Background
In developed countries, paediatric limb deficiencies are primarily congenital, and result from various causes and phenotypes. Causal factors include environmental insults, such as owing to chemicals and pharmaceuticals, and chromosomal or genetic defects. Thalidomide, warfarin, valproic acid, and phenytoin are well known as causal drugs of limb deficiencies [1]. Some genetic factors associated with hypoplastic limb defects and split hand/split foot malformation have been identified [2]. The prevalence of congenital limb deficiencies is reportedly 4.91 per 10,000 live births in South America (1967–1992) [3], 5.5 per 10,000 total births in Alberta, Canada (1980–2012) [4], and 6.9 per 10,000 total births in Northern Netherlands (1991–2010) [5]. According to the International Clearinghouse for Birth Defects Monitoring Systems (ICBDMS), the prevalence of congenital limb deficiency in Japan is reportedly 3.81 per 10,000 total births (2007–2011) [6]. Although some breakdown has been reported (transverse: 0.29; pre axial: 0.90; post axial: 0.25; intercalary: 1.04; mixed: 0.90; unspecified: 0.43 per 10,000 total births), distinctions between upper or lower limbs, laterality, and other details (e.g., level of transverse deficiency) have not been demonstrated. In addition, it seems difficult to make precise diagnoses at birth in some patients. Regarding anomalies of the upper limbs in the Japanese population, Ogino et al. reported incidences and details such as affected side, associated anomalies, and family history [7]; however, these anomalies consisted mostly of deformities without limb deficiency or reduction such as trigger finger, polydactyly, camptodactyly and clasped thumb, and the situation for deficiency only was uncertain.
Congenital limb deficiency is a rare and intractable disease, and these patients require consecutive care from birth, through the stages of growth, and up to adulthood. However, in Japan, medical care and education systems have been inadequate for affected children. In considering therapeutic approaches, it is necessary to include both the function and appearance of the defect. Treatment approaches vary according to the type of deficiency. For example, children with congenital unilateral transverse upper limb deficiencies are often prescribed prostheses [8]. For radial deficiency, surgeries such as wrist centralization on the ulna and reconstruction of the thumb are the treatment choices [9]. In proximal focal femoral deficiency, surgeries including amputation or rotationplasty may be considered to optimise prosthetic fitting and function [10]. There is debate regarding which surgical approach to choose in certain situations. For example, for Jones type 1a tibial deficiency, one surgical approach is tibial centralization surgery (Brown’s procedure), to reconstruct the function of the knee-joint [11]. Another procedure is knee disarticulation to fit a prosthesis [12]. Regarding fibular dysplasia, one approach is foot reconstruction followed by limb-lengthening surgery, to preserve the affected limb; another is Syme’s amputation procedure, followed by a prosthetic fitting [13, 14].
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In Japan, nationwide epidemiological surveys have been performed in patients with various intractable diseases [15] such as familial Mediterranean fever [16], Moyamoya disease [17, 18], Churg–Strauss syndrome [19], adult Still’s syndrome [20], Sjögren’s syndrome [21], paediatric acquired demyelinating syndrome [22], and pancreatitis [23, 24]; however, congenital limb deficiency has not been studied in detail. To provide better evidence-based medical care, it is necessary to establish the concept of congenital limb deficiency and its grades of severity. In this study, we aimed to clarify the actual condition of congenital limb deficiency in Japan.
Methods
With the cooperation of the Research Committee on the Epidemiology of Intractable Diseases of Japan, we conducted a two-stage postal survey according to the Nationwide Epidemiological Survey Manual of Patients with Intractable Diseases (2nd edition 2006, Ministry of Health, Labour and Welfare of Japan).
This nationwide survey consisted of a first survey, in which the number of patients was estimated, and a second survey, in which basic clinical features were investigated. To achieve a high response rate, it was important to use a simple questionnaire. We confined the first survey to only a query of the number of patients who completed an initial visit. In the second survey, we requested the ages, symptoms, diagnostic details, associated anomalies, family histories of patients, and the previous hospitals and referral to other hospitals as the minimum of demographic and clinical information.
