Background
Lung cancer remains by far the most common cause of cancer mortality, which ranks among the most deadly cancers worldwide [
1]. A growing number of evidences confirm that the tumor microenvironment contains diverse cell populations that interact with cancer cells and participate in all stages of tumorigenesis [
2]. Tumor-infiltrating immune cells and immune responses within the tumor microenvironment are promising therapeutic targets [
3]. Immune evasion represents a hallmark of cancer [
4]. The majority of cancer immunotherapies, including immune checkpoint blockade therapy, aim to counteract immune evasion by shifting the balance in favor of immune activation, enabling T cell-mediated cancer cell elimination [
4,
5]. However, only a subset of patients benefit from immunotherapies, emphasizing the need to identify the genomic and molecular determinants underpinning immune evasion [
6]. Therefore, the discovery of new diagnostic biomarkers and a better understanding of molecular mechanisms underlying the tumor microenvironment of lung cancer are crucial.
During lung cancer, the tumor microenvironment consists of a heterogeneous collection of extracellular matrix, fibroblasts, perivascular cells, and, notably, is enriched in highly active immune cells [
7]. Recent evidence suggests that exosomes are a vital communication medium between different cell types in the tumor microenvironment [
8,
9]. Exosomes, as a novel mechanism of intercellular communication, can shuttle bioactive molecules from one cell to another, leading to the exchange of genetic information and reprogramming of recipient cells [
10,
11]. Increasing evidence suggests that tumor cells release excessive amount of exosomes that promote tumor growth [
10,
11]. In addition, tumor-derived exosomes signal immune cells in tumor microenvironment, helping tumor cells escape immune surveillance [
12]. Accumulating evidence demonstrates that macrophages, the most abundant leukocyte population in lung cancer, have a critical role at each stage of cancer progression [
13]. Such tumor-associated macrophages (TAMs) facilitate neoplastic transformation, tumor immune evasion and the subsequent metastatic cascade [
13]. Thus, understanding the role of exosomes in the regulation of the TAMs response in cancer patients is important because TAMs are the major effector cells mediating anti-tumor immunity.
Tripartite motif (TRIM) family proteins, most of which have E3 ubiquitin ligase activities, have various functions in cellular processes including intracellular signaling, development, apoptosis, protein quality control, innate immunity, autophagy, and carcinogenesis [
14]. The ubiquitin system is one of the systems for post-translational modifications, which play crucial roles not only as markers for degradation of target proteins by the proteasome but also as regulators of protein–protein interactions and of the activation of enzymes. Accumulating evidence has shown that TRIM family proteins have unique, important roles and that their dysregulation causes several diseases classified as cancer, immunological disease, or developmental disorders [
14]. TRIM59 is a member of the TRIM protein superfamily, and has a TRIM or RBCC motif consisting of a RING-finger domain (R), a B-box domain (B), and a coiled-coil domain (CC). Increasing evidence showed that TRIM59 has been identified as contributing to tumor progression [
15‐
18]. Our previous study has demonstrated that TRIM59 upregulated in lung cancer, is required for cancer cells survival and metastasis [
19]. With the unraveling of the relationship between TRIM59 and tumors, TRIM59 is now being recognized as potential therapeutic targets for cancer. However, the mechanisms of TRIM59 promoting cancer metastasis and the communication between the tumor microenvironment and tumor cells are still waiting for exposure.
In this study, our findings unveil a new sight between tumor cells and macrophages: TRIM59 are directly shuttled from cancer cells via exosomes to macrophages, promoting ABHD5 proteasomal degradation and inducing NLRP3 (NLR family protein containing a pyrin domain 3) inflammasome activation and promoting IL-1β secretion, in turn promoting cancer cells proliferation and invasion. Thus, the data implicate TRIM59 as a target for exosome-mediated tumor immune evasion.
Materials and methods
Cell culture
The human cell lines H1299, A549, THP-1, lewis lung carcinoma, and HEK293 were purchased from the Chinese Academy of Science Cell Bank (Shanghai, China). They have been authenticated by a STR DNA profiling analysis and routinely examined for mycoplasma contamination. The cell lines were maintained at 37 °C in a humidified atmosphere of 5% CO2 with DMEM medium containing 10% fetal bovine serum (FBS) with 100 U/ml penicillin G and 100 μg/ml streptomycin sulfate. Bone marrow derived macrophages (BMDMs) were obtained as previously described [
20]. To induce differentiation into macrophages, THP-1 cells (1 × 10
6) were incubated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for 24–48 h.
