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Erschienen in: Archives of Virology 9/2018

25.05.2018 | Brief Report

Characterization and application of monoclonal antibodies against channel catfish virus (CCV)

verfasst von: Hongli Jing, Min Zhang, Shaoqiang Wu, Xiangmei Lin, Longying Gao, Na Wang

Erschienen in: Archives of Virology | Ausgabe 9/2018

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Abstract

Three monoclonal antibodies (MAbs) (27E4, 17H2, 8B6) against channel catfish virus (CCV) were developed by immunizing Balb/C mice. Using indirect ELISA, these MAbs reacted only with CCV and not with three other fish viruses or nine fish cell lines. During western blotting analysis, MAb 27E4 recognized 170 kDa and 47 kDa proteins, while MAb 17H2 and MAb 8B6 recognized 47 kDa and 56 kDa proteins, respectively. Furthermore, a sandwich ELISA was developed for detection of CCV. The detection limit of the test was 105 TCID50/mL.
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Metadaten
Titel
Characterization and application of monoclonal antibodies against channel catfish virus (CCV)
verfasst von
Hongli Jing
Min Zhang
Shaoqiang Wu
Xiangmei Lin
Longying Gao
Na Wang
Publikationsdatum
25.05.2018
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 9/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3884-9

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