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07.07.2018 | Original Article

Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars

verfasst von: Tiago Silva da Fonseca, Guilherme F. Silva, Juliane M. Guerreiro-Tanomaru, Mateus Machado Delfino, Estela Sasso-Cerri, Mário Tanomaru-Filho, Paulo Sérgio Cerri

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2019

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Abstract

Objectives

Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA).

Materials and methods

The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey’s test (p ≤ 0.05).

Results

At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups.

Conclusions

Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues.

Clinical relevance

Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.

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Titel: Biodentine and MTA modulate immunoinflammatory response favoring bone formation in sealing of furcation perforations in rat molars
verfasst von: Tiago Silva da Fonseca
Guilherme F. Silva
Juliane M. Guerreiro-Tanomaru
Mateus Machado Delfino
Estela Sasso-Cerri
Mário Tanomaru-Filho
Paulo Sérgio Cerri
Publikationsdatum: 07.07.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2550-7

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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