Exosome isolation and identification
For exosomes isolation, we first transplanted equal number of different cells into 10 cm plates and changed the culture medium with fresh DMEM-supplemented serum, which was depleted of exosomes by centrifugated at 12,000×g overnight. After 48 h, conditioned medium (CM) was collected and filtrated through 0.22 μm filters (Millipore). Exosomes in CM were isolated by a precipitation method using Total Exosome Isolation Reagent (Invitrogen, Cat: #4478359) according to the manufacturer’s instruction. Briefly, Transfer the required volume of cell-free culture media to a new tube and add 0.5 volumes of the Total Exosome Isolation (from cell culture media) reagent. Mix the culture media/reagent mixture well by vortexing. Incubate samples at 2 °C to 8 °C overnight. After incubation, centrifuge the samples at 10,000×g for 1 h at 2 °C to 8 °C. Aspirate and discard the supernatant. Exosomes are contained in the pellet at the bottom of the tube. Resuspend the pellet in a convenient volume of 1X PBS.
Isolated exosomes were mixed with 4% paraformaldehyde. Exosomes were then dropped onto formvar carbon-coated electron microscopy grids and fixed with 1% glutaraldehyde for 10 min. Samples were negatively stained with 2% uranyl acetate solution. Images were obtained using a transmission electron microscopy.
Exosome labeling and tracing
Purified exosomes isolated from the culture medium were collected and labeled with PKH67 Green Fluorescent membrane linker dye (Sigma-Aldrich) according to manufacturer’s instructions. Then, the labeled exosome pellets were resuspended and added to the unstained macrophages for exosomes uptake studies. After incubation for 0 h, 4 h, 8 h or 12 h at 37 °C, cells were observed by fluorescence microscopy.
Western blotting
Exosomes or cells were lysed with RIPA buffer containing a complete protease inhibitor tablet (Roche). Proteins were separated by SDS-PAGE gel and transferred onto polyvinylidene difluoride membranes. After blocked in 5% skim milk for 30 min, membranes were probed with various primary antibodies overnight at 4 °C, followed by incubation with horseradish peroxidase–linked secondary antibodies for 1 h at room temperature, and visualized with electrochemiluminescence by the chemiluminescence instrument. The following antibodies were used: TRIM59 (Invitrogen, Cat: PA5–38726), ABHD5 (Invitrogen, Cat: PA5–78704), CD63 (Invitrogen, Cat: 10628D), CD81 (Invitrogen, Cat: 16–0811-82), TSG101 (Invitrogen, Cat: PA5–82236), SLC16A4(MCT4) (Invitrogen, Cat: PA5–80008), β-actin (Cell Signaling Technology, Cat: #3700), HA-Tag (Cell Signaling Technology, Cat: #3724), Myc-Tag (Cell Signaling Technology, Cat: #2276), IL-1β (Cell Signaling Technology, Cat:#12703), Cleaved-IL-1β (Cell Signaling Technology, Cat: #83186), Caspase-1(Cell Signaling Technology, Cat: #3866), and Cleaved Caspase-1 (Cell Signaling Technology, Cat: #89332).
RNA extraction and qRT-PCR
Total RNA was extracted from cells using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. Aliquots of 1 μg of total RNA were reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen) and oligo-dT (18)-primers (Invitrogen). The real time PCR reaction was performed using SYBR Green Master Mix kit in ABI Prism 7000 Sequence Detection System (Applied Biosystems) according to the manufacturer’s instructions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was amplified as an internal standard. The Taqman probe for IL-1β (Cat: HP100210) and GAPDH (Cat: HP100003) were purchased from Sino Biological Inc. (Beijing, China). The primers for mouse TRIM59: 5′-ATGCACAATTTTGAGGAGGAG-3′ (forward) and 5′-TCAACGAGAAACTATTTTCC-3′ (reverse). The expression levels of the mRNAs were reported as fold changes vs. control.