A list of all hospitals in Japan was obtained from the Research Committee on the Epidemiology of Intractable Diseases of Japan. The departments of orthopaedic surgery, paediatrics, and plastic surgery were extracted and subjected to stratified random sampling. Hospitals were categorized according to the institution type and number of hospital beds. Departments were randomly selected from these hospital categories; sampling rates were determined as approximately 5, 10, 20, 40, 80, and 100% for the stratum of general hospitals with 20–99 beds, 100–199 beds, 200–299 beds, 300–399 beds, 400–499 beds, and 500 or more beds, respectively. As the departments that most commonly treat patients with congenital limb deficiency, departments belonging to “the Japanese Association of Children’s Hospitals and Related Institutions” and “Rehabilitation Centers for Children with Physical Disabilities” were classified as “special stratum”, and all of these were selected for the survey. Other departments that could not be classified under any of the three departments above (i.e., hospitals in which medical care was provided by a specially associated group of multi-departments) were classified as “other special stratum,” and were selected for the survey.
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From this selection, 2283 departments (995 orthopaedic surgery departments, 812 paediatric departments, 472 plastic surgery departments), and four special departments were selected from a total of 7825 departments throughout Japan. In May 2016, we distributed the first survey querying the number of initial patient visits from January 2014 to December 2015. In this study, we defined congenital limb deficiency as partial or total absence of the limbs, proximal to the proximal interphalangeal joint of the fingers/lesser toes or interphalangeal joint of the thumb/great toe. We focused on deficiencies that cause relatively severe impairment and disability, for which treatment approaches have been discussed; therefore, we excluded deficits of the middle and distal phalanges. Polydactyly, syndactyly, and malformation or shortening of the limbs without deficit were also excluded from this study.
The targets of the second survey were departments that reported one or more initial patient visits in the first survey. In September 2016, we distributed the second survey querying patient details. In the second survey, patients who were ineligible, those who were not eligible because of their diagnosis or the time of their initial visit, and duplicate patients who were reported by two or more departments, were determined. Patients were identified as duplicated if their information (age, sex, diagnosis, and referral) was consistent among two or more departments. For duplicate patients, we included the data of their latest initial visit at the most recent, to obtain a more precise diagnosis and more detailed information. If a patient had visited more than one hospital during a short time, it is reasonable to think that the first institution saw the necessity for a more precise diagnosis or specialized medical treatment, such as a surgical procedure or prosthesis, and consulted a specialized department and that the most recent institution provided them with medical care. We identified the department for the date of the most recent initial visit as the actual institution that provided medical treatment at that time.
Statistics
Let n be the number of all departments, and ni be the number of departments with a number of targeted patients i (i = 0, 1, …). Let N be the number of responding departments and Ni be the number of responding departments with a number of patients i (i = 0, 1, …). N and Ni were obtained from the survey, and {Ni} followed a multi-hypergeometric distribution under the assumption of random response and under the condition that N is fixed.
Let a be the total number of patients; note that a = ∑i∗ni. The estimate of a was calculated as.
$$ a\hat{\mkern6mu} =\sum i\ast {N}_i\ast n/N. $$
The approximate 95% confidence intervals for a were calculated as.
$$ \left(a\hat{\mkern6mu} -1.96\ s,a\hat{\mkern6mu} +\kern0.37em 1.96\ s\right), $$
where s is an estimate of standard error of a^ and is given to be.
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\( s=\sqrt{\frac{\sum {i}^2\ast \frac{N_i}{N}-{\left(\sum i\ast \frac{N_i}{N}\right)}^2}{n-1}\kern0.28em \ast \kern0.28em {n}^3\kern0.28em \left(\frac{1}{N}-\frac{1}{n}\right)\kern0.28em }. \)
The number of patients in some strata was estimated using the above method. Let k be the number of strata, a1^, a2^, …, ak^ be the estimated numbers of patients in some strata, and s1, s2, …, sk be their standard errors. The approximate confidence intervals for the sum of the number of patients in the strata were calculated as.
$$ \left(a.\hat{\mkern6mu} -1.96\ s.,a.\hat{\mkern6mu} +\kern0.37em 1.96\ s.\right), $$
where a. = a1 ^ + a2 ^ + … + ak^ and \( s=\sqrt{{s_1}^2+{s_2}^2+\dots +{s_k}^2} \).
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Using the results of the first survey, the number of patients and 95% confidence intervals were estimated using the above procedure. Additionally, we corrected these using the ineligibility rate and duplication rate from the second survey. To obtain the final estimates and confidence intervals, we multiplied their values in the first survey by “1 − ineligibility rate” and “1 − duplication rate”, respectively.
Statistical analysis was performed using JMP® Pro 13.0.0 (SAS Institute Japan) and p < 0.05 was considered significant.
