Ceritinib is a novel triple negative breast cancer therapeutic agent

MDA-MB-453 is a representative LAR TNBC cell line according to gene expression profiling [18]. MDA-MB-453 has high AR expression and is responsive to androgen stimulation or anti-androgen treatment [19]. Using MDA-MB-453 as a cell model, we performed a cytotoxicity assay and screened 133 FDA approved cancer specific drugs (Suppl Fig. 1A). Cells were treated with a final concentration of 10 μm of drugs and cell viability was determined by MTT assays (Suppl Fig. 1B). The detailed list of drugs that resulted in < 50% of total cell viability is shown in Suppl Fig. 1C. Interestingly, we found that ceritinib and crizotinib were among the most effective drugs against MDA-MB-453 cells (Suppl Fig. 1C). Ceritinib (LDK378) and crizotinib are second-generation inhibitors of anaplastic lymphoma kinase (ALK). They are FDA-approved drugs for the treatment of ALK⁺ metastatic non–small-cell lung cancer (NSCLC). Ceritinib is designed to target the highly conserved ATP binding site in the active ALK1. Due to the similarity of ATP binding sites in all tyrosine kinases, ceritinib also targets ROS1, IGF-1R, FAK, ACK1/TNK2 and many other tyrosine kinases besides ALK1 [20‐22]. The off-target effects of small molecule inhibitors are often an undesired phenomenon in the context of drug development as they are synthesized with the intent to generate selective and potent inhibitors. However, it can be beneficial in the search for novel TNBC treatments since ROS1, ACK1, IGF-1R and FAK are all potential therapeutic targets in TNBCs [23‐25]. Since ceritinib targets IGF-1R, ERK1/2, PI3K, FAK and ACK1/TNK2 [20], which are important players in androgen-independent AR signaling pathways, we primarily focused our studies on ceritinib.

We further explored the potential of ceritinib to induce cancer cell death in LAR TNBC cells. Ceritinib treatment killed MDA-MB-453 and another representative LAR cell line, MFM223, in a dose- and time-dependent manner (Suppl Fig. 2A B, C, D). The calculated IC₅₀ of ceritinib was 1.19 μM for MDA-MB-453 cells (Suppl Fig. 2B) and 1.24 μM for MFM223 cells (Suppl Fig. 2D). The standard administration for advanced NSCLC is 750 mg/day ceritinib (oral), which results in the mean of the maximal plasma concentration (C_max) at 1.43 ± 0.37 μM [22]. Therefore, IC₅₀ of ceritinib for MDA-MB-453 and MFM 223 is clinically achievable. Ceritinib treatment inhibited colony formation in MDA-MD-231, MDA-MB-453 and SUM185PE (Suppl Fig. 3A, B). Because TNBC is a highly heterogeneous cancer, we additionally examined the efficacy of ceritinib on four different human TNBC cell lines. Ceritinib efficiently killed another LAR cell line SUM185PE and three non-LAR TNBC cells MDA-MB-231, MDA-MB 157 and MDA-MB 468 in a dose- and time-dependent manner (Suppl Fig. 4 A, B, C, and D).

Ceritinib exhibits activity against ALK, ROS1, IGF-1R, FAK, ACK1/TNK2 and many other tyrosine kinases [20‐22]. To determine which RTKs targeted by ceritinib, we employed a RTK phosphorylation antibody array that contains several proteins [26]. We found that ACK1 is a major ceritinib target in MDA-MB-453 cells (Fig. 1 A and B). ACK1 is one of the major activated tyrosine kinases in aggressive TNBC cells according to a global phosphotyrosine proteasome study by John Hopkins University [27]. Another study also revealed ACK1 is a potential TNBC therapeutic target [24]. As a cytoplasmic kinase, ACK1 activates AR in prostate cancer [28]. However, it is not clear whether ACK1 and AR protein expression is associated in breast cancer. To explore the importance of ACK1 and AR in breast cancer, we analyzed their expression in a panel of 14 human breast cancer cell lines and 13 human breast cancer tissues by Western blot assay. We found 64% (9 of 14) and 57% (8 of 14) of cells expressed ACK1 and AR, respectively (Fig. 1 C). Similarly, 57 and 64% of human breast tumor tissues expressed ACK1 and AR, respectively (Fig. 1 D). The majority of breast cells and tissues tested concurrently expressed ACK1 and AR (Fig. 1 C and D) suggesting that there is a link between ACK1 and AR.in breast cancer.