Immunofluorescence (IF) staining
THP-1 macrophages, TAMs, and BMDMs used in the tests was seeded on cover slides in 24-well plates and incubated overnight. Cells were fixed in 4% paraformaldehyde for 30 min, permeabilized with 1% Triton X-100 for 20 min, blocked in 5% bovine serum albumin for 60 min, and incubated with primary antibodies against TRIM59 and ABHD5 overnight at 4 °C, followed by an Alexa Fluor 594-conjugated secondary antibody and Alexa Fluor 488-conjugated secondary antibody for 30 min at RT. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) at room temperature for 5 min. IF signals were captured using a laser confocal microscopy.
Plasmid constructs and RNA interference
HA-TRIM59 (Cat: HG25849-NY), TRIM59 (Cat:HG25849-UT), Myc-ABHD5 (Cat: HG14216-CM), and ABHD5 (Cat: HG14216-UT) expression plasmid were purchased from Sino Biological Inc. Stable TRIM59 was up-regulated using Human TRIM59 lentiviral particles with C terminal GFP Spark tag (Sino Biological Inc.; Cat: HG25849-ACGLN) as the manufacturer’s instructions.
Scrambled, human ABHD5 short hairpin RNA (shRNA) were obtained from Shanghai Genechem Co., Ltd. (Shanghai, China) and the following target sequences: CCGGGCAGCGTTTAAGGCCTGATTTCTCGAGAAATCAGGCCTTAAACGCTGCTTTTTG. TRIM59 shRNA was described previously [
19]. To elucidate the role of TRIM59 as an E3 ligase, we generated an E3 ligase-defective TRIM59 by using the KOD-Plus-Mutagenesis kit (Toyobo, cat: SMK-101) and verified by performing DNA sequencing. Plasmids were transiently transfected into HEK293 cells or THP-1 cells with lipofectamine 3000 reagents according to the manufacturer’s instructions.
Proximity ligation assay (PLA)
PLA was performed using the Duolink In Situ Red Kit purchased from Sigma-Aldrich (DUO92101) in accordance with the manufacturer’s instruction. Briefly, transfected cells were washed once with ice cold PBS, followed by fixation with 4% paraformaldehyde for 15 min at room temperature. Fixed cells were then washed three times with PBS and permeabilized with 0.5% Triton X-100 containing PBS for 10 min. Permeabilized cells were blocked with 5% normal goat serum for 1 h at room temperature. The cells were then incubated with primary antibodies diluted in 10% normal goat serum supplemented with 0.1% Tween at 4 °C overnight. Following the incubation, the cells were washed three times with PBS and then incubated with two PLA probes (Duolink In Situ PLA Probes Anti-rabbit PLUS and Anti-Mouse MINUS, Sigma-Aldrich) for 1 h at 37 °C. After probe incubation, the samples were incubated in ligation solution for 1 h at 37 °C. After ligation, cells were washed with Wash Buffer A and incubated in the amplification solution for 2 h at 37 °C. Cells were then serially washed twice in 1 × Wash Buffer B, 0.01 × Wash Buffer B once, and PBS once, followed by incubation with secondary antibodies for 1 h at room temperature. Finally, cells were washed three times with PBS and mounted in Duolink In Situ Mounting Medium supplemented with DAPI. Fluorescence images were obtained with a confocal microscope.
Co-immunoprecipitation(Co-IP)
Co-IP assays were performed according to standard protocols. For the Co-IP of Myc-ABHD5 and HA-TRIM59 proteins, anti-Myc and anti-HA agarose beads (20 μl) were used to pull down Myc-ABHD5 and HA-TRIM59, respectively. The protein-antibody complexes recovered on beads were subjected to western blotting using appropriate antibodies after separation by SDS-PAGE. The purified IgG was used as a negative control.
Ubiquitination assay
For ubiquitination assays, cells were transfected with vectors, including vectors expressing Myc-ABHD5, HA-TRIM59 and His-Ub, respectively, for 24 h. Cells were then treated with MG132 (10 μM) (Sigma-Aldrich) for 8 h, and the levels of Myc-ABHD5 ubiquitination was determined by IP with an anti-Myc antibody followed by western blot assays with an anti-His antibody (Cell Signaling Technology, Cat: #3936).