Results
Estimation of the number of patients
Table 1 summarizes the results of the first survey and shows the corrected number of patients with the results of the second survey. Of 2283 departments selected from the 7825 departments in Japan, 1767 responded to the first questionnaire concerning the number of patients with initial visits who had congenital limb deficiency: the response rate for the first survey was 77.4%. Details of the number of all, surveyed, and responding departments are shown in Additional file 1. Among them, 161 departments reported one or more patients with an initial visit. We then conducted the second survey among these 161 departments. Ninety-six departments responded to the second questionnaire concerning demographic and clinical information; the response rate for the second survey was 59.6%. Of these 96 departments, whereas a total number of 534 patients had an initial visit in the first survey, 491 patients with initial visits were recorded in the second survey. This difference of 43 patients was considered to represent ineligible patients and those excluded by the target departments. Some departments reported the reasons for exclusion as follows: intermixing of patients whose initial visit did not fall within the target period or did not fit the diagnosis (e.g., patients with acquired limb defects) in the first survey. Of the 491 patients, we excluded 38 ineligible patients and 42 duplicated patients; finally, 411 patients were eligible for inclusion in the analysis of demographic and clinical information. Of the 38 ineligible patients, 27 did not fall within the target period and 11 did not fit the diagnosis (4 patients had deficits of only the middle and/or distal phalanx, 4 had brachydactyly without deficits, 2 had constriction band without deficits, and 1 patient had polydactyly). The ineligibility rate was 15.2% and the duplication rate was 9.3%.
Table 1
Number of total, surveyed, and responded departments, and estimated number of patients
Strata | Total Number of Departments | Number of Surveyed Departments | Sampling rate (%) | Number of Responded Departments | Response Rate (%) | Estimated Number of Patients | 95% Confidence Interval | ||
|---|---|---|---|---|---|---|---|---|---|
First Survey | |||||||||
Orthopedic surgery | |||||||||
University hospital | 133 | 133 | 100.0 | 120 | 90.2 | 104 | 87 | – | 121 |
General hospital | |||||||||
≧500 beds | 201 | 201 | 100.0 | 155 | 77.1 | 25 | 16 | – | 33 |
400–499 beds | 213 | 170 | 79.8 | 135 | 79.4 | 0 | 0 | – | 0 |
300–399 beds | 343 | 137 | 39.9 | 104 | 75.9 | 7 | 0 | – | 14 |
200–299 beds | 391 | 79 | 20.2 | 59 | 74.7 | 0 | 0 | – | 0 |
100–199 beds | 1006 | 100 | 9.9 | 75 | 75.0 | 40 | 0 | – | 96 |
≦99 beds | 1950 | 96 | 4.9 | 64 | 66.7 | 0 | 0 | – | 0 |
Special stratum | 79 | 79 | 100.0 | 65 | 82.3 | 290 | 230 | – | 351 |
Subtotal | 4316 | 995 | 23.1 | 777 | 78.1 | 466 | 381 | – | 551 |
Pediatrics | |||||||||
University hospital | 125 | 125 | 100.0 | 106 | 84.8 | 24 | 18 | – | 29 |
General hospital | |||||||||
≧500 beds | 189 | 189 | 100.0 | 152 | 80.4 | 25 | 18 | – | 31 |
400–499 beds | 193 | 154 | 79.8 | 128 | 83.1 | 8 | 3 | – | 12 |
300–399 beds | 302 | 121 | 40.1 | 93 | 76.9 | 97 | 0 | – | 230 |
200–299 beds | 297 | 60 | 20.2 | 51 | 85.0 | 12 | 0 | – | 26 |
100–199 beds | 486 | 48 | 9.9 | 33 | 68.8 | 0 | 0 | – | 0 |
≦99 beds | 786 | 39 | 5.0 | 29 | 74.4 | 0 | 0 | – | 0 |
Special stratum | 76 | 76 | 100.0 | 50 | 65.8 | 15 | 6 | – | 25 |
Subtotal | 2454 | 812 | 33.1 | 642 | 79.1 | 180 | 47 | – | 314 |
Plastic surgery | |||||||||
University hospital | 89 | 89 | 100.0 | 68 | 76.4 | 47 | 35 | – | 60 |
General hospital | |||||||||
≧500 beds | 157 | 157 | 100.0 | 107 | 68.2 | 129 | 6 | – | 252 |
400–499 beds | 125 | 100 | 80.0 | 75 | 75.0 | 30 | 10 | – | 50 |
300–399 beds | 127 | 52 | 40.9 | 38 | 73.1 | 7 | 0 | – | 14 |
200–299 beds | 126 | 25 | 19.8 | 20 | 80.0 | 0 | 0 | – | 0 |
100–199 beds | 186 | 19 | 10.2 | 13 | 68.4 | 0 | 0 | – | 0 |