Cell surface RTKs including IGF-IR, PDGFR, EGFR and HER3 are active in many TNBCs [29] (Fig. 1 C and D). RTKs can initiate androgen-independent AR signaling pathways, in which RTKs activate ACK1, which then forms a complex with AR and phosphorylates tyrosine residues in AR. Phosphorylated AR translocates to the nucleus with ACK1 and promotes transcription of oncogenes [28, 30]. To determine the importance of the RTK-ACK1-AR signaling axis as a possible mechanism for ceritinib-induced cytotoxicity in the AR⁺ TNBCs, we examined protein levels of AR, ACK1, HER2 and HER3 after ceritinib treatment in LAR cells. Ceritinib treatment downregulated AR, ACK1, HER2 and HER3 in MDA-MB-453 and MFM223 cells in a time-dependent manner (Fig. 2 A and B). For unknown reasons, Her3 is degraded. HER3 is a catalytically impaired pseudokinase. It is involved in the tumorigenesis in many kinds of cancers. HER3 could form powerful carcinogenic heterodimers with EGFR or HER2. Her2-Her3 heterodimers are potent signaling units. Even through MDA-MB 453 is considered a triple negative breast cancer, Her2 and HER3 are highly expressed in MDA-MB-453 [31]. Little is known about mechanisms of the Her3 downregulation from the plasma membrane, but there is evidence showing that Her3 is internalized and degraded in a clathrin-dependent manner [32]. The data suggested that ceritinib treatment inhibited RTK-ACK1-AR signaling pathways in LAR cells. In addition, activation of signaling pathways downstream of RTKs, including AKT-mTOR and MAPK cascades, are critical for TNBC tumorigenicity. Ceritinib treatment efficiently inhibited the phosphorylation of AKT, ERK1/2, S6, S6K1 and 4-EBP1, which are major players in AKT-mTOR and MAPK pathways (Fig. 2 C and D).

WNT deregulation is a major oncogenic driving force in TNBC. AR drives WNT and HER2 signaling pathways by transcriptional upregulation of WNT7B and HER3 [33]. We found that ceritinib treatment significantly suppressed the expression of proteins that are major players in the Wnt signaling pathway, including Axin1, LRP6 and β-catenin in multiple cell lines (Fig. 3 A and B). Cyclin D1 is a target gene of β-catenin. Ceritinib treatment suppressed the protein level of cyclin D1 (Fig. 3 A and B). Aberrant activation of Wnt signaling has been associated with the cancer stem cell (CSC) phenotype in breast cancer. AR maintains a CSC-population in TNBCs [34]. The existence of drug-resistant cancer stem cells (CSCs) is one of the main reasons why cancers are so difficult to treat effectively. Thus, we hypothesized that ceritinib treatment could overcome drug resistance through the presence of CSC populations by suppressing AR and Wnt signaling pathways. Indeed, we found that ceritinib treatment significantly decreased expression of the CSC marker ALDH1/2. In addition, multiple ABC transporters including ABCG2 enhance drug resistance in breast cancer. We found that ceritinib inhibited the expression of ABCG2 (Fig. 3 C and D). Together, these data indicate that ceritinib potentially inhibits the CSC population (Fig. 3 E) in LAR TNBC to overcome drug resistance in TNBC.

