Background
Method
Research design, context and participants
Phase of study | Participants’ job and number male(m) + female(f) | Assessment measure or target | |||
---|---|---|---|---|---|
Livestock breeder | Health educationist | Veterinarian | Experts from a vaccine providing institute | ||
Item generation | |||||
Interviews | 6 m | 4(1 m + 3f) | 4(3 m + 1 f) | 3 m | Identifying the factors influencing preventive behavior of livestock breeders |
Group discussion | 0 | 3f | 3 m | 3 m | Finalizing the first draft of the research questionnaire |
Assessment of face validity | |||||
Qualitative way | 15 m | 7(2 m + 5f) | Resolving ambiguity in meaning, wording, grammatical errors and allocation of the items | ||
Quantitative way | 15 m | 0 | 0 | 0 | Calculating IS of the items |
Assessment of content validity | |||||
Qualitative way | 0 | 7(2 m + 5f) | 7(5 m + 2f) | 3 m | Calculating CVR of the items |
Quantitative way | 0 | 7(2 m + 5f) | 7(5 m + 2f) | 3 m | Calculating CVI of the items |
Assessment of construct validity | |||||
EFA | 212 m | 0 | 0 | 0 | Calculating KMO of the questionnaire; factor loadings |
CFA | 220 m | 0 | 0 | 0 | Calculating fit indices of the structural behavioral model |
Reliability assessment | 42 m | 0 | 0 | 0 | Calculating Cronbach’s α, ICC,SEM,CR of the questionnaire |
Developing the first draft of the questionnaire
Assessment of face and content validity of the questionnaire
Assessment of construct validity of the questionnaire
Exploratory factor analysis (EFA)
Confirmatory factor analysis (CFA)
Assessment of reliability of the questionnaire
Results
Baseline characteristics of the participants
Characteristics | Total (n = 432) | Exploratory Factor analysis data (n = 212) | Confirmatory Factor analysis data(n = 220) | P-valuea |
---|---|---|---|---|
Number (%) | Number (%) | Number (%) | ||
Age: Mean ± SD) | 51.68 ± 16.40 | 51.43 ± 16.78 | 51.92 ± 16.05 | 0.76 |
Sex | ||||
Male | 432(100%) | 212(100%) | 220(100%) | |
Femaleb | 0(0%) | 0(0%) | 0(0%) | |
Animal type | 0.77 | |||
Cow | 53 (12.3%) | 25 (11.8%) | 28 (12.7%) | |
Sheep & Goat | 379 (87.7%) | 187 (88.2%) | 192 (87.3%) | |
Job | 0.91 | |||
self-employed | 16 (3.7%) | 6 (2.8%) | 10 (4.5%) | |
employee | 12 (2.8%) | 6 (2.8) | 6 (2.7%) | |
Farmer | 329 (76.1%) | 162 (76.4%) | 167 (75.9%) | |
Unemployed | 75 (17.4%) | 38 (18.0%) | 37 (16.9%) | |
Educational level | 0.95 | |||
Illiterate | 191 (44.2%) | 92 (43.4%) | 99 (45.0%) | |
Elementary | 14 (42.6%) | 93 (43.9%) | 91 (41.4%) | |
High school | 48 (11.1%) | 23 (10.8%) | 25 (11.4%) | |
Graduate diploma | 9 (2.1%) | 4 (1.9%) | 5 (2.3%) | |
Income | 0.64 | |||
Less than household expenses | 382 (88.4%) | 189 (89.2%) | 193 (87.7%) | |
Equal to household expenses | 50 (11.6%) | 23 (10.8%) | 27 (12.3%) | |
Prior brucellosis prevention | ||||
No | 412 (95.4%) | 202 (95.3%) | 210 (95.5%) | 0.93 |
Yes | 20 (4.6%) | 10 (4.7%) | 10 (4.5%) | |
Previous history of brucellosis in humans | 0.79 | |||
No | 256 (59.3%) | 127 (59.9%) | 129 (58.6%) | |
Yes | 176 (40.7%) | 85 (40.1%) | 91 (41.4%) | |
The number of family members (Range) | 7 (2–12) | 6 (3–11) | 7 (2–12) | 0.96 |
Results of the face validity of the BPQ
Construct or sub-construct | Item | Content Validity Ratio | Content Validity Index | Impact Score | The sourcea of the items (Literature/ interviews | |
