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Archives of Orthopaedic and Trauma Surgery

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01.12.2008 | Basic Science

Collagen IX in weight-bearing areas of human articular cartilage in late stages of osteoarthritis

verfasst von: Sebastian Koelling, Jenny Kruegel, Michael Klinger, Wolfgang Schultz, Nicolai Miosge

Erschienen in: Archives of Orthopaedic and Trauma Surgery | Ausgabe 12/2008

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Abstract

Aim

To determine if the amounts of collagen IX mRNA and protein are higher in osteoarthritic cartilage from weight-bearing areas of the knee joint compared to non-weight bearing areas in patients with stage IV osteoarthritis (OA).

Methods

Normal and OA cartilage samples were obtained from 15 patients undergoing total knee replacement or necropsies. mRNA was measured by real time RT-PCR and proteins were detected by Western blot and localized at the light and ultrastructural level.

Results

Collagen IX was found throughout all cartilage layers in healthy and OA tissue. Cells deposited collagen IX in the pericellular and interterritorial matrix and a 66% higher amount of collagen IX was detected in the pericellular matrix of the weight-bearing areas adjacent to the main defect in comparison to the macroscopically intact areas. This is in line with a 3.72 times higher amount of the respective mRNA.

Conclusion

The increased levels of collagen IX protein and its mRNAs found in the weight-bearing areas adjacent to the main cartilage defect might reflect an attempt on the part of the diseased cartilage tissue to stabilize and protect the remaining matrix of late-stage osteoarthritic cartilage from further destruction.

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Titel: Collagen IX in weight-bearing areas of human articular cartilage in late stages of osteoarthritis
verfasst von: Sebastian Koelling
Jenny Kruegel
Michael Klinger
Wolfgang Schultz
Nicolai Miosge
Publikationsdatum: 01.12.2008
Verlag: Springer-Verlag
Erschienen in: Archives of Orthopaedic and Trauma Surgery / Ausgabe 12/2008
Print ISSN: 0936-8051
Elektronische ISSN: 1434-3916
DOI: https://doi.org/10.1007/s00402-008-0611-0

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