Enzalutamide blocks AR canonical signaling and significantly inhibits dihydrotestosterone (DHT)-driven growth of LAR cells [11]. To achieve a more effective treatment response, there is a strong clinical interest in developing new therapeutic strategies by combining AR antagonists with other targeted drugs. Our data show that enzalutamide inhibits cell growth of SUM185, MDA-MB-157, MDA-MB-231 and MDA-MB-468 (Suppl Fig. 5A, B, C, D). Current clinical trials examine several combinations of drugs including AR inhibitors plus PI3K-mTOR inhibitors and AR inhibitors plus CDK4/CDK6 inhibitors in advanced AR-positive TNBC tumors [14]. RTKs including IGF-IR, PDGFR, EGFR and HER3 are active in many TNBCs [14]. We found that ceritinib downregulated androgen-independent RTK-AR signaling (Fig. 2). However, ceritinib treatment did not inhibit RTK-AR signaling in the presence of DHT (Fig. 4 A), suggesting a cross-talk between the canonical AR signaling and androgen-independent AR signaling pathways. The activation of androgen-dependent AR signaling would compensate for the inhibition of androgen-independent AR signaling pathways. Therefore, we hypothesized that the combination of ceritinib and enzalutamide would provide enhanced effects on tumor growth inhibiting activity in AR⁺ TNBCs. To test the hypothesis, we treated MDA-MB-453 and SUM185PE LAR cells with ceritinib, enzalutamide, and the combination of ceritinib and enzalutamide compared to vehicle control. We found that the combination of ceritinib and enzalutamide has a robust inhibitory effect on cell growth of MDA-MB-453 and SUM185PE cells (Fig. 4 B and C). We then translated our in vitro findings to in vivo conditions and we established SUM159PT mouse xenograft models in female SCID mice (Jackson Laboratory) by subcutaneous injections as described previously [35]. Ceritinib or enzalutamide treatment alone showed only a modest inhibition of tumor growth. However, the combination of ceritinib and enzaluamide acted synergistically to suppress tumor progression with more robust inhibition (Fig. 4 D). We calculated the Bliss score for a block of ceritinib combination with enzalutamide by using a drug interaction reference model introduced by C. I. Bliss in 1939, we found that ceritinib and enzalutamide combination treatment synergistically suppressed the growth of AR⁺ TNBC cells MDA-MB 453 and SUM185PE. In addition, we also showed ceritinib and enzalutamide combination treatment synergistically suppressed AR⁺ TNBC SUM159PE growth (Suppl Fig. 15).

FAK is a non-receptor tyrosine kinase and is often overexpressed in prostate cancer. FAK can promote androgen-independent AR stimulation in prostate cancer. FAK is the downstream of ACK1 in breast cancer [36]. To understand the role of FAK and ACK, we treated MDA-MB-453 and SUM185PE cells with FAK specific inhibitor (PF-573228) and ACK1 specific inhibitor (AIM-100). We found that both FAK inhibitor and ACK1 inhibitor suppressed MDA-MB-453 and SUM185PE cell proliferation in a dose dependent manner, and the cells were more sensitive to FAK inhibitor than ACK1 inhibitor (Suppl Fig. 10 A and B). However, ceritinib in combination with FAK inhibitor but not ACK1 inhibitor suppressed MDA-MB-453 and SUM185PE cell proliferation in a significant synergistic manner compared to single agent treatment alone Suppl Fig. 11 and 12). Since we have seen a significant synergistic effect when we used ceritinib with FAK inhibitor (but not ACK1 inhibitor), we then checked for FAK, p-FAK, and FAK downstream targets, YB1, and p-YB1 in MDA-MB-453 and SUM185PE cells. Combination of Ceritinib with FAK inhibitor suppressed the p-FAK and p-YB1 suggesting an inhibitory mechanism when combining these drugs (Suppl Fig. 13 A and B).

Furthermore, our immunohistochemical analysis indicated that combination treatment had more robust inhibition of cell proliferation (Ki67), angiogenesis (CD31) and YB1 signaling (Suppl Fig. 7C). These data suggest the combination of enzalutamide and ceritinib may be used as a novel approach for treating AR⁺ TNBC patients (Fig. 4 E).

