Introduction
Materials and methods
Selection of samples
RNA isolation and amplification
Immunohistochemistry
Microarray hybridisation
Processing of microarray data
Unsupervised hierarchical clustering
Supervised classification
Supplementary information
Results
Tumour characteristics
In situ samples | Invasive samples | ||
---|---|---|---|
Differentiation | Number (percentage) | Histological grade | Number (percentage) |
Well | 6 (15%) | 1 | 5 (12.5%) |
Intermediately | 18 (45%) | 2 | 11 (27.5%) |
Poorly | 14 (35%) | 3 | 24 (60%) |
Good/poor component | 1 (2.5%) | ||
LCIS | 1 (2.5%) | ||
IHC | IHC | ||
ER-positive | 28 (70%)a
| ER-positive | 22 (55%)c
|
PR-positive | 24 (60%)a
| PR-positive | 19 (47.5%)d
|
Her2/neu-positive (3+) | 12 (30%)b
| Her2/neu-positive (3+) | 4 (10%)d
|
p53-positive | 11 (27.5%)b
| p53-positive | 9 (22.5%)d
|
Tumour detection | |||
Palpation | 17 (42.5%) | ||
Microcalcifications | 18 (45%) | ||
Others | 5 (12.5%) | ||
Tumour diameter (mm) | |||
Range | 10 to 80 | ||
Median | 45 | ||
Average | 42.8 | ||
Treatment | |||
Mastectomy | 24 (60%) | ||
Breast conserving treatment | 6 (15%) | ||
Local excision followed by mastectomy | 10 (25%) |
Molecular subtypes of breast cancer
Unsupervised hierarchical clustering
Unsupervised hierarchical clustering of in situand invasive samples
Unsupervised hierarchical clustering of DCIS
Supervised classification
Supervised classification of in situversus invasive carcinomas
Rank | NKI ID | Symbol | Annotation | Accession no. |
---|---|---|---|---|
1 | 116810 |
ADM
| Adrenomedullin | AA446120 |
2 | 123346 | EST | H17315 | |
3 | 117289 |
MMP11
| Matrix metalloproteinase 11 (stromelysin 3) | AA045500 |
4 | 121066 |
DAPK3
| Death-associated protein kinase 3 | AA973730 |
5 | 123776 |
PIAS4
| Protein inhibitor of activated STAT protein | H30547 |
6 | 101837 |
DHX34
| KIAA0134 gene product | AA477623 |
7 | 102847 |
YIF1
| Putative transmembrane protein; homolog of yeast Golgi membrane protein Yif1p (Yip1p-interacting factor) | H79351 |
8 | 117345 |
ACTN1
| Actinin, alpha 1 | AA669042 |
9 | 127755 |
TGFB2
| Transforming growth factor, beta 2 | W47556 |
10 | 108960 |
GABRD
| Gamma-aminobutyric acid (GABA) A receptor, delta | H41122 |
11 | 108348 |
MFAP2
| Microfibrillar-associated protein 2 | N67487 |
12 | 129658 |
MGC13045
| DnaJ (Hsp40) homolog, subfamily C, member 4 | AA996059 |
13 | 105479 |
BAT3
| HLA-B-associated transcript-3 | AA434416 |
14 | 120649 |
KCTD5
| Hypothetical protein | AA521027 |
15 | 110728 |
FBXL15
| F-box and leucine-rich repeat protein 15 | T61547 |
16 | 120934 |
EIF4G1
| Eukaryotic translation initiation factor 4 gamma, 1 | R37276 |
17 | 118584 |
C9orf115
| ESTs, weakly similar to B36298 proline-rich protein PRB3S [Homo sapiens] | AA479713 |
18 | 105533 |
ARF1
| ADP-ribosylation factor 1 | W45572 |
19 | 131909 |
TUBB2
| Tubulin, beta polypeptide | AI672565 |
20 | 131540 |
PRPF31
| DKFZP566J153 protein | AI253017 |
21 | 110281 |
HSPA1L
| Heat shock 70-kD protein-like 1 | H17513 |
22 | 107215 |
KCTD5
| Hypothetical protein | AA429470 |
23 | 121937 |
FLJ10374