≦99 beds | 222 | 11 | 5.0 | 8 | 72.7 | 0 | 0 | – | 0 |
Special stratum | 19 | 19 | 100.0 | 15 | 78.9 | 46 | 28 | – | 63 |
Subtotal | 1051 | 472 | 44.9 | 344 | 72.9 | 259 | 132 | – | 385 |
Other special stratum | 4 | 4 | 100.0 | 4 | 100.0 | 179 | 179 | – | 179 |
Total | 7825 | 2283 | 29.2 | 1767 | 77.4 | 1084 | 881 | – | 1287 |
Total (per year) | 542 | 441 | – | 643 | |||||
Second Survey | |||||||||
Total (per year) a | 417 | 339 | – | 495 | |||||
Total (per 10,000 live births) b | 4.15 | 3.37 | – | 4.93 | |||||
We estimated that the number of initial visits of patients with congenital limb deficiency between 2014 and 2015 was 417 per year, (95% confidence interval 339–495). Considering that at the initial visit, most patients were aged 0 years, and that the number of live births in Japan was 1.004 million in 2014 and 1.006 in 2015, we estimated the prevalence of congenital limb deficiency with the assumption that the number of live births was 1.005 million (average of 2014 and 2015). The estimated prevalence was 4.15 (95% confidence interval 3.37–4.93) per 10,000 live births.
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Sex ratio
Among 411 patients reported in the second survey, there were 240 males and 171 females; the sex ratio was 1.40. Assuming an equal sex ratio (50% male, 50% female), a chi-square test yielded χ2 = 11.58, and p = 0.0007. This result suggests that males have a significantly higher prevalence of congenital limb deficiency than females.
Age
Table 2 summarizes the age of patients at their initial visit. Congenital limb deficiencies develop during the foetal stage, or before birth. The age reflects the patients’ access to medical care. It is necessary to consider that in cases of duplication, we included data for the date of the most recent initial visit to a department. A total 276 (67.2%) patients visited the hospital at age 0 years. The number of patients with initial visits decreased with advancing age.
Table 2
Patient age at the initial visit
Age | Number of Patients | % |
|---|---|---|
0 | 276 | 67.2 |
1 | 39 | 9.5 |
2 | 22 | 5.4 |
3 | 18 | 4.4 |
4 | 9 | 2.2 |
5 | 10 | 2.4 |
6 | 6 | 1.5 |
7 | 5 | 1.2 |
8 | 4 | 1.0 |
9 | 3 | 0.7 |
10 | 2 | 0.5 |
≥11 | 12 | 2.9 |
Unknown | 5 | 1.2 |
Total | 411 | 100 |
Affected limbs
Among 411 patients reported in the second survey, 275 (66.9%) had one affected limb, 83 (20.2%) had two, 23 (5.6%) had three, and 30 (7.3%) had four affected limbs. There were 275 patients (66.9%) with only the upper limbs affected, 75 (18.2%) with only lower limbs affected, and 61 (14.8%) with both upper and lower limbs affected.
The 411 patients had a total of 630 affected limbs among them. Four limbs had different classifications of deficiency (a total of 634 limb deficiencies) and all four affected limbs had coexisting proximal femoral focal deficiency and fibular dysplasia. Affected limbs were as follows: 218 (34.6%) were right upper limbs, 224 (35.6%) were left upper limbs, 105 (16.7%) were right lower limbs, and 83 (13.2%) were left lower limbs.
Classification of limb deficiency
Table 3 summarizes the classification of limb deficiency. For upper limbs deficiencies, transverse deficiency was the most prevalent (45.5%), followed by longitudinal deficiency(31.2%). For lower limbs deficiency, longitudinal deficiency was the most prevalent (37.0%), followed by transverse deficiency(31.8%). For longitudinal deficiency, whereas there was not much difference between tibial deficiency (33 limbs) and fibular deficiency (37 limbs) in the lower limbs, radial deficiency (110 limbs) was more prevalent than ulnar deficiency (27 limbs) in the upper limbs. For transverse deficiency, both upper limbs and lower limbs had similar tendencies in that distal deficiency comprised a large proportion and proximal deficiency was less frequent. The prevalence rates of intercalary, central, and other finger column deficiency were similar for both upper and lower limbs.