We have proposed a combination therapeutic strategy for AR⁺ TNBCs. However, nearly half of TNBC patients do not have AR and many of them only express very low level of AR. It is possible that AR⁻ or AR-low TNBC tumors can also benefit therapeutically with ceritinib treatment. Paclitaxel (PTX) is an FDA approved chemotherapeutic agent in TNBC [37]. However, a substantial population of TNBC patients develop resistance to PTX therapy [38]. Thus, new therapeutic approaches are needed to overcome PTX resistance in TNBC treatment. Increased FAK expression is associated with metastasis and poor prognosis in breast cancer [25]. Inhibition of FAK is one proposed mechanism to overcome YB-1 mediated PTX resistance in ovarian and lung cancers [39]. As shown before, we found that ceritinib in combination with FAK inhibitor but not ACK1 inhibitor suppressed MDA-MB-453 and SUM185PE cell proliferation in a significant synergistic fashion compared to single agent treatments (supplementary Figs. 12 and 13). To explore the importance of FAK and YB-1 in breast cancer, we first analyzed their expression in a panel of 14 human breast cancer cell lines by western blot (WB) assay. We found that all human breast cancer cell lines expressed FAK and YB-1 (Fig. 5 A). In addition, we observed breast cancer patient tumors also express FAK and YB-1 (Fig. 5 B).

Upon integrin-fibronectin interaction or RTK activation by EGF, FAK is activated by tyrosine phosphorylation on a critical residue at 397 (Y397) (Supp Fig. 6 A). Ceritinib has been shown to inhibit FAK activity in melanoma and lung cancers [20, 21]. Consistent with this, we found that ceritinib significantly inhibited the FAK-YB-1 axis, as evidenced by decreased phosphorylation levels of proteins downstream of FAK activation including pFAK Y397, pAKT S473, pYB-1 S102 (Fig. 5 C, D and F, Sup Fig. 6B and C). Furthermore, ceritinib treatment inhibited FAK-YB1 activity in MDA-MB-231 cells upon EGF stimulation. (Fig. 5 E and F).

The combination of FAK inhibition and paclitaxel has a strong effect in ovarian and lung cancers [21, 39]. Based on this, we hypothesized that inhibiting FAK by ceritinib will improve PTX-induced apoptosis in AR⁻ or AR^low TNBCs, and the combination of ceritinib and PTX will have an enhanced inhibitory effect on tumor cell growth. MDA-MB-231 and MDA-MB-468 cells are AR^low TNBC, which express high levels of FAK [40]. To test the hypothesis, we treated MDA-MB-231 and MDA-MB-468 cells with DMSO (control), ceritinib, PTX, and the combination of ceritinib and PTX, and found that the combination of ceritinib and PTX had a robust inhibitory effect on cell growth of MDA-MB 231 and MDA-MB 468 cells (Fig. 6 A and B). To further explore the therapeutic utility of the combinational therapy of ceritinib and PTX in vivo, we inoculated NSG mice with MDA-MB-231 cells. Strategy is shown in Fig. 6 C. Four treatment groups were included, (1) untreated control, (2) ceritinib (25 mg/kg bodyweight) alone, (3) PTX alone (10 mg/kg of bodyweight), and (4) ceritinib (25 mg/kg bodyweight) + PTX (10 mg/kg of bodyweight). Overall, ceritinib or PTX alone treatment decreased tumor progression. The combination of ceritinib and PTX showed robust inhibitory effect on the tumor growth and lung metastasis, compared to single drug alone (Fig. 6 D, E and F). These findings are partially due to a reduction in cell proliferation as demonstrated by Ki-67 staining of tumors (Supplementary Fig. 7A, B). To make our results more clinically relevant, we tested our hypothesis using the PDX model TU-BcX-4EA-LNb. We implanted intact tumor pieces coated in Matrigel™ in NSG mice and treated the tumors after they reached a designated volume in groups outlined in the previous xenograft experiment. We demonstrated TU-BcX-4EA-LNb to exhibit high expression of both AR and ACK1 (Supplementary Fig. 8). Baseline AR and ACK1 expression were also tested in other TNBC PDX models (Supplementary Fig. 8A). Two additional TNBC PDX models exhibited moderate expression of AR (TU-BcX-4 M4, TU-BcX-4IC) but to a dramatically lesser extent than TU-BcX-4EALNb. ACK1 expression was more variably expressed in the TNBC PDX tumors tested (Supplementary Fig. 8B).