---|---|---|---|---|---|---|
AD = direct awareness score | 1 | Can brucellosis be transmitted from an animal to a human? | 0.88 | 1 | 4.8 | L |
2 | Can brucellosis be transmitted from a sheep or a goat to a person? | 0.64 | 0.98 | 4.8 | L | |
3 | Can brucellosis be transmitted from a cow to a human? | 0.76 | 0.96 | 4.7 | L | |
4 | Is brucellosis transmitted from a person to another? | 0.76 | 0.86 | 4.4 | L | |
5 | Can brucellosis be transmitted from skin contact with an infected animal? | 0.88 | 1 | 4.2 | L | |
6 | Can brucellosis be transmitted by breathing into the infected stalls of livestock? | 0.64 | 0.84 | 4.7 | L | |
7 | Can brucellosis be transmitted through touching the aborted fetus and placenta of an infected animal? | 1 | 0.94 | 4.7 | L | |
8 | Can wearing gloves prevent brucellosis when you contact the uterine secretions of dead animals? | 0.64 | 0.80 | 4 | L | |
9 | Does brucellosis spread in the environment by the urine of animal? | 0.88 | 0.90 | 3.8 | L | |
10 | If your animal aborts its fetus, do you burn the aborted fetus? | 0.64 | 1 | 4.1 | L | |
11 | Does brucellosis spread in the environment by the’ fetus and placenta of animal? | 0.76 | 0.94 | 4.2 | I | |
12 | Does the brucellosis spread in the environment by wool? | 0.64 | 0.98 | 4.3 | L | |
13 | Does killing infected animals prevent the development of brucellosis? | 0.52 | 0.94 | 3.8 | L | |
`14 | Do you think that abortion place should be disinfected during the abortion? | 0.76 | 1 | 4.7 | L | |
15 | Do you think a dog can eat an aborted fetus? | 0.52 | 0.80 | 4.2 | I | |
16 | Do you think the aborted fetus needs to be buried? | 0.52 | 0.86 | 3.8 | I | |
17 | Is brucellosis a preventable disease? | 0.88 | 0.80 | 4.7 | L | |
18 | Should the place be disinfected after an abortion? | 0.76 | 0.80 | 4.2 | L | |
AID = Indirect awareness | 1 | Can brucellosis be transmitted through consuming infected milk and dairy products? | 0.64 | 0.90 | 4 | L |
2 | Can brucellosis be transmitted by semi-cooked meat? | 0.76 | 0.88 | 4.2 | L | |
3 | Can brucellosis be transmitted through breathing into the stalls of animals? | 0.88 | 0.92 | 4.1 | I | |
4 | Can washing the milking dishes prevent brucellosis? | 0.52 | 0.80 | 3.7 | I | |
5 | Can boiling milk prevent brucellosis? | 0.76 | 0.80 | 3.6 | L | |
6 | Can keeping cheese in salty water for two months before consumption prevent brucellosis? | 0.76 | 0.86 | 4.2 | L | |
7 | Does the use of a mask while cleaning the stable prevent Brucella disease from transmitting? | 0.64 | 0.90 | 3.8 | L | |
8 | Does brucellosis spread in the environment by the milk of infected livestock? | 0.88 | 0.92 | 4.7 | I | |
9 | Does brucellosis spread in the environment by the meat of infected livestock? | 0.64 | 0.94 | 4.6 | I | |
10 | Do you ask a veterinarian to help with animal abortion? | 0.52 | 0.98 | 4.1 | I | |
AV=Vaccine oriented behavior score | 1 | Can livestock vaccination prevent Malta fever among humans? | 0.52 | 0.92 | 4.7 | L |
2 | Can brucellosis be prevented by vaccinating livestock? | 0.76 | 0.80 | 4.2 | I | |
3 | Is animal vaccination expensive for you? | 0.88 | 0.90 | 4.3 | I | |
4 | Do you have access to livestock vaccination services? | 0.88 | 0.82 | 4.7 | I | |