| Hypothetical protein FLJ10374 | AA676962 |
24 | 100368 |
GNB2
| Guanine nucleotide binding protein (G protein), beta polypeptide 2 | N68166 |
25 | 105453 |
PSAP
| Prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) | N72215 |
26 | 115391 |
LMCD1
| LIM and cysteine-rich domains 1 | AA452125 |
27 | 128198 |
MMP11
| Matrix metalloproteinase 11 (stromelysin 3) | AA954935 |
28 | 123688 |
COL1A1
| Collagen, type I, alpha 1 | R48844 |
29 | 127890 |
PTMS
| Parathymosin | AA458981 |
30 | 102044 |
DRAP1
| DR1-associated protein 1 (negative cofactor 2 alpha) | AA406285 |
31 | 101067 |
MAP7
| Microtubule-associated protein 7 | R77252 | R77251 |
32 | 129438 |
IQGAP1
| IQ motif containing GTPase activating protein 1 | AA478633 |
33 | 125700 |
APC2
| Adenomatous polyposis coli like | AA976241 |
34 | 127881 |
NFIC
| Nuclear factor I/C (CCAAT-binding transcription factor) | T59427 |
35 | 109065 |
SYT5
| Synaptotagmin V | H39018 |
Supervised classification for poorly differentiated DCIS versus grade 3 invasive carcinoma
Rank | NKI ID | Symbol | Annotation | Accession no. |
---|---|---|---|---|
1 | 123776 |
PIAS4
| Protein inhibitor of activated STAT protein | H30547 |
2 | 129658 |
MGC13045
| DnaJ (Hsp40) homolog, subfamily C, member 4 | AA996059 |
3 | 121937 |
FLJ10374
| Hypothetical protein FLJ10374 | AA676962 |
4 | 102847 |
YIF1
| Putative transmembrane protein; homolog of yeast Golgi membrane protein Yif1p (Yip1p-interacting factor) | H79351 |
5 | 127755 |
TGFB2
| Transforming growth factor, beta 2 | W47556 |
6 | 117289 |
MMP11
| Matrix metalloproteinase 11 (stromelysin 3) | AA045500 |
7 | 104973 |
SYNPO2
| Synaptopodin 2 | R31679 |
8 | 121066 |
DAPK3
| Death-associated protein kinase 3 | AA973730 |
9 | 128493 |
GMFG
| Glia maturation factor, gamma | AI311932 |
10 | 105533 |
ARF1
| ADP-ribosylation factor 1 | W45572 |
11 | 132031 | NY-REN-24 antigen | AA918005 | |
12 | 127881 |
NFIC
| Nuclear factor I/C (CCAAT-binding transcription factor) | T59427 |
13 | 120649 |
KCTD5
| Potassium channel tetramerisation domain containing 5 | AA521027 |
14 | 120934 |
EIF4G1
| Eukaryotic translation initiation factor 4 gamma, 1 | R37276 |
15 | 105453 |
PSAP
| Prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) | N72215 |
16 | 112695 |
SYNPO2
|
H. sapiens cDNA FLJ20767 fis, clone COL06986 | AA043349 |
17 | 101577 |
BMI1
| Murine leukaemia viral (bmi-1) oncogene homolog | AA478036 |
18 | 105479 |
BAT3
| HLA-B-associated transcript-3 | AA434416 |
19 | 123071 |
C9orf82
| Hypothetical protein FLJ13657 | AA135972 |
20 | 101638 |
ID4
| Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein | AA464856 |
21 | 115306 |
LRP16
| LRP16 protein | AA456318 |
22 | 118143 |
STX1B2
| ESTs, moderately similar to ST1B_HUMAN SYNTAXIN 1B [H. sapiens] | H41572 |
23 | 128106 |
DUSP6
| Dual specificity phosphatase 6 | AA455254 |
24 | 115676 |
RPS15A
| Ribosomal protein S15a | AA411682 |
25 | 108595 |
CCL19
| Small inducible cytokine subfamily A (Cys-Cys), member 19 | AA680186 |
26 | 126589 |
C6orf166
| Hypothetical protein FLJ10342 | AA984953 |
27 | 131540 |
PRPF31