Next we examined the effect using the TNBC PDX model that expressed AR and ACK1. Although ceritinib and PTX alone inhibited tumor growth, combination treatment with both drugs together much stronger inhibition of tumor growth (Fig. 7 A, B). Importantly, there was no change in body weight suggesting minimal toxic effects of the combination treatment (Fig. 7 C). Furthermore, ex vivo ceritinib and PTX combination treatment significantly increased apoptosis in PDX tumors as exhibited by increasing cleaved caspase 3 and reducing MCL1 (Fig. 7 D). To show the therapeutic outcomes and confirm the cellular mechanistic data, we isolated the fresh 4QATb2 PDX tumor and cut it to 3 × 3 mm³ small pieces, and treatment them with DMSO (control), ceritinib (Ceri), paclitaxel (PTX) and Ceri + PTX for 48 h, the treated samples were examined by western blot assays. We found that ceritinib and enzalutamide combination treatment significant suppressed the p-FAK and pYB-1 than single treatment (Fig. 7 E). These data strongly suggest that the combination of ceritinib and PTX is more effective than single drug alone in AR⁻ and AR ^low TNBC cancers.

In our study, we found that ceritinib inhibited the RTK-ACK1/FAK-AR axis in LAR TNBC cells. Consistent with this, data collected from public databases indicate that ACK (TNK2 gene) positively correlates with AR, Her3 (ERBB3), WNT signaling proteins such as Axin 1, β-catenin and cyclin D1 (Supplementary Fig. 9).

All the mice were housed in a pathogen-free animal room under standard conditions with free access to water and food (standard chow diet and water ad libitum). All of the procedures were approved by the Institutional Animal Care and Use Committee of the LSU Health Science Center at New Orleans.

MDA-MB-231, MCF7, T47D, SKBR3, BT474, BT-20, MDA-MB-468, Hs578t and MDA-MB-453 and MDA-MB-157 cells purchased from the American Type Culture Collection (Manassas, VA). MFM 223, SUM159PE and SUM185PE cells were kindly provided by Dr. Jennifer A. Pietenpol (Vanderbilt-Ingram Cancer Center). ACK1, AR, EGFR, Her2, Her3, Mcl-1, Bcl-2, Puma, YB-1, FAK, AKT, Anti-phospho-AKT (Ser473), anti-phospho-S6K1 (S79), Anti-phospho-4-EBP1, Anti-phospho-LRP6, Anti-phospho-mTOR, Anti-phospho-YB-1, Anti-phospho-FAK (Y397), anti- ribosomal protein S6, anti-ribosomal protein S6, BCL-xL and anti-cleaved Casp3 antibodies were obtained from Cell Signaling Technology (Beverly, MA). Anti-phospho-ribosomal protein S6 (S235/236), mouse anti-ERK antibody and Anti-phospho-ERK were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Vinculin was purchased from Sigma. Secondary anti-mouse IgG with horseradish peroxidase was from Calbiochem. Secondary anti-rabbit IgG with horseradish peroxidase was from GE Healthcare. Ceritinib and Paclitaxel were purchased from APExBIO. Bicalutamide (97%) purchased from ACROS Organics. The 133 FDA-approved drugs were kindly provided from NCI/DTP Approved Oncology Drugs Plated Set (AODVIII).

