Background
Human bladder cancer is the most common urinary system malignancy in the world. According to global cancer statistics, approximately 74,000 cases of bladder cancer will be diagnosed in the USA in 2015, leading to an estimated 16,000 deaths [
1]. Bladder cancer consists of three histological and pathological types: urothelial carcinoma, squamous cell carcinoma and adenocarcinoma. Urothelial carcinoma, also known as transitional cell carcinoma (TCC), is the most common type, accounting for 90 % of all bladder cancer cases [
2,
3]. Clinically, studies have shown that non-muscle invasive bladder cancer (stages Ta – T1) accounts for approximately 70–80 % of all cases, with the remainder being characterized as muscle invasive bladder cancer (stages T2–T4). Furthermore, 10–30 % of non-muscle invasive bladder cancer (NMIBC) will progress to muscle invasive bladder cancer (MIBC) [
4,
5]. MIBC patients have a much worse outcome with regards to tumor recurrence and progression, with a 5-year survival rate of 25–60 % [
6‐
8]. Thus, additional noninvasive biomarkers for the prediction and diagnosis of bladder cancer are needed in the clinic.
Epigenetic changes are early and frequent events in cancer that play an important role in carcinogenesis. DNA methylation is the most common epigenetic alteration in human cancers [
9‐
11]. The detection of aberrantly methylated genes can be used as a diagnostic or prognostic biomarker for human cancers, especially when the aberrant methylation silences tumor suppressor genes [
12‐
14]. The
CDH13 gene, located on 16q24, encodes a protein that belongs to the cadherin family [
15].
CDH13, a tumor suppressor gene (TSG), is also called H-cadherin or T-cadherin and plays a pivotal role in cell–cell adhesion [
16]. The expression of
CDH13 in human tumor cells can inhibit their invasive potential and markedly reduce their proliferation [
17‐
19].
CDH13 promoter methylation has been reported in some human cancers including bladder cancer [
16].
However, the association between CDH13 promoter methylation and bladder cancer remains to be clarified. In this study, a meta-analysis was conducted to evaluate the effect of CDH13 methylation on the clinicopathological features of patients with bladder cancer.
Methods
Search strategy
A systemic literature search for studies published prior to November 16, 2015 was conducted in the PubMed, Embase, EBSCO, CKNI and Wanfang databases without any language restrictions. The following keywords and search terms were used: (CDH13 OR cadherin 13 OR H-cadherin OR T-cadherin) AND (bladder cancer OR bladder tumor OR bladder carcinoma OR bladder neoplasm) AND (methylation OR epigenetic silencing). The reference lists of the retrieved articles and reviews were then manually searched to identify potentially relevant studies.
Inclusion criteria
The eligible studies met the following criteria: 1) the patients were diagnosed with bladder cancer based on histopathology; 2) CDH13 methylation was evaluated in different types of samples, such as tissue, serum, plasma and urine; 3) regarding control samples by cystoscopy and histopathological confirmation, tissue samples belonged to normal tissues, while fluid samples such as serum, plasma or urine were from healthy individuals or patients with benign urological diseases; 4) the studies showed the associations between CDH13 methylation and clinicopathological parameters, including gender (male vs female), cancer tumor/node/metastasis (TNM) stage (T2/T4 vs Ta/T1), grade (grade 3 vs grade1/2) or tumor number (multiple vs single); 5) the methylation frequency of the CDH13 gene was sufficient for the case-control or cohort studies; 6) the studies were published in English or Chinese. The studies that were excluded did not meet our inclusion criteria. When the authors published more than one paper using the same sample data, either the most recent study or the study using the largest sample size was selected. The current meta-analysis was reported based on the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement.
The following pieces of information from the eligible studies were collected: first author surname, year of publication, ethnicity, histological type, types of samples, detection method, number of samples, clinicopathological parameters, gender, stage, grade, tumor number, frequency of CDH13 methylation, etc. As a control group, our meta-analysis used non-cancerous samples including non-cancerous diseases of the bladder and normal healthy tissue, according to each individual study in the original literature. Of these studies, a tumor stage of ≤ 1 was defined as early stage, a tumor stage of ≥ 2 was defined as advanced stage, a tumor grade of ≤ 2 was defined as low-grade, and a tumor grade of 3 was defined as high-grade. The final eligible studies were independently assessed by two reviewers for the current meta-analysis.