| DKFZP566J153 protein | AI253017 |
28 | 109065 |
SYT5
| H39018 | |
29 | 128198 |
MMP11
| Matrix metalloproteinase 11 (stromelysin 3) | AA954935 |
30 | 109364 |
MYST2
| Histone acetyltransferase | H11938 |
31 | 106989 |
TNFSF13
| Tumour necrosis factor (ligand) superfamily, member 13 | AA443577 |
32 | 109798 | T82459 | ||
33 | 131890 |
CDH1
| Cadherin 1, type 1, E-cadherin (epithelial) | AI671174 |
34 | 111513 |
COG3
|
H. sapiens clone 25226 mRNA sequence | AA461166 |
35 | 108645 |
HMGCS2
| 3-Hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) | AA496149 |
36 | 101651 |
TRAP1
| Heat shock protein 75 | AA497020 |
37 | 105304 |
LRP16
| LRP16 protein | W52182 | AA284285 |
38 | 105363 |
ARL7
| ADP-ribosylation factor-like 7 | AA485683 |
39 | 127890 |
PTMS
| Parathymosin | AA458981 |
40 | 118682 |
NBS1
| Nijmegen breakage syndrome 1 (nibrin) | H98655 |
41 | 108997 |
PTTG1IP
| Pituitary tumour-transforming 1 interacting protein | AA156461 |
42 | 110281 |
HSPA1L
| Heat shock 70-kD protein-like 1 | H17513 |
43 | 125700 |
APC2
| Adenomatous polyposis coli like | AA976241 |
44 | 117139 |
ALDOB
| Aldolase B, fructose-bisphosphate | H72098 |
45 | 107595 |
SOX17
| SRY-box 17 | AA427400 | AI732705 |
46 | 107375 |
NUCKS
| Similar to rat nuclear ubiquitous casein kinase 2 | AA137266 |
47 | 109238 |
BSG
| Basigin (OK blood group) | AA436440 |
48 | 122821 |
NSE2
| ESTs | H30453 |
49 | 123689 |
LOC339123
| STIP1 homology and U-Box containing protein 1 | R54844 |
50 | 115953 |
LOC146542
| Human Chromosome 16 BAC clone CIT987SK-A-635H12 | AA455010 |
51 | 108960 |
GABRD
| H41122 | |
52 | 128222 |
GLUL
| Glutamate-ammonia ligase (glutamine synthase) | AI000103 |
53 | 100222 |
NFIX
| Nuclear factor I/X (CCAAT-binding transcription factor) | AA406269 |
54 | 105470 |
ISYNA1
| Myo-inositol 1-phosphate synthase A1 | AA454554 |
55 | 117998 |
RBM9
| RNA binding motif protein 9 | H03903 |
56 | 105404 |
GDF15
| Prostate differentiation factor | N26311 |
57 | 127811 |
TOB1
| Transducer of ERBB2, 1 | W96163 |
58 | 105524 |
RPS6KA4
| Ribosomal protein S6 kinase, 90-kD, polypeptide 4 | AA443601 |
59 | 109232 |
BCKDHA
| Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) | AA477298 |
60 | 115741 |
APPL
| Adaptor protein containing pH domain, PTB domain and leucine zipper motif | AA436158 |
61 | 100898 |
ELF3
| E74-like factor 3 (ets domain transcription factor, epithelial-specific) | AA434373 |
62 | 101067 |
MAP7
| Microtubule-associated protein 7 | R77252 | R77251 |
63 | 109306 |
AQP1
| Aquaporin 1 (channel-forming integral protein, 28 kD) | H24316 |
64 | 102326 |
CYC1
| Cytochrome c-1 | AA447774 |
65 | 108988 |
MALAT1
| Histone deacetylase 3 | H88540 |
66 | 102253 |
ACTG2
| Actin, gamma 2, smooth muscle, enteric | T60048 |
67 | 116834 |
GPC1
| Glypican 1 | AA455896 |
68 | 105497 |
HNRPK
| Heterogeneous nuclear ribonucleoprotein K | W85697 |
69 | 108372 |
LCP1
| Lymphocyte cytosolic protein 1 (L-plastin) | W73144 |
70 | 128634 |
PRCP
| Prolylcarboxypeptidase (angiotensinase C) | AI360366 |
71 | 106297 |
PHF17
| Hypothetical protein FLJ22479 | AA136664 |