Table 3
Classification of congenital limb deficiency
Upper limbs | Lower Limbs | Total | ||||||
|---|---|---|---|---|---|---|---|---|
Number of deficiencies | % | Number of deficiencies | % | Number of deficiencies | % | |||
Longitudinal | Radial deficiency | 110 | 24.9 | Tibial deficiency | 33 | 17.2 | ||
Ulnar deficiency | 27 | 6.1 | Fibular deficiency | 37 | 19.3 | |||
Not classifiable | 1 | 0.2 | Not classifiable | 1 | 0.5 | |||
Subtotal | 138 | 31.2 | Subtotal | 71 | 37.0 | 209 | 33.0 | |
Transverse | Shoulder, Upper arm, Elbow | 16 | 3.6 | Hip, Thigh, Knee | 2 | 1.0 | ||
Forearm, Wrist | 41 | 9.3 | Lower leg, Ankle | 4 | 2.1 | |||
Hand | 141 | 31.9 | Foot | 54 | 28.1 | |||
Unknown | 3 | 0.7 | Unknown | 1 | 0.5 | |||
Subtotal | 201 | 45.5 | Subtotal | 61 | 31.8 | 262 | 41.3 | |
Intercalary | Phocomelia | 5 | 1.1 | Proximal femoral focal deficiency | 7 | 3.6 | ||
Subtotal | 5 | 1.1 | Subtotal | 7 | 3.6 | 12 | 1.9 | |
Central | Split hand | 75 | 17.0 | Split foot | 32 | 16.7 | ||
Subtotal | 75 | 17.0 | Subtotal | 32 | 16.7 | 107 | 16.9 | |
Other finger column deficiency | 23 | 5.2 | 21 | 10.9 | 44 | 6.9 | ||
Total | 442 | 100 | 192 | 100 | 634 | 100 | ||
Associated anomalies
Coexisting disorders with relatively high prevalence (over 1%), were cardiac anomaly, vertebral anomaly, renal anomaly, anal atresia, hypospadias, cleft lip/palate and developmental disorders (autism spectrum syndrome or attention deficit hyperactivity disorder) (10.2, 3.6, 2.4, 2.4, 2.2, 1.2, and 1.0% respectively). Eleven patients had chromosomal abnormality: 3 had 17p-related abnormalities (duplication, microduplication and deletion), 4 had trisomy 21, 1 had trisomy 18, 1 had 48 XXXX, and 2 patients unspecified chromosomal abnormalities. Seventeen patients were diagnosed with or suspected of having the following specific syndromes, associations or sequences: 5 patients had VATER association, 4 had Poland syndrome (1 patient concomitantly had Moebius syndrome), 2 had Cornelia de Lange syndrome, and 1 each, had Apert syndrome, CHARGE syndrome, FATCO syndrome, KOSAKI syndrome, Leri-Weill syndrome, Nager syndrome, Silver-Russell syndrome, facioauriculovertebral sequence, and autosomal dominant epidermolysis bullosa dystrophica (we excluded amniotic bands syndrome). Approximately 66.4% of patients had no coexisting disorders.
Family history
Seventeen (4.1%) patients had a family history of congenital limb deficiency, and 165 (40.1%) had no such history. The family histories of 229 (55.7%) patients were unknown. The extent of family history was left to the discretion of each department.
Table 4 shows the details of 17 patients with a positive family history of congenital limb deficiency. The breakdown of these 17 patients was as follows: 9 with split hand deformity; 3 with ulnar deficiency; 2 each with upper limb transverse deficiency, split foot, and tibial deficiency; and 1 each with radial deficiency, lower limb transverse deficiency, and other finger column deficiency. Four patients had multiple types of deficiencies: 1 each with split hand and foot, split hand and tibial deficiency, ulnar deficiency and split foot, and ulnar deficiency and tibial deficiency. Most columns in the questionnaire requiring information about the limb deficiency of family members were left blank; therefore, we could not identify whether the type of deficiencies were the same for these patients.
