly older (62.3 ± 7.1 vs 57.0 ± 8.7), more often had COPD (84.9% vs 53.5%), and had lower BA (154.0 ± 45.3 vs 163.2 ± 47.4) as compared to current smokers.
There were relatively fewer women (n = 221) than men (n = 365) included in this study. A lower percentage of women had a prevalent VF than men in this subcohort (11.3% vs 26.8%), as reported in our previous study (14.1% vs 24.5%) [8]. In addition, women less often had medium (40.7%) or high Agatston score (12.7%) than men (48.2% and 26.3% resp.), resulting in very few women with VF and high Agatston score (n = 6 (3%)).
When analyses regarding the associations between Agatston score and VFs were performed for men and women separately, the associations for men were similar as presented. However, the associations in women were not significant, not surprisingly given the small number of women in the high risk group. However, it is possible that the women included in this study do not represent the female COPD and smoker population (selection bias) and/or that the association between CAC and VFs is different in men and women.
The literature has been contradictory about the association between emphysema and CACS; some studies found an association between emphysema and CAC [12, 13], while others did not [14‐16]. Although we found a univariate association between emphysema and medium or high Agatston score (Table 2), this association was not significant after adjustment for age and sex.
Although there was a significant univariate association between high Agatston score and 1-year incidence, and between high Agatston score and ≥ 2 incident VFs within 1 and 3 years, we did not find any significant multivariate associations between Agatston score and incident VFs after adjusting for age, sex, BA, and prevalent VFs.
A recent meta-analysis examined data from prospective cohort studies of aortic calcification and fractures [41]. Although not all individual studies found significant results, it was reported that subjects in the high aortic calcification category had increased fracture risk compared to subjects in the zero category. However, when types of fractures were specified, Wei et al. reported that the association with incident VFs was not significant, consistent with our findings. They also reported a significant association with incident hip fractures, for which we do not have data. In our results, prevalent VFs (associated with high Agatston score (Tables 2 and 3)) and to a lesser extent baseline BA were significantly associated with incident VFs, also consistent with previous findings [37]. The significant association between high Agatston score and incident VFs disappeared after adjustment. The point estimate shifted towards one which suggests that there is no association between Agatston score measured at baseline and the risk of incident VFs within 1 year. Moreover, there was also no association with incident VFs over 3 years.
In multivariate models, we found an association between prevalent VFs and high Agatston score. Therefore, we suggest all current and former smokers diagnosed with either osteoporosis; a prevalent VF or coronary artery calcification should be evaluated for the osteoporosis or coronary artery disease respectively. Given the relative simplicity of measuring the Agatston score on the chest CTs, we suggest this measurement should be routinely performed and reported and high levels should trigger further assessments including of bone attenuation and coronary artery disease.
There are several limitations in this study.
First, there is a chance of a selection bias due to inclusion of subjects for the ECLIPSE study and selection of subjects from the ECLIPSE study. For the ECLIPSE study, subjects were recruited from outpatient clinics, and subjects with mild COPD (GOLD stage I) or who were using oral GC at baseline were excluded. In addition, only non-Hispanic whites were included. From the ECLIPSE study, only subjects with availability of the complete VF assessment [8] and Agatston score evaluation [15] were included for this study. Subjects included (n = 586) were somewhat younger, had lower BMI, less often had COPD, more often were current smokers, and less often had emphysema (> 10%LAA) compared to all ECLIPSE subjects (n = 2298). For characteristics of included subjects (n = 586) and total ECLIPSE population (n = 2298), see e-Table 1, online supplement.
VF data were only available from vertebrae T1–L1, so information on prevalent or incident VFs in L2–L5 is lacking. Also, due to the design of the ECLIPSE study information on other risk factors for fractures, such as the history of falls and fractures, family history of other fractures (including hip fracture), presence of rheumatoid arthritis, or other metabolic disorders were not available and could not be used for adjustment of models for incident VFs. Lastly, Agatston score was only measured at baseline and follow-up data concerning Agatston score are not available.
Conclusion
In this study of current and former smokers from the ECLIPSE study, we have shown a significant association between prevalent VFs and Agatston score, underlining the concept of multiple comorbidities in this patient group. This indicates that in clinical practice, (former) smokers, who are assessed for and diagnosed with either VF or CAC, should be screened for the other. Since VFs and Agatston score can be diagnosed in CT scans made for pulmonary evaluation, we also recommend opportunistic evaluation of these scans for VFs and CACs as potentially important comorbidities, each of which is amenable to effective interventions.
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Disclaimer
This research was performed independently from funders.
Conflicts of interest
Prof. Geusens reports grants, speaker fees and advisory board from Amgen, grants from Pfizer, grants from MSD, grants from UCB, grants from Abbott, grants and speaker fees from Lilly, grants from BMS, grants from Novartis, grants from Roche, and grants from Will Pharma, outside the submitted work. Prof. Eisman reports grants and honoraria from Eli Lilly, Amgen and Merck, outside the submitted work. Prof. Wouters reports board membership at Boehringer, grants and speaker fees from AstraZeneca, grants and speaker fees from GSK, speaker fees from Novartis, and speaker fees from Chiesi, outside the submitted work. Prof. van den Bergh reports grants from Eli Lilly, grants from Will Pharma, and grants from Amgen, outside the submitted work. The rest of the authors have nothing to disclose.
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