72 | 101616 |
KRT19
| Keratin 19 | AA464250 |
73 | 128532 |
LTB
| Lymphotoxin beta (TNF superfamily, member 3) | AI351740 |
74 | 102385 |
F13A1
| Coagulation factor XIII, A1 polypeptide | AA449742 |
75 | 102673 |
WHSC1L1
| Wolf-Hirschhorn syndrome candidate 1-like 1 | T97900 |
76 | 109638 |
CXXC1
| CpG binding protein | T60082 |
77 | 109116 |
FBL
| Fibrillarin | AA663986 |
78 | 109425 |
TUBB
| Tubulin, beta polypeptide | AA427899 |
79 | 117500 | EST | AA621138 | |
80 | 100656 |
UBE2C
| Ubiquitin carrier protein E2-C | AA430504 |
Supervised classification of well-versus poorly differentiated DCIS
Rank | NKI ID | Symbol | Annotation | Accession no. |
---|---|---|---|---|
1 | 108691 |
ACK1
| Activated p21cdc42Hs kinase | AA427891 |
2 | 109246 |
BCL2
| B-cell CLL/lymphoma 2 | W63749 |
3 | 109268 |
ALDH3A2
| Aldehyde dehydrogenase 3 family, member A2 | AA633569 |
4 | 109236 |
BTD
| Biotinidase | R17765 |
5 | 108595 |
CCL19
| Small inducible cytokine subfamily A (Cys-Cys), member 19 | AA680186 |
6 | 100524 |
CELSR2
| Cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog | H39187 |
7 | 126868 |
TMC4
| DKFZP586J0619 protein | AA991211 |
8 | 100708 |
SLC39A6
| LIV-1 protein, oestrogen regulated | H29315 |
9 | 109170 |
C4A
| Complement component 4A | AA664406 |
10 | 109127 |
ESR1
| Oestrogen receptor 1 | AA291749 |
11 | 128702 | EST | AI313031 | |
12 | 121012 |
HSHIN1
| Hin-1 | AA902831 |
13 | 128095 |
PCSK6
| Paired basic amino acid cleaving system 4 | W85807 |
14 | 128052 |
ARHGEF7
| PAK-interacting exchange factor beta | AA452871 |
15 | 128493 |
GMFG
| Glia maturation factor, gamma | AI311932 |
16 | 123382 |
HIG1
| Likely ortholog of mouse hypoxia induced gene 1 | T74105 |
17 | 129689 |
C1orf21
| Chromosome 1 open reading frame 21 | AA406569 |
18 | 102289 |
ETFA
| Electron-transfer-flavoprotein, alpha polypeptide (glutaric aciduria II) | T57919 |
19 | 126124 |
FLJ20152
| Hypothetical protein | AA918685 |
20 | 127815 |
PLAT
| Plasminogen activator, tissue | R38933 |
21 | 101559 |
NPY1R
| Neuropeptide Y receptor Y1 | R43817 |
22 | 100260 |
MAL
| Mal, T-cell differentiation protein | AA227885 |
23 | 127969 |
CRYAA
| Crystallin, alpha A | H84722 |
24 | 128244 |
SERPINA3
| Serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 | AA704242 |
25 | 108649 | Human clone 23948 mRNA sequence | H15114 | |
26 | 106399 |
GRTP1
| Hypothetical protein FLJ22474 | N52651 |
27 | 123478 |
FLJ14712
| Hypothetical protein FLJ14712 | N79050 |
28 | 117207 |
EMP3
| Epithelial membrane protein 3 | W73810 |
29 | 111787 |
ZNF451
|
H. sapiens cDNA FLJ13010 fis, clone NT2RP3000542 | AA486412 |
30 | 109502 |
KITLG
|
H. sapiens cDNA: FLJ21592 fis, clone COL07036 | H11088 |
31 | 109315 |
UCP2
| Uncoupling protein 2 (mitochondrial, proton carrier) | H61243 |
32 | 118532 |
NUPL1
| PRO2463 protein | AA772502 |
33 | 100263 |
MYB
| V-myb avian myeloblastosis viral oncogene homolog | N49284 |
34 | 128249 |
CD3E
| CD3E antigen, epsilon polypeptide (TiT3 complex) | AA933862 |
35 | 131226 |
IL7R
| Interleukin 7 receptor | T65739 |
36 | 100104 |
SELL