Protein half-life analysis
A cycloheximide (CHX) (Sigma-Aldrich) blocking analysis was performed to determine the half-life of ABHD5. Cells were incubated with CHX (50 μg/ml) for various times, and ABHD5 was detected by western blot analysis. The density of the immunoreactive bands corresponding to ABHD5 and β-actin were measured. The level of ABHD5 was quantified by normalization with β-actin, and the percentage of remaining ABHD5 was plotted.
Cell proliferation assay
LC cells proliferation was assessed using the EdU Assay Kit (RiboBio Inc., China) according to the manufacturer’s instructions.
Transwell invasion assays
The effect of conditioned medium of macrophages on the invasion of cancer cells was determined by using transwell 24-well plates (8 μm pore size, BD Biosciences). Cells were allowed to invade through the Matrigel (BD Biosciences) for 48 h. Migrated cells were fixed with 4% paraformaldehyde and stained with 1% crystal violet. For each chamber, three fields were randomly chosen and cells were counted.
Lactate secretion and lipid droplets content measurement
THP-1 macrophages at different treatments and regular culture medium was discarded and replaced with 2 ml fresh culture medium then culture for another 24 h, the lactate concentration was measured by lactate assay kit (Sigma-Aldrich, Cat: MAK064).
THP-1 macrophages (5 × 105 cells per well) at different treatments were rinsed in PBS once (1 min) and then fixed in 4% paraformaldehyde for 10 min. After rinsing with PBS for 3 times, 3 min each, cells were stained with Lipid (Oil Red O) Staining Kit (Sigma-Aldrich, Cat: MAK194). For lipid droplets content quantification, Oil Red O dye was extracted with DMSO and optical density (OD) was detected using a spectrophotometer at 496 nm.
Measurement of cytokine production
THP-1 macrophages or BMDMs were seeded in 24-well plates and cultured overnight. After priming with 1 μg/ml LPS for THP-1 macrophages or 100 ng/ml LPS for mouse BMDMs and stimulating with Nigericin (50 μM), ATP (5 mM), Alum (200 μg/ml), poly(dA:dT) (200 ng/ml) or flagellin (200 ng/ml), the supernatants were collected, and the concentrations of IL-1β were measured using ELISA kits (R&D Systems, Cat: #201-LB), according to the manufacturer’s instruction.
Multiplex immunohistochemistry
A LC tissue microarray containing 90 cases of LC and paired adjacent non-cancerous tissue was purchased from Shanghai Outdo Biotech (HLugAde180Sur01). For mIHC staining, Opal 4-color fluorescent IHC kit (Akoya Biosciences, Cat: #NEL810001KT) was used. First, the concentration and the application order of the three antibodies were optimized, and the spectral library was built based on the single-stained slides. The slides were first deparaffinized by xylene and ethanol and antigen retrieval was performed by microwave. After incubating with 3% H2O2 (freshly made) for 10 mins, the tissues were blocked in blocking buffer for another 10 mins at room temperature. Then the tissues were incubated by primary antibody, secondary-HRP (Cell Signaling Technology) and Opal working solution (Akoya Biosciences). The slides then were mounted with ProLong Gold Antifade Reagent with DAPI, and were scanned using confocal microscopy. Then several 20x fields (3–5 per sample) were analyzed at regions of interest (10–15 per field) identified along tumor and stromal zones. TRIM59 and IL-1β mean fluorescence intensities (MFIs) were measured, respectively. The results were confirmed by two experienced pathologists blinded to the clinicopathological parameters. Image J software was used for image quantification.
Isolation of peritoneal macrophages (PMs)
Each mouse was injected (i.p.) with 2 ml of 3% thioglycolate on day 1 and killed on day 3. After i.p. injection of 5 ml DMEM cell culture medium containing 10% FBS, as well as penicillin and streptomycin, the peritoneal cells were collected in cell culture dishes. Two hours later, the floating cells were removed by washing the cells with phosphate-buffered saline (PBS). The attached cells were considered to be PMs (purity: 90%) and were subjected to further experiments.