Table 4
Details of patients with a positive family history of congenital limb deficiency
Child | Deficiency of the limb | Associated anomaly | Relation of the relative | Limb anomaly of the relative |
|---|---|---|---|---|
1 | split hand of right upper limb | none | sibling | (not reported) |
2 | split hand of right upper limb | none | sibling | (not reported) |
3 | split hand of right upper limb | none | sibling | (not reported) |
4 | split hand of right upper limb | none | sibling | (not reported) |
5 | split hand of both upper limbs and split foot of both lower limbs | none | sibling | (not reported) |
6 | radial deficiency of left upper limb | Fanconi’s anemia | sibling | (not reported) |
7 | ulnar deficiency of right upper limb | none | sibling | (not reported) |
8 | transverse deficiency at the level of hand of right upper limb | none | sibling | (not reported) |
9 | split hand of right upper limb | none | mother | anomalies of radius and ulna |
10 | split hand of right upper limb | none | father | split hand |
11 | split hand of both upper limbs | none | father | split hand |
12 | split hand of left upper limb and tibial deficiency of both lower limbs | none | granduncle | split hand |
13 | split foot of right lower limb | none | someone other than sibling | (not reported) |
14 | ulnar deficiency of both upper limbs and split foot of both lower limbs | Leri-Weill syndrome | mother | Madelung deformity, and Leri-Weill syndrome |
15 | ulnar deficiency of both upper limbs and tibial deficiency of both lower limbs | none | mother and grandmother | upper limb anomaly |
16 | transverse deficiency at the level of hand of right upper limb | none | relative on the mother’s side | toe defect |
17 | transverse deficiency at the level of foot of both lower limbs | none | father and grandfather on the father’s side | anomaly of fifth finger |
Discussion
This is the first study providing the detailed epidemiology of congenital limb deficiency in Japan. We achieved a higher response rate (77.4%) for the first survey than the 34–74% of similar studies conducted in Japan [16‐24]. This may reflect an interest in limb deficiency by orthopaedic surgeons, paediatricians, and plastic surgeons. The estimated prevalence of congenital limb deficiency in Japan of the present study was similar to that of previous studies in other countries [3‐5].
Whereas the duplication rate in this study seemed reasonable, the ineligibility rate seemed somewhat high. Similar surveys have reported low duplication rates, and the ineligibility rates varied by disease (2.3–13.2%) [17, 18, 20, 21]. One possible reason for the high ineligibility rate in this study was the inconsistent responses by some departments regarding the number of patients who had completed an initial visit and the total number of patients. In similar surveys in Japan, the number of patients and the number of initial patient visits, or only the number of patients were usually requested. In this study, to achieve a high response rate, we only queried the number of initial patient visits, which might have confused respondents.
The prevalence value was also similar to that reported in Japan by ICBDMS [6]; however the classification of congenital limb deficiency was different, especially for intercalary deficiency. In this study, intercalary deficiency included phocomelia and proximal femoral focal deficiency. In the ICBDMS report, a definition of “intercalary” was not available and the “limb reduction defects” did not include “central reduction.” These differences in definitions may have influenced the results of our survey. Our results were more similar to those of a study in the Northern Netherlands that reported fewer number of intercalary reduction defects than transverse, longitudinal, and central reduction defects [5].
The sex ratio was 1.40 in this study, which is similar to a study in Alberta, Canada, including 434 male and 344 female patients [4].
For most patients, the initial visit to the most recent institution was made at age 0 years, and the number of initial patient visits decreased with advancing age. Most congenital limb deficiencies are noted at birth, even if they are not diagnosed in detail; however, therapeutic interventions are rarely required in the neonatal period. No radical surgeries or therapies are normally carried out. It is recommended that the first fitting of prostheses commence at about age 6–9 months in children with limb deficiencies [27]; however, prosthetic therapy often begins later in Japan. In particular, prostheses for children with congenital upper limb deficiencies have not been sufficiently prescribed [28]. In our study, we assumed that there were patients who were untreated or were only followed-up without therapy and that some of them visited a specialized hospital for surgeries or prosthetic therapy or sought a second opinion during childhood or later in life.
Upper limbs were more affected than lower limbs in the present study. Previous studies showed the same tendency [4, 5]. Commonly associated anomalies in our survey were cardiac anomaly, vertebral anomaly, renal anomaly, anal atresia, hypospadias, and cleft lip/palate. The study in the Northern Netherlands also indicated cardiovascular anomalies and urinary tract anomalies as anomalies that are frequently present with reduction defects [5]. The specific syndromes, associations, or sequences diagnosed or suspected in more than one patient were already known to be associated with congenital limb deficiency. Considering the data thus far, we feel certain about the validity of the present results.
