Background
Methods
Subjects
Blood sample collection and storage
PCSK9, CETP activity and biochemical analysis
SNP selection and genotyping
Statistical analysis
Results
Participant characteristics and biochemical parameters
Variable | N = 450 |
---|---|
Age (years) | 60 (50–66) |
Gender (% men) | 51.3 |
BMI (Kg/m2) | 30.0 (26.9–35.0) |
SBP(mmHg) | 135 (125–149) |
DBP (mmHg) | 80 (72–86) |
AD (%) | 32.1 |
MetS (%) | 78.1 |
Obesity (%) | 50.6 |
T2DM (%) | 72.0 |
Cholesterol (mmol/L) | 5.20 (4.54–6.19) |
Triglycerides (mmol/L) | 1.54 (0.99–2.45) |
LDL-C (mmol/L) | 3.27 (2.55–4.07) |
HDL-C (mmol/L) | 1.40 (1.21–1.62) |
Glucose (mg/dL) | 128.5 (101.3–164.9) |
Apo B100 (mg/dL) | 101.0 (84.0–120.0) |
Apo A1 (mg/dL) | 137.9 ± 14.9 |
CETP activity (nmol/L per h) | 11.6 (10.2–13.3) |
LCAT activity (470/390 nm) | 2.14 (2.07–2.24) |
PCSK9 (ng/mL) | 319.9 (254.4–403.9) |
PCSK9 rs11591147 GG | 94.8 |
Genotypea, (%) GT | 5.2 |
Lp particle number | |
VLDL (nmol/L) | 49.7 (28.5–97.8) |
Large VLDL (nmol/L) | 1.83 (1.01–2.99) |
Medium VLDL (nmol/L) | 7.87 (4.52–13.6) |
Small VLDL (nmol/L) | 39.9 (22.7–82.2) |
LDL (nmol/L) | 892.3 (681.8–1175.8) |
Large LDL (nmol/L) | 113.4 (87.0–149.4) |
Medium LDL (nmol/L) | 319.4 (234.3–417.2) |
Small LDL (nmol/L) | 463.5 (337.9–618.5) |
HDL (µL/L) | 25.3 (21.6–29.4) |
Large HDL (µL/L) | 0.15 (0.11–0.19) |
Medium HDL (µL/L) | 6.94 (5.08–9.21) |
Small HDL (µL/L) | 17.9 (15.5–20.9) |
Lp size (diameter, nm) | |
VLDL | 42.6 ± 0.57 |
LDL | 21.0 (20.9–21.1) |
HDL | 8.19 (8.12–8.24) |
Associations between CETP activity and PCSK9 levels
CETP | PCSK9 | |||
---|---|---|---|---|
r |
P
| r |
P
| |
CETP | 1.000 | – | 0.256a
| <0.0001 |
PCSK9 | 0.256a
| <0.0001 | 1.000 | – |
Age | −0.088 | 0.063 | 0.045 | 0.346 |
BMI | 0.020 | 0.679 | 0.052 | 0.268 |
SBP | 0.208 | <0.0001 | 0.123 | 0.022 |
DBP | 0.129 | 0.016 | 0.085 | 0.112 |
Cholesterol | 0.585 | <0.0001 | 0.194 | <0.0001 |
Triglycerides | 0.491 | <0.0001 | 0.210 | <0.0001 |
LDL-C | 0.412 | <0.0001 | 0.179 | <0.0001 |
HDL-C | 0.134 | 0.004 | 0.109 | 0.021 |
ApoB100 | 0.494 | <0.0001 | 0.194 | <0.0001 |
ApoA1 | −0.002 | 0.970 | 0.135 | 0.004 |
Glucose | 0.186 | <0.0001 | 0.215 | <0.0001 |
LCAT | −0.251 | <0.0001 | −0.072 | 0.127 |
Lp particles number | ||||
Total VLDL | 0.419 | <0.0001 | 0.176 | <0.0001 |
Large VLDL | 0.411 | <0.0001 | 0.160 | 0.001 |
Medium VLDL | 0.413 | <0.0001 | 0.172 | <0.0001 |
Small VLDL | 0.420 | <0.000 | 0.177 | <0.0001 |
Total LDL | 0.300 | <0.0001 | 0.134 | 0.004 |
Large LDL | 0.246 | <0.0001 | 0.093 | 0.049 |
Medium LDL | 0.234 | <0.0001 | 0.111 | 0.019 |
Small LDL | 0.332 | <0.0001 | 0.152 | 0.001 |
Total HDL | 0.098 | 0.038 | 0.024 | 0.614 |
Large HDL | 0.109 | 0.021 | 0.043 | 0.359 |
Medium HDL | −0.193 | <0.0001 | −0.044 | 0.356 |
Small HDL | 0.241 | <0.0001 | 0.071 | 0.136 |
Lp size | ||||
VLDL | −0.276 | <0.0001 | −0.114 | 0.015 |
LDL | −0.200 | <0.0001 | −0.107 | 0.023 |
HDL | −0.362 | <0.0001 | −0.104 | 0.027 |
Adjusted for | CETP | ||
---|---|---|---|
Beta |
P
|
R
2
| |
PCSK9 | |||
Model 1: crude (no adjustment) | 0.253 | <0.0001 | 0.064 |
Model 2: age, gender and BMI | 0.268 | <0.0001 | 0.085 |
Model 3: model 2 + LDL-C, TG, and Glu | 0.107 | 0.001 | 0.582 |
Model 4: model 3 + SBP and LCAT | 0.107 | 0.004 | 0.624 |
Model 5: model 4 + MetS | 0.110 | 0.003 | 0.624 |