Background
Methods
Plant material
Plant species/Family (Voucher specimen) | Traditional use | Sample used* (yield in %) | Index |
---|---|---|---|
Teucrium oliverianum/ Lamiaceae (15930) | 20.0 | SY-175 | |
Echium arabicum/ Boraginaceae (15931) | Antiplasmodial and antitrypanosomal activity[5] | 14.5 | SY-176 |
Haplophyllum tuberculatum/ Rutaceae (15932) | 19.6 | SY-177 | |
Senna italica/Caealpiniaceae (15933) | Diarrhea, stomachache, female infertility, tuberculosis, asthma[8] | 14.9 | SY-178 |
Pulicaria crispa/Asteraceae (15934) | Treat inflammation and an insect repellent[9] and is also used as an herbal tea | 6.7 | SY-179 |
Rhantarium epapposum/ Asteraceae (15935) | Skin infections and gastrointestinal disturbances and as an insecticide[10] | 3.1 | SY-180 |
Rumex vasicanus/ Polygonaceae (15936) | 13 | SY-181 | |
Ducrosia anethifolia/Aplaceae (15937) | Analgesic and pain reliever for headache, ackache, colic, and colds[14] | 26 | SY-182 |
Heliotropium ramosissimum/ Boraginaceae (15938) | Treatment of gout, rheumatism, and as anti-inflammatory and healing agents[15] | 2.58 | SY-183 |
Picris cyanocarpa/ Asteraceae (15939) | Treatment of indigestion, against intestinal nematodes and other parasites[16] | 19.5 | SY-184 |
Anthemis deserti/ Asteraceae (15940) | Herbal medicines, insecticides, and dyes, food additives, as well as an important source in aromatic and cosmetic industries[17] | 10.8 | SY-185 |
Cleome ambliocarpa/ Cleomaceae (15945) | Stomachics, rubefacients and in the treatment of scabies, rheumatic, fever, inflammation and a hypoglycemic agent,[18] | 14.9 | SY-186 |
Zilla spinosa/ Brassicaceae (15946) | Antioxidant, antifungal, hepatoprotective and antiviral activities[19] | 13.5 | SY-187 |
Ziziphus nummularia/ Rhamnaceae (15947) | Antibacterial, analgesic activities and anthelmintics[20] | 11.6 | SY-188 |
Neurada procumbens/ Neuradaceae (15949) | Diarrhea and dysentery; as well, it has been used as a tonic to ‘increase heart and respiration functions | 8.6 | SY-189 |
Trigonella hamosa/ Papilionaceae (15951) | A condiment and seasoning in food preparations and hypoglycemic | 22 | SY-190 |
Achillia fragrantissima/ Asteraceae (15952) | Respiratory diseases and gastrointestinal disturbances[21] | 6.2 | SY-191 |
Convolvulus prostates/ Convolvulaceae (15953) | Brain related disease, improve memory, skin disease[22] | 15.3 | SY-192 |
Cltrullus colocynthis/ Cucurbitaceae (15954) | Treat constipation, diabetes, edema, fever, jaundice, bacterial infections as well as cancer[23] | 14.2 | SY-193 |
Emex spinosa/ Polygonaceae (15955) | Purgative, diuretic, a remedy for stomach disorders, dyspepsia and colic[24] | 16.8 | SY-194 |
Rhazya strict/ Apocynaceae (15957) | Diabetes mellitus, fever, sore throat, inflammatory conditions and helminthiasis[25] | 22 | SY-195 |
Scrophularia hypericifolia/ Scrophulariaceae (15958) | 12.12 | SY-196 | |
Caylusea hexagyna/ Resedaceae (15959) | Anticancer (melanoma cell lines)[28] | 13.04 | SY-197 |
Artemisia monosperma/ Asteraceae (15960) | Antispasmodic, anthelmintic and anti-hypertensive[29] | 16.4 | SY-198 |
Ononis serrata/ Fabaceae (15925) | Antibiotic, antipyretic, anti-inflammatory, antifungal and antiseptic activities. Treatment of skin and rheumatic diseases as well as gout[30] | 5.5 | SY160 |
Achillea beiberstenii/ Asteraceae (15470) | Spasmolytic, choleretic, treatment of wounds and anti-inflammatory activities, make it as an important medicinal plant[31] | 10.4 | Ach-b |
Extraction
Chemicals
Cell cultures
Resazurin reduction assay
Determination of cell cycle distribution and apoptosis by flow cytometry
Caspase-Glo 3/7, caspase-Glo 8 and caspase-Glo 9 assay
Analysis of mitochondrial membrane potential (MMP)
Antibacterial assay
Bacterial strains and culture media
Bacterial susceptibility determinations
Statistical analysis
Results
Cytotoxicity
Tested samples | Samples code | Cell lines and IC50(μg/mL) | |
---|---|---|---|
CCRF-CEM | HL60 | ||
Haplophyllum tuberculatum
| SY177 | 9.94 | - |
Senna italica
| SY178 | 37.13 | - |
Pulicaria crispa
| SY179 | 1.81 | - |
Rhantarium epapposum
| SY180 | 31.9 | - |
Anthemis deserti
| SY185 | 30.08 | - |
Ziziphus nummularia
| SY188 | 16.6 | - |
Rhazya strict
| SY195 | 22.35 | - |
Artemisia monosperma
| SY198 | 19.64 | - |
Ononis serrata
| SY160 | 17.44 | 9.30 |
Achillea beiberstenii
| SY-200 | 18.11 | 5.06 |
Doxorubicin | 0.11 | 0.40 |
Tested samples | Sample code | Cell lines, IC50(μg/mL ) and degree of resistance* | |||||||
---|---|---|---|---|---|---|---|---|---|
MDA-MB-231/pcDNA | MDA-MB-231/BCRP | HCT116p53
+/+
| HCT116p53
-/-
| U87MG | U87MGΔEGFR
| HepG2 | AML 12 | ||
Haplophyllum tuberculatum
| SY177 | 11.92 | 26.65 (2.24) | 2.42 | 3.30 (1.36) | 15.03 | 27.67 (1.84) | 31.64 | - |
Pulicaria crispa
| SY179 | 14.6 | 8.37 (0.57) | 2.79 | 2.58 (0.92) | 4.31 | 27.46 (6.37) | 27.85 | - |
Anthemis deserti
| SY185 | - | - | 24.48 | 31.94 (1.30) | - | - | - | - |
Ziziphus nummularia
| SY188 | - | - | - | - | - | - | - | - |
Neurada procumbens
| SY189 | - | - | - | - | 3.01 | - | - | - |
Artemisia monosperma
| SY198 | - | 26.16 (<0.65) | 7.35 | 6.65 (0.90) | 25.25 | 24.95 (0.99) | - | - |
Ononis serrata
| SY160 | 19.28 | 25.09 (1.30) | 14.9 | 21.94 (1.47) | 28.58 | 31.09 (1.09) | - | - |
Achillea beiberstenii
| SY-200 | 10.48 | 22.32 (2.13) | 17.09 | 17.31 (1.01) | - | 19.24 (<0.48) | 27.28 | - |
Doxorubicin | 1.10 | 7.83 (7.12) | 1.41 | 4.06 (2.88) | 1.06 | 6.11 (5.76) | 1.41 | - |