| Selectin L (lymphocyte adhesion molecule 1) | H00662 |
37 | 108671 |
BCAT2
| Branched chain aminotransferase 2, mitochondrial | AA436410 |
38 | 116984 |
ATP5B
| ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide | AA708298 |
39 | 108376 |
LAMA3
| Laminin, alpha 3 (nicein [150 kD], kalinin [165 kD], BM600 [150 kD], epilegrin) | AA001432 |
40 | 104944 |
SLC7A2
| Solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 | R26163 |
41 | 100840 |
THOC1
| Nuclear matrix protein p84 | AA129297 |
42 | 100650 |
SHFM1
| Deleted in split-hand/split-foot 1 region | H85464 |
43 | 101429 |
SIAT1
| Sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase) | AA598652 |
Rank | NKI ID | Symbol | Annotation | Accession no. |
---|---|---|---|---|
1 | 111275 | EST | H20757 | |
2 | 109268 |
ALDH3A2
| Aldehyde dehydrogenase 3 family, member A2 | AA633569 |
3 | 109236 |
BTD
| Biotinidase | R17765 |
4 | 110384 |
KPNA2
| Karyopherin alpha 2 (RAG cohort 1, importin alpha 1) | AA676460 |
5 | 131448 |
PLEKHG1
| KIAA1209 protein | AI301815 |
6 | 108691 |
ACK1
| Activated p21cdc42Hs kinase | AA427891 |
7 | 107840 |
EPC1
| ESTs | AA120875 |
8 | 126868 |
TMC4
| DKFZP586J0619 protein | AA991211 |
9 | 106257 |
FLJ32499
|
H. sapiens cDNA FLJ12749 fis, clone NT2RP2001149 | W56590 |
10 | 128493 |
GMFG
| Glia maturation factor, gamma | AI311932 |
11 | 128702 | AI313031 | ||
12 | 129547 |
METAP2
| Methionine aminopeptidase; eIF-2-associated p67 | AA283030 |
13 | 111787 |
ZNF451
|
H. sapiens cDNA FLJ13010 fis, clone NT2RP3000542 | AA486412 |
14 | 103209 |
RBMS1
|
H. sapiens mRNA; cDNA DKFZp564H0764 (from clone DKFZp564H0764) | R62566 |
15 | 108595 |
CCL19
| Small inducible cytokine subfamily A (Cys-Cys), member 19 | AA680186 |
16 | 129267 | AA609203 | ||
17 | 109127 |
ESR1
| Oestrogen receptor 1 | AA291749 |
18 | 100263 |
MYB
| V-myb avian myeloblastosis viral oncogene homolog | N49284 |
19 | 100524 |
CELSR2
| Cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog | H39187 |
20 | 100260 |
MAL
| Mal, T-cell differentiation protein | AA227885 |
21 | 102995 |
PIGT
| CGI-06 protein | H82992 |
22 | 108649 | Human clone 23948 mRNA sequence | H15114 | |
23 | 109246 |
BCL2
| B-cell CLL/lymphoma 2 | W63749 |
24 | 100203 |
TNFAIP3
| Tumour necrosis factor, alpha-induced protein 3 | AA476272 |
25 | 107809 |
XBP1
| X-box binding protein 1 | W90128 |
26 | 102921 |
H. sapiens mRNA; cDNA DKFZp434D0818 (from clone DKFZp434D0818) | N95578 | |
27 | 108671 |
BCAT2
| Branched chain aminotransferase 2, mitochondrial | AA436410 |
28 | 101925 |
EZH2
| AA430744 | |
29 | 123382 |
HIG1
| Likely ortholog of mouse hypoxia induced gene 1 | T74105 |
30 | 131187 |
KPNA2
| Karyopherin alpha 2 (RAG cohort 1, importin alpha 1) | AA489087 |
31 | 111288 |
H. sapiens mRNA; cDNA DKFZp564C2063 (from clone DKFZp564C2063) | AA416628 | |
32 | 109170 |
C4A
| Complement component 4A | AA664406 |
33 | 108203 |
TEGT
| Testis enhanced gene transcript (BAX inhibitor 1) | AA629591 |
34 | 102639 |
EML2
| Microtubule-associated protein like echinoderm EMAP | R27580 |
35 | 131258 |
PSMA7
| Proteasome (prosome, macropain) subunit, alpha type, 7 | AI318565 |
