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01.09.2006 | Article

Beta cell compensation for insulin resistance in Zucker fatty rats: increased lipolysis and fatty acid signalling

verfasst von: C. J. Nolan, J. L. Leahy, V. Delghingaro-Augusto, J. Moibi, K. Soni, M.-L. Peyot, M. Fortier, C. Guay, J. Lamontagne, A. Barbeau, E. Przybytkowski, E. Joly, P. Masiello, S. Wang, G. A. Mitchell, M. Prentki

Erschienen in: Diabetologia | Ausgabe 9/2006

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Abstract

Aims/hypothesis

The aim of this study was to determine the role of fatty acid signalling in islet beta cell compensation for insulin resistance in the Zucker fatty fa/fa (ZF) rat, a genetic model of severe obesity, hyperlipidaemia and insulin resistance that does not develop diabetes.

Materials and methods

NEFA augmentation of insulin secretion and fatty acid metabolism were studied in isolated islets from ZF and Zucker lean (ZL) control rats.

Results

Exogenous palmitate markedly potentiated glucose-stimulated insulin secretion (GSIS) in ZF islets, allowing robust secretion at physiological glucose levels (5–8 mmol/l). Exogenous palmitate also synergised with glucagon-like peptide-1 and the cyclic AMP-raising agent forskolin to enhance GSIS in ZF islets only. In assessing islet fatty acid metabolism, we found increased glucose-responsive palmitate esterification and lipolysis processes in ZF islets, suggestive of enhanced triglyceride–fatty acid cycling. Interruption of glucose-stimulated lipolysis by the lipase inhibitor Orlistat (tetrahydrolipstatin) blunted palmitate-augmented GSIS in ZF islets. Fatty acid oxidation was also higher at intermediate glucose levels in ZF islets and steatotic triglyceride accumulation was absent.

Conclusions/interpretation

The results highlight the potential importance of NEFA and glucoincretin enhancement of insulin secretion in beta cell compensation for insulin resistance. We propose that coordinated glucose-responsive fatty acid esterification and lipolysis processes, suggestive of triglyceride–fatty acid cycling, play a role in the coupling mechanisms of glucose-induced insulin secretion as well as in beta cell compensation and the hypersecretion of insulin in obesity.

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Titel: Beta cell compensation for insulin resistance in Zucker fatty rats: increased lipolysis and fatty acid signalling
verfasst von: C. J. Nolan
J. L. Leahy
V. Delghingaro-Augusto
J. Moibi
K. Soni
M.-L. Peyot
M. Fortier
C. Guay
J. Lamontagne
A. Barbeau
E. Przybytkowski
E. Joly
P. Masiello
S. Wang
G. A. Mitchell
M. Prentki
Publikationsdatum: 01.09.2006
Verlag: Springer-Verlag
Erschienen in: Diabetologia / Ausgabe 9/2006
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-006-0305-5

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Abstract

Aims/hypothesis

Materials and methods

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