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Erschienen in: International Orthopaedics 8/2014

01.08.2014 | Original Paper

Bacterial adherence to different components of total hip prosthesis in patients with prosthetic joint infection

verfasst von: Richard Lass, Alexander Giurea, Bernd Kubista, Alexander M. Hirschl, Wolfgang Graninger, Elisabeth Presterl, Reinhard Windhager, Johannes Holinka

Erschienen in: International Orthopaedics | Ausgabe 8/2014

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Abstract

Purpose

The purpose of our study was to evaluate and quantify the bacterial adherence to the different components of total hip prosthesis.

Methods

The bacterial load of 80 retrieved hip components from 24 patients was evaluated by counting of colony-forming units (CFU) dislodged from component surfaces using the sonication culture method.

Results

Micro-organisms were detected in 68 of 80 explanted components. The highest bacterial load was detected on the polyethylene liners, showing a significant difference in distribution of CFU between the liner and metal components (stem and cup). Staphylococcus epidermidis was identified as the pathogen causing the highest CFU count, especially from the polyethylene liner.

Conclusions

Results of our study confirm that sonicate culture of the retrieved liners and heads, which revealed the highest bacterial loads, are reliable and sufficient for pathogen detection in the clinical diagnostic routine.
Literatur
2.
Zurück zum Zitat Charnley J, Eftekhar N (1969) Postoperative infection in total prosthetic replacement arthroplasty of the hip-joint. With special reference to the bacterial content of the air of the operating room. Br J Surg 56:641–649PubMedCrossRef Charnley J, Eftekhar N (1969) Postoperative infection in total prosthetic replacement arthroplasty of the hip-joint. With special reference to the bacterial content of the air of the operating room. Br J Surg 56:641–649PubMedCrossRef
3.
Zurück zum Zitat Lidwell OM, Elson RA, Lowbury EJ, Whyte W, Blowers R, Stanley SJ, Lowe D (1987) Ultraclean air and antibiotics for prevention of postoperative infection. A multicenter study of 8,052 joint replacement operations. Acta Orthop Scand 58:4–13PubMedCrossRef Lidwell OM, Elson RA, Lowbury EJ, Whyte W, Blowers R, Stanley SJ, Lowe D (1987) Ultraclean air and antibiotics for prevention of postoperative infection. A multicenter study of 8,052 joint replacement operations. Acta Orthop Scand 58:4–13PubMedCrossRef
4.
Zurück zum Zitat Lidwell OM, Lowbury EJ, Whyte W, Blowers R, Stanley SJ, Lowe D (1982) Effect of ultraclean air in operating rooms on deep sepsis in the joint after total hip or knee replacement: a randomised study. Br Med J 285:10–14CrossRef Lidwell OM, Lowbury EJ, Whyte W, Blowers R, Stanley SJ, Lowe D (1982) Effect of ultraclean air in operating rooms on deep sepsis in the joint after total hip or knee replacement: a randomised study. Br Med J 285:10–14CrossRef
6.
Zurück zum Zitat Phillips JE, Crane TP, Noy M, Elliott TS, Grimer RJ (2006) The incidence of deep prosthetic infections in a specialist orthopaedic hospital: a 15-year prospective survey. J Bone Joint Surg (Br) 88:943–948. doi:10.1302/0301-620X.88B7.17150 CrossRef Phillips JE, Crane TP, Noy M, Elliott TS, Grimer RJ (2006) The incidence of deep prosthetic infections in a specialist orthopaedic hospital: a 15-year prospective survey. J Bone Joint Surg (Br) 88:943–948. doi:10.​1302/​0301-620X.​88B7.​17150 CrossRef
8.
Zurück zum Zitat Tunney MM, Patrick S, Curran MD, Ramage G, Hanna D, Nixon JR, Gorman SP, Davis RI, Anderson N (1999) Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol 37:3281–3290PubMedCentralPubMed Tunney MM, Patrick S, Curran MD, Ramage G, Hanna D, Nixon JR, Gorman SP, Davis RI, Anderson N (1999) Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol 37:3281–3290PubMedCentralPubMed
9.
Zurück zum Zitat Costerton JW (2005) Biofilm theory can guide the treatment of device-related orthopaedic infections. Clin Orthop Relat Res 7–11 Costerton JW (2005) Biofilm theory can guide the treatment of device-related orthopaedic infections. Clin Orthop Relat Res 7–11
11.
Zurück zum Zitat Trampuz A, Piper KE, Jacobson MJ, Hanssen AD, Unni KK, Osmon DR, Mandrekar JN, Cockerill FR, Steckelberg JM, Greenleaf JF, Patel R (2007) Sonication of removed hip and knee prostheses for diagnosis of infection. N Engl J Med 357:654–663. doi:10.1056/NEJMoa061588 PubMedCrossRef Trampuz A, Piper KE, Jacobson MJ, Hanssen AD, Unni KK, Osmon DR, Mandrekar JN, Cockerill FR, Steckelberg JM, Greenleaf JF, Patel R (2007) Sonication of removed hip and knee prostheses for diagnosis of infection. N Engl J Med 357:654–663. doi:10.​1056/​NEJMoa061588 PubMedCrossRef
12.
Zurück zum Zitat Esteban J, Gomez-Barrena E, Cordero J, Martin-de-Hijas NZ, Kinnari TJ, Fernandez-Roblas R (2008) Evaluation of quantitative analysis of cultures from sonicated retrieved orthopedic implants in diagnosis of orthopedic infection. J Clin Microbiol 46:488–492. doi:10.1128/JCM.01762-07 PubMedCentralPubMedCrossRef Esteban J, Gomez-Barrena E, Cordero J, Martin-de-Hijas NZ, Kinnari TJ, Fernandez-Roblas R (2008) Evaluation of quantitative analysis of cultures from sonicated retrieved orthopedic implants in diagnosis of orthopedic infection. J Clin Microbiol 46:488–492. doi:10.​1128/​JCM.​01762-07 PubMedCentralPubMedCrossRef