To determine which receptor tyrosine kinases (RTKs) are targeted by ceritinib, the Human RTK Phosphorylation Antibody Array C1 Kit (RayBiotech, Cat. No. AAH-PRTK-1-2) was used. MDA-MB-453 cells were grown in DMEM medium containing 10% fetal bovine serum (FBS) and penicillin/streptomycin. When cells reached 70 ~ 80% confluence, media were changed to DMEM medium supplemented with DMSO, or 10 μM ceritinib. After 4 h incubation, cell lysates were prepared using lysis buffer containing protease and phosphatase inhibitors. After blocking for 1 hour at room temperature, the array membranes incubated with 700 μg of protein lysate overnight at 4 °C. Next, the arrays washed and incubated with biotin-conjugated phospho-tyrosine detection antibody overnight at 4 °C. Finally, the arrays incubated with a horseradish peroxidase-conjugated streptavidin (1:1000) at room temperature for 2 h. The phosphorylated RTKs detected and captured by ChemiDoc™ XRS+ Imaging Systems (Bio-Rad). The densitometric values of phospho-RTKs determined by an image lab software (Bio-Rad). The relative intensities of the duplicated spots were normalized to positive control spots. Values represent the mean of duplicate spots for each protein after normalization.

Tumor cells were seeded onto 6-well plates at a density of 300,000 cells per well. After culturing in complete DMEM medium for 16 hours, media was replaced with fresh DMEM containing 10% FBS and 10 μM ceritinib for 4 hours. In control conditions, media was replaced with fresh DMEM containing 10% FBS. Cells at indicated time points were lysed in radioimmunoprecipitation (RIPA) lysis buffer with protease inhibitor cocktail (Roche) and phosphatase inhibitors. Protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo, Waltham, MA). Samples were boiled in SDS sample buffer for 10 minutes and stored at − 80 °C until analysis.

TNBC cells were seeded (3000–5000 cells per well) in 96-well plates. Growth medium was replaced with either fresh medium (DMSO as a control) or medium containing the drugs after overnight growth. Cell viability was determined in quadruplicate using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, a colorimetric assay according to the conversion of tetrazolium salts to blue formazan products by active cells, in 96-well plates at the indicated time points. The replicates normalized to the control wells. To further confirm the results of MTT assay, the experiments were repeated using Cell Counting Kit 8 (WST-8 / CCK8) (Abcam, ab228554). The data analysis performed using Prism software (GraphPad Software). Data represent the mean ± SEM. Student’s t test was used to analyze the data, and a p-value of < 0.05 was considered statistically significant. * P < 0.05; ** P < 0.01, *** P < 0.0001.

Tissue sections were deparaffinized with xylene and re-hydrated through descending grades of alcohol up to water. Then, non-enzymatic antigen retrieval in Citrate buffer, pH 6.0 for 30 minutes at 95 °C, and endogenous peroxidase quenching with H2O2 in Methanol for 30 minutes were done. Sections were washed three times in PBS at interval of 10 minutes before blocking with 5% normal goat serum in 0.1% PBS/BSA. Tissues were incubated with Anti-Ki67 antibody (1:100 dilution; Cat# ab833, Abcam) overnight. After PBS washing, sections were incubated with a biotinylated anti-rabbit secondary IgG for 60 minutes, incubated with avidin-biotin-peroxidase (ABC) complexes, and developed with diaminobenzidine (Sigma).

MDA-MB 231 (1 × 10⁶ cells) with 50% Matrigel™ were injected into Number 4 mammary gland of 4-5 weeks old NSG (Jackson Laboratory). Tumors were measured by a caliper. When tumors reached a size of ∼50 mm³ the mice were randomly distributed into four groups (seven mice in each group): (1) untreated control, (2) ceritinib (25 mg/kg bodyweight) alone, (3) paclitaxel (PTX) alone (10 mg/kg of bodyweight), and (4) ceritinib (25 mg/kg of bodyweight) + PTX (10 mg/kg of bodyweight). Ceritinib was dissolved in sesame oil and administrated by oral gavage once daily for 2 weeks. PTX (10 mg/Kg) was injected intra-peritoneally twice a week. Tumor volume measured twice a week after the initial injection, and the volumes were calculated using the formula (π x length x width1 x width2 /6). Mice were euthanized when they became moribund, or when they lost 20% weight. All organs examined for the presence of tumors and metastases at autopsy. Metastatic tumors were defined as nodules identified at secondary sites with a diameter ≥ 0.2 mm. To examine whether lungs infiltrated by metastases, lungs were fixed in formalin and embedded in paraffin. Sections stained with hematoxylin & eosin (H&E) staining. Data grouped and plotted using GraphPad Prism 8.

