Introduction
Depression is a serious, common mental health condition that incorporates feelings of sadness or hopelessness necessitating clinical intervention. Depression prevalence increases with age and is associated with higher levels of morbidity, suicide, self-neglect and reduced physical, cognitive and social functioning [
1‐
4]. As lifespans increase, identification of those at greater risk of age-related conditions, including depression, becomes increasingly important to ensure appropriate provision of treatment [
5,
6].
The most rapidly expanding age demographic in Northern Ireland (NI) are those aged 50 years (yrs) and above. The NI population provides a unique opportunity for evaluation of depression in a setting historically shaped by conflict and an undertone of sectarian violence [
7,
8]. ‘The Troubles’ was a period of conflict that spanned from 1969 to 1994 and resulted in increased psychological morbidity and mental health problems among individuals exposed to higher levels of conflict [
9‐
11]. During this time, a five-fold increase in anti-depressant prescriptions was reported in the NI population (1989–2000), especially among those aged > 55 yrs. who were three times more likely to be prescribed anti-depressant medication than the 15–24 yrs. demographic [
9], highlighting the increasing burden of depression among the local older population.
The retinal microvasculature shares similar anatomical and physiological features with other end organs including the brain, heart and kidneys, and subtle variations in retinal microvascular parameters (RMPs) may reflect similar variation within the cerebral, renal and coronary circulation [
12‐
14]. Recent advances in retinal fundus imaging provide a unique and non-invasive assessment of the microvasculature currently not possible elsewhere in the body [
15‐
18], enabling opportunistic evaluation of vascular disease and the wider systemic circulation [
13‐
18].
Variation in RMPs have been previously reported in association with cognitive impairment, depression and cerebrovascular disease [
19‐
22] although the nature of associations between RMPs and depression have been inconsistent [
23‐
27]. A ‘vascular depression’ hypothesis has previously implicated cerebrovascular disease in the predisposition, precipitation, and perpetuation of geriatric depressive syndromes [
28]. As such, the aim of this study was to assess associations between RMPs and measures of depression in the Northern Ireland Cohort for the Longitudinal Study of Ageing (NICOLA).
Results
Data were available for 1376 participants of which 113 (8.2%) and 1263 (91.8%) participants were classified with and without depression (Table
1). Participants had a mean age of 62.0 ± 8.4 yrs. and 52% were female, with 8% categorised as smokers (Table
1). The mean CES-D score for all participants was 5.9 ± 6.4. MABP was 98.2 ± 12.6 mmHg, and 23 and 6% of participants were characterised with diabetes and history of CVD respectively. As expected, individuals with depression had a higher CES-D test score than those without (22.0 ± 6.2 versus 4.4 ± 3.9, respectively). Those classified as free from depression had a higher mean age (62.2 ± 8.4 yrs. versus 59.3 ± 8.1 yrs), a lower percentage were female (52% versus 59%) and they had a lower mean BMI (28.3 ± 4.7 kg/m
2 versus 29.6 ± 5.4 kg/m
2) compared to those with depression. Participants with depression were more likely to smoke (12% versus 8%), and a higher proportion had diabetes (29% versus 23%).
Table 1
Participant summary characteristics
Mean age (years, SD) | 62.0 ± 8.4 | 62.2 ± 8.4 | 59.3 ± 8.1 | < 0.01 |
Female, n (%) | 717 (52.1) | 650 (51.5) | 67 (59.3) | 0.11 |
Smoking status, yes n (%) | 115 (8.4) | 101 (8.0) | 14 (12.4) | 0.11 |
Alcohol consumption, non-drinker, n (%) | 280 (20.3) | 259 (20.5) | 21 (18.6) | 0.26 |
Education, secondary and above, n (%) | 1228 (89.2) | 1124 (89.0) | 104 (92.0) | 0.32 |
Diabetes, yes n (%) | 317 (23.0) | 284 (22.5) | 33 (29.2) | 0.10 |
Mean BMI (kg/m2, SD) | 28.4 ± 4.8 | 28.3 ± 4.7 | 29.6 ± 5.4 | < 0.01 |
Mean arterial blood pressure (mm Hg, SD) | 98.2 ± 12.6 | 98.3 ± 12.6 | 96.8 ± 12.8 | 0.24 |
Cardiovascular disease, no n (%) | 1292 (93.9) | 1187 (94.0) | 105 (92.9) | 0.65 |
Mean triglyceride (mmol/L, SD) | 1.6 ± 0.9 | 1.6 ± 0.9 | 1.6 ± 0.8 | 0.50 |
Mean HDL cholesterol (mmol/L, SD) | 1.6 ± 0.5 | 1.6 ± 0.5 | 1.6 ± 0.4 | 0.88 |
Mean LDL cholesterol (mmol/L, SD) | 3.5 ± 1.1 | 3.4 ± 1.1 | 3.5 ± 1.1 | 0.56 |
Mean CES-D score (SD) | 5.9 ± 6.4 | 4.4 ± 3.9 | 22.0 ± 6.2 | < 0.01 |
Left and right eye CRAE and CRVE comparisons from 75 participants were not significantly different (P
Crae = 0.08; P
Crve = 0.89). Only mean arteriolar tortuosity was significantly lower in those with depression in unadjusted comparisons (0.085 versus 0.113;
P = 0.03; Table
2). Decreased retinal arteriolar tortuosity was associated with depression before and after adjustment for potential confounding variables across all regression models (unadjusted: odds ratio [OR] = 0.81; 95% confidence interval [CI]: 0.67, 0.98; P = 0.03, minimally adjusted: OR = 0.81; 95% CI: 0.67, 0.98; P = 0.03; and fully adjusted: OR = 0.79; 95% CI: 0.65, 0.96;
P = 0.02); Table
2 and Table
3). No additional significant associations between RMPs and depression were detected (Table
3). In a sensitivity analysis that excluded the 317 participants with diabetes, the effect size of the association observed between arteriolar tortuosity and depression was slightly attenuated and no longer significant given the reduced sample size (Supplementary Table
2).