36 | 123478 |
FLJ14712
| Hypothetical protein FLJ14712 | N79050 |
37 | 109415 |
FCGBP
| Fc fragment of IgG binding protein | R52030 |
38 | 127815 |
PLAT
| Plasminogen activator, tissue | R38933 |
39 | 115769 | ESTs | AA406313 | |
40 | 106220 |
GIMAP5
| Hypothetical protein FLJ11296 | AA150443 |
41 | 128641 |
PTTG1
| Pituitary tumour-transforming 1 | AI362866 |
42 | 105439 |
TGOLN2
| Trans-Golgi network protein (46-, 48-, 51-kD isoforms) | T81338 |
43 | 101362 |
ERBB2
| V-erb-b2 avian erythroblastic leukaemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) | AA446928 |
44 | 108387 |
IDH2
| Isocitrate dehydrogenase 2 (NADP+), mitochondrial | AA679907 |
45 | 100352 |
TGOLN2
| Trans-Golgi network protein (46-, 48-, 51-kD isoforms) | H82891 |
46 | 107941 |
PLAC8
| Hypothetical protein | AA150263 |
47 | 100104 |
SELL
| Selectin L (lymphocyte adhesion molecule 1) | H00662 |
48 | 110983 |
DLEU1
| Deleted in lymphocytic leukaemia, 1 | AA425755 |
49 | 108438 |
GRB7
| Growth factor receptor-bound protein 7 | H53703 |
50 | 107752 |
PAG
| Phosphoprotein associated with GEMs | N50114 |
51 | 128532 |
LTB
| Lymphotoxin beta (TNF superfamily, member 3) | AI351740 |
52 | 124620 |
ASTN2
| KIAA0634 protein | AA404602 |
53 | 102357 |
CHN1
| Chimerin (chimaerin) 1 | AA598668 |
54 | 109454 |
AKR7A2
| Aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) | T62865 |
55 | 108678 |
CASP10
| Caspase 10, apoptosis-related cysteine protease | H80712 |
56 | 131111 |
CUGBP2
| CUG triplet repeat, RNA-binding protein 2 | AA047257 |
57 | 123475 |
C9orf87
| Hypothetical protein FLJ10493 | N53432 |
58 | 105013 | EST | H61003 | |
59 | 100791 |
TDG
| Thymine-DNA glycosylase | AA496947 |
60 | 100528 |
BCL2L2
| BCL2-like 2 | AA454588 |
61 | 116312 |
FLJ14299
| Hypothetical protein FLJ14299 | AA453170 |
62 | 100700 |
TRIB2
| GS3955 protein | AA458653 |
63 | 102004 |
PIK3R1
| Phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) | R54050 |
64 | 104569 |
MYO1B
|
H. sapiens cDNA FLJ20153 fis, clone COL08656, highly similar to AJ001381 H. sapiens incomplete cDNA for a mutated allele | N95358 |
65 | 113907 |
SNRPB2
| Small nuclear ribonucleoprotein polypeptide B" | H00286 |
66 | 128683 |
WASL
| Wiskott-Aldrich syndrome-like | AI261600 |
67 | 123768 |
DUSP22
| Mitogen-activated protein kinase phosphatase x | H42417 |
68 | 105099 |
RET
| Ret proto-oncogene (multiple endocrine neoplasia MEN2A, MEN2B and medullary thyroid carcinoma 1, Hirschsprung disease) | H24956 |
69 | 116859 |
STMN1
| Leukaemia-associated phosphoprotein p18 (stathmin) | AA873060 |
70 | 111660 |
FLJ13710
| ESTs | AA120866 |
71 | 100112 |
SAA1
| Serum amyloid A1 | H25546 |
72 | 100840 |
THOC1
| Nuclear matrix protein p84 | AA129297 |
73 | 129239 | EST, Moderately similar to AF119917 63 PRO2831 [H. sapiens] | W95750 | |
74 | 115662 |
GIMAP4
| Hypothetical protein FLJ11110 | AA406363 |
75 | 109607 |
HTPAP
| ESTs | T48412 |
76 | 108692 |
EMP2
| Epithelial membrane protein 2 | T88721 |
77 | 105133 |
JUNB
| Jun B proto-oncogene | N94468 |
78 | 129959 |
LOC283352
| EST | AI023540 |