SUM159PE (5 × 10⁶ cells) with 50% Matrigel™ were injected into Number 4 mammary glands of 4-5 week old SCID mice (Jackson Laboratory). Tumors were measured by a caliper. When tumors reached ∼100 mm³ the mice were randomly distributed into four groups that included seven mice in each group: untreated control, ceritinib (50 mg/kg bodyweight) alone, enzalutamide alone (25 mg/kg of bodyweight), and ceritinib (50 mg/kg bodyweight) + enzalutamide (25 mg/kg of bodyweight). Drugs were administrated by oral garage once daily for 2 weeks. Tumor volume was measured twice a week after the initial injection. All the mouse experiments were performed in accordance with procedures and guidelines approved by Institutional Animal Care and Use Committee of the LSU Health Science Center at New Orleans.

The AR-positive triple negative patient-derived tumor, designated as TU-4EA-LNb, was derived from a metastatic lymph node after mastectomy surgery with lymph node dissection from an African-American patient. TU-4EA-LNb was established in 4–6 week old SCID/Beige (CB17.Cg-PrkdcscidLyst bg/Crl) mice provided from Jackson Laboratory, a mouse type chosen to optimize tumor take. Tumor volume was measured using a digital caliper. Intact tumor pieces (3 × 3 mm²) were coated with Matrigel™ (BD Biosciences) and implanted unilaterally in the mammary fat pads of SCID/Beige mice. When tumors reached ∼100 mm³ the mice were randomly distributed into four groups: (1) untreated control, (2) ceritinib (50 mg/kg bodyweight) alone, (3) enzalutamide alone (25 mg/kg of bodyweight), and (4) ceritinib (50 mg/kg bodyweight) + enzalutamide (25 mg/kg of bodyweight). Drugs were administrated by oral gavage once daily for 2 weeks. Tumor volume was measured twice a week after the initial injection. For ex vivo analysis, TU-4EA-LNb tumor pieces were plated in cell culture dishes and covered in DMEM containing 10% FBS and drug treatments or vehicle controls. After the designated treatment time explants were collected, mechanically disrupted using scissors, and enzymatically digested using Trizol. Tumor pieces of additional PDX models established by the Burow lab were utilized to evaluate baseline expression of AR and ACK1: TU-BcX-2 K1 (T8, T11), TU-BcX-4 M4 (Tb2, Tb3), TU-BcX-4QX (Tb1, Tb2), TU-BcX-4IC (T1, T3) and TU-BcX-4EA-LNb (passage 4). The number following ‘T’ in the nomenclature of the PDX models after ‘TU-BcX-‘denotes the number of times the tumor had been serially transplanted in mice before the tumor was removed for analysis. All the mouse experiments performed in accordance with procedures and guidelines approved by Institutional Animal Care and Use Committee of the LSU Health Science Center at New Orleans. PDX tissues were procured through the Louisiana Cancer Research Consortium Biospecimen Core and processed following NIH regulations and institutional guidelines of Tulane University with IRB exemption status. All animal procedures were conducted in compliance with State and Federal laws, standards of the U.S. Department of Health and Human Services, and guidelines established by the Tulane University Animal Care and Use Committee. The facilities and laboratory animals programs of Tulane University are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care.

Fresh isolated tumor samples were snap frozen in liquid nitrogen. The extracts were prepared by grinding tissue into a fine powder in liquid nitrogen and subsequently dissolved in modified RIPA buffer supplemented with protease and phosphatase inhibitors. The lysates cleared by centrifuge at 13,000 rpm for 15 min and the supernatant was collected as extracted protein. Protein concentrations were determined using BCA protein assay. Equal amount of protein lysates separated by SDS-PAGE and then transferred to a PVDF membrane and detected with various antibodies.