Isolation TAMs by magnetic-activated cell sorting (MACS) separation
Fresh lung tumor tissues were prepared as single cell suspensions, by minced in the DMEM with 0.1% collagenase I (1 h, 37 °C) and filtered using a 40 μm cell strainer (BD Falcon). Cells in suspensions were stained for 30 min at 4 °C with anti-mouse FITC-F4/80 antibody (eBiosience). After rigorous washing with 0.5% BSA and 2 mM EDTA in PBS, cells were incubated with an anti-FITC Microbeads (Miltenyi Biotec) for 20 min at 4 °C. F4/80+ cells were sorted by MACS separation according to the manufacturer’s instruction (Miltenyi Biotec), then F4/80+ cells (TAMs) and pass-through cells (mainly tumor cells) were prepared.
Animal experiments
The transgenic mice with macrophages overexpression of TRIM59 (TgTRIM59) were constructed (The cDNA of mouse TRIM59 was subcloned into a construct containing the human CD11b promoter to drive macrophage-specific gene expression, on a C57BL/6 J background, Model Animal Research Center, Nanjing University). We utilized 6 to 8-week-old C57BL/6 J WT and TgTRIM59 mice in this study. The mice were subcutaneously injected with lewis lung carcinoma (LLC) cells (5 × 106 cells per mouse). The tumor size of mice was measured. After 16 days, the mice were sacrificed and their tumors tissues were determined for histological examination. Tumor volume was determined using the formula: Volume = width × length × (width + length)/2.
In vivo metastasis, the C57BL/6 WT and transgenic mice were intravenously injected with LLC cells (5.0 × 106 cells per mouse) via the tail vein. The mice were sacrificed 14 days after tumor inoculation. All the suspicious lung metastasis sites were evaluated by histologic examination. All animal experiments were approved by the Institutional Animal Care and Use Committee and performed according to the institution’s guidelines and animal research principles.
Statistical analysis
All values are expressed as the mean ± SEM. Statistical analysis were performed with GraphPad Prism 7. Student’s t tests were used to evaluate continuous variables between subgroups. Values of P < 0.05 were considered to be statistically significant.
Discussion
Modification of the microenvironment allows tumor cells to overcome inhospitable extracellular conditions and progress towards metastasis. Recent studies have highlighted the key involvement of exosomes in the facilitation of tumor microenvironment [
28,
29]. Exosomes convey information to neighboring or remote cells by delivering proteins and RNAs thus affecting signaling pathways in various physiological and pathological conditions including cancer [
28]. Exosomes serve as communication vehicles between the tumor cells and their surroundings, favoring secretion of growth factors, cytokines, and angiogenic factors by stromal cells, fibroblasts, immune and inflammatory cells [
30]. In this study, we demonstrated that interaction between LC cells and macrophages by exosomal TRIM59 results in dysregulated NLRP3 inflammasome activity that drive macrophages-mediated tumor progression. First, TRIM59 can be transferred by exosomes from tumor cells to macrophages. Second, exosomal transfer of TRIM59 directly interacts with ABHD5 and increases ubiquitin- and proteasome-mediated ABHD5 degradation. Finally, ABHD5 degradation triggers NLRP3 signaling activation in macrophages, which facilitates secretion of IL-1β, resulting in increased proliferation and migration potential of LC cells.
Emerging clinical evidence shows that TRIM59 were frequently up-regulated in tumor tissues compared with noncancerous tissues from breast and gastric tumors, where increased levels were correlated with advanced clinical stages and reduced survival among cancer patients [
16,
31]. TRIM59 promotes tumor growth in hepatocellular carcinoma and regulates the cell cycle by degradation of protein phosphatase 1B [
18]. In addition, TRIM59 as an essential tumor-promoting factor that facilitates breast cancer growth and metastasis through modulating PDCD10-associated signaling pathways [
16]. It is now clear that cancer progression and metastasis depend on the bidirectional interactions between cancer cells and their environment, forming the tumor microenvironment. However, the pro-oncogenic role of TRIM59 in the tumor microenvironment have not yet been identified. In this study, exosomes secreted from lung cancer cells were isolated. We found that TRIM59 was detected in the exosomes of lung cancer cells, whereas the levels of TRIM59 in both lung cancer cells and exosomes were strongly decreased by transfection of shTRIM59. Moreover, these exosomes were found to enter into the macrophages in a dose and time-dependent manner. These results clearly demonstrated that the TRIM59 can be delivered into the macrophages by exosomes and implied that TRIM59 has functional roles in tumor microenvironment.