Table 2
Summary of participant retinal microvascular parameters
Mean CRAE (PX, SD) | 29.613 ± 2.186 | 29.636 ± 2.190 | 29.354 ± 2.136 | 0.19 |
Mean CRVE (PX, SD) | 40.849 ± 3.314 | 40.857 ± 3.298 | 40.757 ± 3.501 | 0.76 |
Mean AVR (SD) | 0.728 ± 0.062 | 0.728 ± 0.061 | 0.724 ± 0.066 | 0.47 |
Mean fractal dimension arteriolar (SD) | 1.557 ± 0.052 | 1.557 ± 0.053 | 1.563 ± 0.046 | 0.18 |
Mean fractal dimension venular (SD) | 1.539 ± 0.051 | 1.539 ± 0.051 | 1.540 ± 0.047 | 0.89 |
Mean tortuosity arteriolar (SD) | 0.111 ± 0.149 | 0.113 ± 0.153 | 0.085 ± 0.090 | 0.03 |
Mean tortuosity venular (SD) | 0.068 ± 0.109 | 0.069 ± 0.111 | 0.063 ± 0.075 | 0.38 |
Table 3
Logistic regression analysis of retinal microvascular parameters and depression
aCRAE (PX) | 0.88 | 0.72, 1.08 | 0.21 | 0.89 | 0.73, 1.09 | 0.27 | 0.86 | 0.70, 1.06 | 0.17 |
aCRVE (PX) | 0.99 | 0.82, 1.20 | 0.92 | 0.98 | 0.81, 1.19 | 0.85 | 0.97 | 0.79, 1.18 | 0.75 |
aAVR | 0.92 | 0.75, 1.12 | 0.40 | 0.94 | 0.77, 1.15 | 0.52 | 0.92 | 0.75, 1.13 | 0.45 |
aFractal dimension arteriolar | 1.12 | 0.91, 1.37 | 0.28 | 1.13 | 0.92, 1.38 | 0.24 | 1.12 | 0.91, 1.37 | 0.30 |
aFractal dimension venular | 1.00 | 0.83, 1.20 | 0.97 | 1.00 | 0.82, 1.20 | 0.96 | 0.99 | 0.82, 1.19 | 0.89 |
abTortuosity arteriolar | 0.81 | 0.67, 0.98 | 0.03 | 0.80 | 0.66, 0.97 | 0.02 | 0.79 | 0.65, 0.96 | 0.02 |
abTortuosity venular | 0.93 | 0.76, 1.13 | 0.45 | 0.90 | 0.74, 1.11 | 0.33 | 0.91 | 0.74, 1.12 | 0.36 |
Discussion
We detected significantly lower levels of retinal arteriolar tortuosity, a complexity measure of the twisting and turning of the retinal microvasculature [
32], associated with depression independent of potential confounders in older adults. Depression is a condition that affects a significant number of older people, many with underlying chronic illness or cognitive impairment, which leads to disruption of daily life and increased levels of morbidity and mortality. Age and disease related processes, including arteriosclerosis and inflammatory, endocrine, and immune changes, have been associated with the disease processes that characterises depression, especially in those of advanced age [
33].
Alexopoulos and colleagues proposed a ‘vascular depression’ hypothesis implicating cerebrovascular disease in the predisposition, precipitation, and perpetuation of geriatric depressive syndromes [
28], which provided a rationale for the investigation of associations between RMPs and depression [
34]. Previous studies have reported associations between severe and transitory depression and vascular endothelial dysfunction [
35‐
38] but to our knowledge, the current study is the first to report associations between decreased arteriolar tortuosity and later-life depression. Endothelial cells also play a key role in regulating retinal microvascular blood flow and angiogenesis and our findings may implicate microvascular endothelial dysfunction and reduced retinal arteriolar tortuosity and in older people with depression independent of potential confounding factors. Indeed, a recent systematic-review and meta-analyses reported associations between peripheral and cerebral forms of microvascular dysfunction with an increased odds of incident late-life depression supporting the hypothesis that microvascular dysfunction is causally linked to depression and a potential target for the prevention and treatment of symptoms [
39]. The meta-analysis by van Agtmaal and colleagues evaluated several outcome measures including retinal vessel calibre [
23,
40], detecting no association with depression, in line with the findings from our study.