13.
Zurück zum Zitat Holinka J, Bauer L, Hirschl AM, Graninger W, Windhager R, Presterl E (2011) Sonication cultures of explanted components as an add-on test to routinely conducted microbiological diagnostics improve pathogen detection. J Orthop Res 29:617–622. doi:10.1002/jor.21286 PubMedCrossRef Holinka J, Bauer L, Hirschl AM, Graninger W, Windhager R, Presterl E (2011) Sonication cultures of explanted components as an add-on test to routinely conducted microbiological diagnostics improve pathogen detection. J Orthop Res 29:617–622. doi:10.​1002/​jor.​21286 PubMedCrossRef
14.
Zurück zum Zitat Janz V, Wassilew GI, Hasart O, Matziolis G, Tohtz S, Perka C (2013) Evaluation of sonicate fluid cultures in comparison to histological analysis of the periprosthetic membrane for the detection of periprosthetic joint infection. Int Orthop 37:931–936. doi:10.1007/s00264-013-1853-1 PubMedCentralPubMedCrossRef Janz V, Wassilew GI, Hasart O, Matziolis G, Tohtz S, Perka C (2013) Evaluation of sonicate fluid cultures in comparison to histological analysis of the periprosthetic membrane for the detection of periprosthetic joint infection. Int Orthop 37:931–936. doi:10.​1007/​s00264-013-1853-1 PubMedCentralPubMedCrossRef
15.
Zurück zum Zitat Petty W, Spanier S, Shuster JJ, Silverthorne C (1985) The influence of skeletal implants on incidence of infection. Experiments in a canine model. J Bone Joint Surg Am 67:1236–1244PubMed Petty W, Spanier S, Shuster JJ, Silverthorne C (1985) The influence of skeletal implants on incidence of infection. Experiments in a canine model. J Bone Joint Surg Am 67:1236–1244PubMed
16.
Zurück zum Zitat Cordero J, Munuera L, Folgueira MD (1996) The influence of the chemical composition and surface of the implant on infection. Injury 27(Suppl 3):SC34–SC37PubMed Cordero J, Munuera L, Folgueira MD (1996) The influence of the chemical composition and surface of the implant on infection. Injury 27(Suppl 3):SC34–SC37PubMed
17.
Zurück zum Zitat Cordero J, Munuera L, Folgueira MD (1994) Influence of metal implants on infection. An experimental study in rabbits. J Bone Joint Surg (Br) 76:717–720 Cordero J, Munuera L, Folgueira MD (1994) Influence of metal implants on infection. An experimental study in rabbits. J Bone Joint Surg (Br) 76:717–720
18.
Zurück zum Zitat Holinka J, Pilz M, Hirschl AM, Graninger W, Windhager R, Presterl E (2012) Differential bacterial load on components of total knee prosthesis in patients with prosthetic joint infection. Int J Artif Organs 35:735–741. doi:10.5301/ijao.5000152 PubMed Holinka J, Pilz M, Hirschl AM, Graninger W, Windhager R, Presterl E (2012) Differential bacterial load on components of total knee prosthesis in patients with prosthetic joint infection. Int J Artif Organs 35:735–741. doi:10.​5301/​ijao.​5000152 PubMed
19.
Zurück zum Zitat Berbari EF, Hanssen AD, Duffy MC, Steckelberg JM, Ilstrup DM, Harmsen WS, Osmon DR (1998) Risk factors for prosthetic joint infection: case–control study. Clin Infect Dis 27:1247–1254PubMedCrossRef Berbari EF, Hanssen AD, Duffy MC, Steckelberg JM, Ilstrup DM, Harmsen WS, Osmon DR (1998) Risk factors for prosthetic joint infection: case–control study. Clin Infect Dis 27:1247–1254PubMedCrossRef
21.
Zurück zum Zitat Gomez-Barrena E, Esteban J, Medel F, Molina-Manso D, Ortiz-Perez A, Cordero-Ampuero J, Puertolas JA (2012) Bacterial adherence to separated modular components in joint prosthesis: a clinical study. J Orthop Res 30:1634–1639. doi:10.1002/jor.22114 PubMedCrossRef Gomez-Barrena E, Esteban J, Medel F, Molina-Manso D, Ortiz-Perez A, Cordero-Ampuero J, Puertolas JA (2012) Bacterial adherence to separated modular components in joint prosthesis: a clinical study. J Orthop Res 30:1634–1639. doi:10.​1002/​jor.​22114 PubMedCrossRef
23.
Zurück zum Zitat Janz V, Wassilew GI, Hasart O, Tohtz S, Perka C (2013) Improvement in the detection rate of PJI in total hip arthroplasty through multiple sonicate fluid cultures. J Orthop Res 31:2021–2024. doi:10.1002/jor.22451 PubMedCrossRef Janz V, Wassilew GI, Hasart O, Tohtz S, Perka C (2013) Improvement in the detection rate of PJI in total hip arthroplasty through multiple sonicate fluid cultures. J Orthop Res 31:2021–2024. doi:10.​1002/​jor.​22451 PubMedCrossRef
Metadaten
Titel
Bacterial adherence to different components of total hip prosthesis in patients with prosthetic joint infection
verfasst von
Richard Lass
Alexander Giurea
Bernd Kubista
Alexander M. Hirschl
Wolfgang Graninger
Elisabeth Presterl
Reinhard Windhager
Johannes Holinka
Publikationsdatum
01.08.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
International Orthopaedics / Ausgabe 8/2014
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-014-2358-2

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