Data analysis
The analysis was conducted using STATA 12.0 (Stata Corporation) to evaluate the relationships between
CDH13 methylation and bladder cancer via the pooled odds ratio (OR) and the corresponding 95 % confidence interval (95 % CI). The frequency of
CDH13 methylation was analyzed according to various cancer characteristics. A statistical test for heterogeneity was performed based on the chi-square test and Q statistic [
20]. If substantial heterogeneity (I
2 ≥ 50 % or
p < 0.1) was observed, a random-effects model was used to calculate the parameters. Otherwise, a fixed effects model assuming a lack of heterogeneity was used [
21,
22]. Egger’s test was used to evaluate for possible publication bias [
23]. A
p value of less than 0.05 was considered statistically significant.
Discussion
DNA methylation in the blood, sputum, urine, feces, and other bodily fluids can be used as a non-invasive biomarker for the early detection of various cancers [
12,
33,
34]. Aberrant methylation of the
CDH13 gene has been reported in many cancers, including non-small cell lung cancer [
35], breast cancer [
36], gastric cancer [
37], and colorectal carcinoma [
38]. However, the potential of
CDH13 gene methylation to be a biomarker for bladder cancer has not yet been evaluated. The methylation rate of
CDH13 gene was relatively lower in non-tumor control samples, with a mean methylation frequency of 1.1 % in this study. The findings of the current study showed that
CDH13 promoter methylation was significantly higher in bladder cancer patients than in non-tumor control samples (OR = 21.71,
P < 0.001), suggesting that the methylation of
CDH13 may be involved in the development of bladder cancer. No significant heterogeneity was observed in cancer vs. controls (
p = 0.495 and I
2 = 0.0 %), indicating the reliability of our results. In addition, the result of a subgroup analysis based on sample type suggested that the
CDH13 methylation status was significant in both tissue and urine samples (OR = 53.94,
P < 0.001; OR = 7.71,
P < 0.001; respectively), indicating that the detection of
CDH13 methylation has the potential to be a non-invasive biomarker in the urine, which may aid in the early screening for and the diagnosis of bladder cancer. The OR value in Asians (OR = 35.18,
P < 0.001) was significantly higher than in Caucasians (OR = 8.86,
P < 0.001), which revealed that
CDH13 methylation may be a relatively more important risk factor among Asian populations.
In addition, we conducted meta-analyses to determine the correlations between
CDH13 methylation and clinicopathological characteristics. The results showed that the
CDH13 methylation status was not associated with gender (OR = 1.46, 95 % CI = 0.99–2.15,
P = 0.053). The levels of methylated
CDH13 were significantly higher in muscle invasive bladder cancer (stages T2–T4) than in non-muscle invasive bladder cancer (stages Ta – T1) (OR = 3.42,
P < 0.001).
CDH13 methylation status was significantly associated with high-grade (grade 3) bladder cancer (OR = 2.22,
P < 0.001). The levels of methylated
CDH13 were significantly higher in bladder cancer consisting of multiple tumors than in bladder cancer consisting of a single tumor (OR = 1.45,
P = 0.032). Patients with multiple tumors, high-grade bladder cancer, or muscle invasive bladder cancer are characterized by a high incidence of recurrence and progression and a poorer outcome [
39,
40]. Our findings indicated that
CDH13 promoter methylation was a very useful biomarker that can predict the recurrence of bladder cancer.
Some limitations of the current meta-analysis should be considered. First, the inclusion of articles published only in English and Chinese might lead to a selection bias. Second, the primary ethnic population of the patients in the current study was Asian, while only two studies involving Caucasians were involved in this meta-analysis. Other ethnicities, such as Africans, were limited. Third, due to the limitation of eligible studies in fluid samples, we did not further evaluate the diagnostic capacity of CDH13 promoter methylation for patients with non-muscle invasive bladder cancer. Thus, more studies based on urine and blood samples are very essential to evaluate whether CDH13 promoter methylation can become a noninvasive biomarker for the detection and diagnosis of non-muscle invasive bladder cancer in the future. Therefore, additional studies incorporating larger sample sizes are required to confirm our results in the future.
Acknowledgements
This research was supported by grants from the Ningbo Natural Science Foundation “2009A610115 and 2009A610143”.