Introduction
Materials and Methods
Animals and Treatment
Drugs
Behavioral Study
Locomotor Activity
Biochemical Studies
Ex Vivo Experiments
Dopamine Metabolism and l-DOPA Metabolism
In Vivo Microdialysis Study and Analytical Procedure
Calculations and Statistics
Results
Behavioral Study
The Influence of Acute Salsolinol Administration on l-DOPA-Induced Hyperactivity in Rats
The Influence of Chronic Salsolinol Administration on l-DOPA-Induced Hyperactivity in Rats
Biochemical Studies
Ex Vivo Experiments
The Effect of Acute Salsolinol Administration on the l-DOPA-Induced Changes in Dopamine Metabolism in Rat Brain Structures
Treatment | DA (ng/g tissue) | DOPAC (ng/g tissue) | HVA (ng/g tissue) | [HVA]/[DA] × 100 | |
---|---|---|---|---|---|
Acute | Acute | ||||
Substantia nigra | |||||
Saline | Saline | 889 ± 51 | 212 ± 34 | 106 ± 15 | 12 ± 2 |
Saline |
l-DOPA 100 | 3985 ± 687** | 12,888 ± 2632** | 6208 ± 1000** | 187 ± 39** |
Salsolinol 100 | Saline | 773 ± 41 | 185 ± 14 | 102 ± 7 | 13 ± 0.8 |
Salsolinol 100 |
l-DOPA 100 | 4146 ± 724** | 13,981 ± 1066** | 5841 ± 403** | 188 ± 57** |
Effect of salsolinol
|
F
(1/27)
= 0.001
N.S.
|
F
(1/27)
= 0.05
N.S.
|
F
(1/27)
= 0.04
N.S.
|
F
(1/27)
= 0.001
N.S.
| |
Effect of
l-DOPA
|
F
(1/27)
= 21.85
P < 0.01
|
F
(1/27)
= 29.4
P < 0.01
|
F
(1/27)
= 40.8
P < 0.01
|
F
(1/27)
= 16.9
P < 0.01
| |
Interaction of salsolinol + l-DOPA
|
F
(1/27)
= 0.04
N.S.
|
F
(1/27)
= 0.05
N.S.
|
F
(1/27)
= 0.04
N.S.
|
F
(1/27)
= 0.001
N.S.
| |
Striatum | |||||
Saline | Saline | 9043 ± 430 | 1238 ± 25 | 675 ± 46 | 7.4 ± 0.3 |
Saline |
l-DOPA 100 | 17,802 ± 1967* | 19,266 ± 3690** | 10,057 ± 1587** | 57 ± 7.9** |
Salsolinol 100 | Saline | 9457 ± 411 | 1573 ± 109 | 1017 ± 83 | 11 ± 0.5 |
Salsolinol 100 |
l-DOPA 100 | 19,351 ± 3151** | 22,534 ± 2433** | 10,323 ± 503** | 62 ± 12** |
Effect of salsolinol
|
F
(1/27)
= 0.2
N.S.
|
F
(1/27)
= 0.3
N.S.
|
F
(1/27)
= 0.4
N.S.
|
F
(1/27)
= 0.2
N.S.
| |
Effect of
l-DOPA
|
F
(1/27)
= 17.8
P < 0.01
|
F
(1/27)
= 30.9
P < 0.01
|
F
(1/27)
= 40.8
P < 0.01
|
F
(1/27)
= 29
P < 0.01
| |
Interaction of Salsolinol + l-DOPA
|
F
(1/27)
= 0.07
N.S.
|
F
(1/27)
= 0.2
N.S.
|
F
(1/27)
= 0.001
N.S.
|
F
(1/27)
= 0.01
N.S.
|
Striatum
The Effect of Chronic Administration of Salsolinol on the l-DOPA-Induced Changes in Dopamine Metabolism in Rat Brain Structures
Treatment | DA (ng/g tissue) | DOPAC (ng/g tissue) | HVA (ng/g tissue) | [HVA]/[DA] × 100 | |
---|---|---|---|---|---|
Chronic | Acute | ||||
Substantia nigra | |||||
Saline | Saline | 844 ± 61 | 215 ± 18 | 152 ± 11 | 18 ± 2 |
Saline |
l-DOPA 100 | 4775 ± 898** | 19,039 ± 3135** | 9133 ± 622** | 249 ± 60** |
Salsolinol 100 | Saline | 913 ± 59 | 198 ± 14 | 146 ± 14 | 16 ± 1 |
Salsolinol 100 |
l-DOPA 100 | 2996 ± 623*+
| 5372 ± 4089**+
| 9256 ± 1008** | 337 ± 87** |
Effect of salsolinol
|
F
(1/24)
= 2.4
N.S.
|
F
(1/24)
= 0.5
N.S.
|
F
(1/24)
= 0.01
N.S.
|
F
(1/24)
= 0.6
N.S.
| |
Effect of
l-DOPA
|
F
(1/24)
= 30.1
P < 0.01
|
F
(1/24)
= 43.5
P < 0.01
|
F
(1/24)
= 233
P < 0.01
|
F
(1/24)
= 26.9
P < 0.01
| |
Interaction of salsolinol + l-DOPA
|
F
(1/24)
= 2.8
N.S.
|
F
(1/24)
= 0.5
N.S.
|
F
(1/24)
= 0.01
N.S.
|
F
(1/24)
= 0.7
N.S.
| |
Striatum | |||||
Saline | Saline | 10,439 ± 308 | 1467 ± 49 | 1149 ± 31 | 11 ± 0.5 |
Saline |
l-DOPA 100 | 21,031 ± 2530** | 28,780 ± 3700** | 14,858 ± 719** | 77 ± 10** |
Salsolinol 100 | Saline | 11,415 ± 394 | 1789 ± 124 | 1377 ± 112 | 12 ± 0.8 |
Salsolinol 100 |
l-DOPA 100 | 16,145 ± 1655*+
| 24,479 ± 5854** | 16,492 ± 1180** | 106 ± 10**++
|
Effect of salsolinol
|
F
(1/24)
= 1.6
N.S.
|
F
(1/24)
= 0.3
N.S.
|
F
(1/24)
= 1.8
N.S.
|
F
(1/24)
= 4.5
P < 0.05
| |
Effect of
l-DOPA
|
F
(1/24)
= 25
P < 0.01
|
F
(1/24)
= 52.1
P < 0.01
|
F
(1/24)
= 432
P < 0.01
|
F
(1/24)
= 128
P < 0.01
| |
Interaction of salsolinol + l-DOPA
|
F
(1/24)
= 3.6
N.S.
|
F
(1/24)
= 0.4
N.S.
|
F
(1/24)
= 1.0
N.S.
|
F
(1/24)
= 3.9
N.S
|
Striatum
The Impact of Acute and Chronic Administration of Salsolinol on l-DOPA Metabolism in the Rat Striatum
Treatment | 3-MDOPA (ng/g tissue) |
---|---|
Saline | 1.31 ± 0.11 |
Salsolinol 100 acute | 1.46 ± 0.1 |
Salsolinol 100 chronic | 1.39 ± 0.11 |
l-DOPA 100 | 17,429 ± 2874** |
Salsolinol 100 acute + l-DOPA 100 | 16,046 ± 1770** |
Salsolinol 100 chronic + l-DOPA 100 | 15,102 ± 3278** |
Effect of treatment |
F
(5/40) = 15.22
P < 0.01 |