Macrophages represent a major component of the lympho-reticular infiltrates in solid tumors and play a crucial role in cancer progression [
32]. Notably, it has been well accepted that TAMs in cancer tissues bear complicated characteristics rather than simply M2 or M1, and, it is noteworthy that TAMs exhibit non classical M2/M1 phenotypes [
33]. In fact, a functional plasticity of TAMs has been proposed, wherein macrophages show an inflammatory phenotype in the early phase of tumor establishment, while displaying an immunosuppressive phenotype in the later phase of tumor progression [
32]. Previous study suggested that TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1 [
34]. Interestingly, in this study, we demonstrated that macrophages were educated by TRIM59 promote lung cancer cells progression. When viewed in combination, it is tempting to speculate that the heterogeneity and dynamic nature of tumor microenvironment across different cancers, as well as different stages of the same cancer, it is quite likely that the function of TRIM59 in TAMs might also vary across these conditions. Thus, the precise regulatory mechanisms of TRIM59 expression in the macrophages require further exploration in future studies.
Recently, multiple lines of evidence suggest that ABHD5 acts as a tumor suppressor because it plays a role in inflammation and lipid metabolism in cancer [
24]. Mechanistically, ABHD5 deficiency promotes colorectal tumor development by inducing glycolysis and epithelial-mesenchymal transition [
24]. ABHD5 is an intracellular lipolytic activator whose deficiency causes lipid overload in most cells including macrophages [
25,
35]. Metabolic adaptation is a key feature of macrophage plasticity and polarization and is instrumental to macrophage function in homeostasis, immunity, and inflammation [
26,
27,
36]. In this study, we demonstrated that the metabolic profile of THP-1 macrophages shifted in ABHD5 deficiency macrophages. Emerging data support the concept that metabolic regulators control macrophages activation [
26,
37]. Metabolic reprogramming in macrophages by certain stimulators is an important characteristic feature of TAMs that primarily affects their extrinsic regulatory functions in the tumor microenvironment [
27]. Recent reports have suggested that ABHD5 was highly expressed in TAMs in the tumor microenvironment. Moreover, macrophage ABHD5 enhanced the growth of colorectal cancer cells by suppressing spermidine synthesis [
38]. Interestingly, ABHD5 expression was heterogeneous in TAMs and that ABHD5
low macrophages (a macrophage subpopulation with decreased expression of ABHD5) potentially facilitate tumor cell migration and cancer metastasis [
23]. However, ABHD5 expression downregulated in metastasis-associated TAMs are not fully understood. Herein, our results showed that the ubiquitination of ABHD5 by an E3 ubiquitin ligase, TRIM59, promoted ABHD5 proteasomal degradation in macrophages. Thus, we conclude that lung cancer cells secrete exosomal TRIM59 that directly targets ABHD5, leading to ABHD5 deficiency in TAMs. Our results extend our knowledge regarding the regulation of TRIM59 and potential targets for the development of novel therapeutic approaches targeting cancer metastasis.
It is well established that, an intimate relationship among IL-1β, the NLRP3 inflammasome and the metabolism of lipids and carbohydrates in TAMs [
39]. IL-1β is a prominent proinflammatory cytokine, as it can efficiently cause the generation of other inflammatory mediators through signaling via IL-1 receptor, thus initiating a self-amplifying cytokine network [
40]. IL-1β is considered to be indicators of an increased risk of carcinoma and poor prognoses in multiple cancers [
41]. In the present study, we further revealed that the exacerbation of NLRP3 inflammasome activation by ABHD5 deficiency, provides a positive feedback loop to promote cancer progression by preferentially secrete the proinflammatory cytokine IL-1β. We have comprehensively linearized the TRIM59/ABHD5/NLRP3 signaling pathway and provided the concrete evidence showing such signaling events play essential roles in reprogramming macrophages and leading to the activation of oncogenic macrophages functions.
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