Seventeen (4.1%) patients had a family history of congenital limb deficiency; however, the family history of 229 (55.7%) patients was unknown. Possible reasons for the substantial number of patients with unknown family histories include the following:
(1) The respondent may have completed the survey in detail, but the family history was truly unknown.
(2) Although respondents reported that there was no family history, the extent was insufficient (e.g., only parents were known to have no limb deficiency, but this information for grandparents was unknown), and the respondent thought it was inadequate to consider as not-existence.
(3) The respondents were too busy to complete the survey in detail.
If patients with “unknown” family histories were excluded, the prevalence of patients with a family history of congenital limb deficiency was 9.3%. It seems reasonable that the prevalence was estimated at 4–10% in this study. Most patients with a family history were those with split hand (a portion of these patients were associated with split foot or tibial deficiency). We speculate that split hand might have a stronger relationship with genetic factors than other limb deficiencies, such as transverse deficiency, of which constriction band is one cause. This is consistent with a study that identified the genetic factor associated with split hand/foot malformation and Gollop-Wolfgang complex (association with split hand and tibial deficiency) among Japanese patients [29]; this is also in keeping with the fact that most split hand/foot malformation, including Gollop-Wolfgang complex, present an autosomal dominant pattern of inheritance [2]. Another speculation is that patients with split hand procreate more frequently than those with other limb deficiencies; further study of this relationship is warranted.
The present survey was cross-sectional and involved analyses of demographics, and clinical information such as prevalence and classification. To promote the development of therapeutic approaches and social support of patients with congenital limb deficiency, further surveys based on the results of this study are required. A longitudinal survey is expected to reveal the actual conditions and challenges throughout life among these patients, such as education, employment, marriage, and reproduction.
Limitations
Although the response rate for the first survey was high (77.4%), the ineligibility rate (15.2%) in the second survey seemed somewhat high. The main reason for exclusion was that the time of the initial visit did not correspond with the target period. In addition, there were some patients who did not fit the appropriate definition of congenital limb deficiency used in this study (defined as partial or total absence of the limbs proximal to the proximal interphalangeal joint of fingers/lesser toes or interphalangeal joint of the thumb/great toe). The disease concept of congenital limb deficiency has not been established in Japan. The estimates number in the present study may have been overestimated or underestimated, or may have changed in accordance with the definition used for this disease.
Conclusions
We estimated the prevalence of congenital limb deficiency in Japan to be 4.15 (95% confidence interval 3.37–4.93) per 10,000 live births. Upper limbs were more affected than lower limbs. For upper limbs, transverse deficiency was the most prevalent, followed by longitudinal deficiency. For lower limbs, longitudinal deficiency was the most prevalent, followed by transverse deficiency. We also revealed information of the sex ratio, age at initial visit, associated anomalies, and family history. Although we believe that our results will contribute to establishing the disease concept and grades of severity of this disease, further surveys, especially longitudinal ones, will be needed to promote the development of therapeutic approaches and social support for patients with congenital limb deficiency.
Acknowledgements
We acknowledged the Ministry of Health, Labour, and Welfare for supporting this work. We are grateful to the occupational therapist, Satoko Noguchi for her contribution to clerical work.
Funding
This work was supported by research grants on Rare and Intractable Diseases of the Ministry of Health, Labour and Welfare of Japan [grant number H26-Nanchitou(Nan)-Ippan-089 and H27-Nanchitou(Nan)-Ippan-036], and by JSPS KAKENHI Grant Number 18K17890. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Availability of data and materials
All data generated or analysed during the first survey of this study are included in this published article and its supplementary information file.
The datasets generated and analysed during the second survey of current study are not publicly available. Those are concatenated datasets of sex, age, diagnosis, associated anomalies, and family history. Although these were anonymized, there is mathematical possibility of re-identification owing to the rarity of this disorder. However, these are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
This study was approved by the Ethics Committee of the Faculty of Medicine of the University of Tokyo (ethical approval number: 11269). In Japan, personal information should be protected by the law of “Act on the Protection of Personal Information.” The information we collected in this study was not regarded as the personal information and the need for consent was deemed unnecessary by the Ethics Committee of the Faculty of Medicine of the University of Tokyo.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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