Technological advances in non-invasive retinal image acquisition and analysis have enabled more accurate quantification of microvascular health [
17]. A small number of studies investigating associations between RMPs and depression have reported inconsistent findings [
23‐
27]. Furthermore, these studies were largely limited to the assessment of retinal vessel calibre only and were unable to consider other RMPs such as fractal dimension or tortuosity, coefficients that reflect the status of microvascular health. Sun and colleagues reported no significant associations between retinal microvascular calibre and depression similarly characterised using the CES-D questionnaire in 2420 individuals ≥65 yrs. old in the population-based Cardiovascular Health Study [
26]. The Rotterdam population-based study also characterised depression using either the CES-D questionnaire or the Hospital Anxiety and Depression Scale, and reported no significant associations between retinal microvascular calibre and late-life depression in 3605 participants ≥55 yrs. [
23]. Kim and colleagues identified retinal arteriolar narrowing in individuals characterised with depression using the CES-D questionnaire in a cross-sectional study of 1744 older adults (mean age of 78 yrs) from the Cardiovascular Health Study [
27]. Other forms of depression and age categories have also been considered. Li and colleagues measured symptoms of antenatal anxiety and depression using the State-Trait Anxiety Inventory and Edinburgh Postnatal Depression Scale reporting associations between wider retinal arterioles and depression in 952 pregnant women [
25]. Wider retinal arterioles were also reported by Meier and colleagues in 865 adolescents and young adults from the Brisbane Longitudinal Twin Study and the Twin Eye Study with symptoms of anxiety characterised by the Somatic and Psychological Health Report questionnaire, with the authors suggesting this may implicate microvascular variation as an early mechanistic factor in depression disease aetiology [
24], in contrast to arteriolar narrowing described by Kim and colleagues in older adults [
24,
27].
Our study had several limitations. Firstly, the study population consisted of Caucasian participants, aged > 50 yrs. and may represent the ‘worried-well’, limiting the generalisability of our results. Secondly, although NICOLA is a longitudinal study, only baseline data were available for this cross-sectional analysis precluding assumptions regarding causality. Thirdly, although adjustment for a large number of potential confounders was made, the possibility of residual confounding remains. Additionally, despite adjusting for cardiometabolic, atherosclerotic and diabetic risk factors, we cannot fully discount their potential influence on our findings, given previously reported associations [
41‐
45].
Despite the limitations, our study had several strengths. Similar to previous investigations of RMPs and depression [
23‐
27], optic disc centred fundus images provided a more accurate quantification of RMPs compared to macula centred images, which generally limit assessment to the temporal retinal arcades. Although our analysis was cross-sectional in design, it provides a rationale for further evaluation of RMPs in future waves of NICOLA data. If clinical value is associated with identifying those individuals at increased risk of depression by using RMPs, earlier identification of older individuals at increased risk of adverse vascular events associated with depression may be possible. In this study, retinal images from the left eye were analysed except when unavailable or of insufficient quality, in which case the right eye image was used. This was unlikely to have limited the study conclusions, as comparable investigations have previously reported high correlations between RMPs from right and left eye comparisons [
46‐
48], similar to the comparisons undertaken in a subset of NICOLA participants.
Furthermore, the well-characterised population-based study design added validity to our findings. Finally, the CES-D questionaire to classify depression is well accepted with previously reported sensitivity and specificity values of 0.87 and 0.70, respectivly [
49] and the exclusion of participants using anti-depressive medications or with MCI improved the characterisation of depression, given those with depressive symptoms are more likely to score poorly on tests of cognitive function [
50].
In conclusion, we report decreased retinal arteriolar tortuosity in association with depression in an older population independent of potential confounding factors. These retinal measures may provide non-invasive assessment of microvascular complications associated with depression.
Acknowledgments
We are grateful to all the participants of the NICOLA Study, and the whole NICOLA team, which includes nursing staff, research scientists, clerical staff, computer and laboratory technicians, managers and receptionists. The Atlantic Philanthropies, the Economic and Social Research Council, the UKCRC Centre of Excellence for Public Health Northern Ireland, the Centre for Ageing Research and Development in Ireland, the Office of the First Minister and Deputy First Minister, the Health and Social Care Research and Development Division of the Public Health Agency, the Wellcome Trust/Wolfson Foundation and Queen’s University Belfast provide core financial support for NICOLA. The authors alone are responsible for the interpretation of the data and any views or opinions presented are solely those of the authors and do not necessarily represent those of the NICOLA Study team.
Rachael O’Neill was supported by a PhD studentship from the Department for Economy, Northern Ireland. This work was supported by the following funders who provide core financial support for the NICOLA Study: the Atlantic Philanthropies; the Economic and Social Research Council; the UKCRC Centre of Excellence for Public Health Northern Ireland; the Centre for Ageing Research and Development in Ireland; the Office of the First Minister and Deputy First Minister; the Health and Social Care Research and Development Division of the Public Health Agency; the Wellcome Trust/Wolfson Foundation; and Queen’s University Belfast.
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