5 | Does the veterinary organization offer timely vaccination services for your animals? | 0.64 | 0.96 | 4 | I | |
6 | Do veterinarians encourage you to vaccinate your livestock?? | 0.64 | 0.98 | 4.2 | I | |
7 | Do the health personnel encourage you to vaccinate your livestock? | 0.76 | 0.94 | 4.4 | L | |
8 | Do family members encourage you to vaccinate your livestock? | 0.64 | 1 | 4.1 | I | |
9 | In which season should brucellosis vaccine be injected? | 0.88 | 0.84 | 4.7 | L | |
10 | How often should the brucellosis vaccine be repeated? | 0.64 | 0.96 | 4.8 | I | |
11 | I would like to prevent the disease through vaccination since the veterinarian organization does not pay full compensation for the animals slaughtered due to brucellosis. | 0.52 | 0.82 | 4.2 | L | |
12 | At what age should livestock be vaccinated against brucellosis? | 1 | 0.98 | 4.8 | L | |
13 | Does brucellosis affect livestock growth after vaccination? | 0.88 | 0.80 | 4.2 | I | |
14 | Can livestock get brucellosis again after vaccination? | 0.52 | 0.82 | 4 | I | |
A = Attitude score | 1 | Abortion does not occur if animals are vaccinated. | 0.76 | 0.84 | 4.1 | L |
2 | Livestock breeders should ask a veterinarian to examine their livestock. | 0.88 | 0.98 | 3.9 | I | |
3 | I will not get brucellosis, if I touch Vaccinated animals’ milk, urine, placenta and fetus. | 0.76 | 0.94 | 4.6 | L | |
4 | As vaccination is time-consuming, I prefer getting brucellosis instead of vaccinating my animals. | 0.88 | 0.92 | 4.8 | L | |
5 | Animal vaccination is a very difficult process. | 0.52 | 0.96 | 4.2 | L | |
6 | If I get brucellosis, I will be unable to work for a long time. | 0.76 | 1 | 3.2 | L | |
7 | If I do not vaccinate the animals, my family and I will get brucellosis. | 0.88 | 0.82 | 4.7 | L | |
8 | If I vaccinate my livestock, people will not get brucellosis with consumption of my dairy products. | 0.76 | 0.86 | 4.8 | L | |
9 | I may still be in danger of getting brucellosis, even if I do preventative measures. | 0.88 | 0.98 | 4.2 | I | |
P = practice score | 1 | I keep the records and documents related to the medical history and vaccination of my animals in a proper place. | 1 | 0.94 | 4.8 | I |
2 | I have experienced abortion among my livestock. | 0.52 | 0.84 | 4.2 | L | |
3 | I always monitor the cold chain for the vaccines injected into my cattle. | 0.64 | 0.96 | 4.8 | L | |
4 | I vaccinate my animals at the proper and recommended time in a year or season. | 0.88 | 0.98 | 4.7 | L | |
5 | I have vaccinated my livestock against brucellosis in every year since many years ago. | 0.76 | 0.88 | 4.1 | L | |
6 | My livestock are in contact with other non-vaccinated livestock. | 0.64 | 0.82 | 3.8 | L | |
7 | During buying a new livestock, I am curious to know about its vaccination history. | 0.88 | 0.80 | 4.2 | I | |
8 | During buying a new animal, I ask a veterinarian to examine my animal. | 0.76 | 0.80 | 4 | I | |
Mean | 0.74 | 0.90 | 4.3 |
Results of the content validity of the BPQ
Results of the construct validity of the BPQ
Results of the exploratory factor analysis
Scale | χ2 | χ2/df | P | CFI | TLI | RMSEA (95% CI) | Eigenvalue | %variance | Cumulative % variance |
---|---|---|---|---|---|---|---|---|---|
Direct Awareness (AD) | 270.20 | 2.00 | < 0.001 | .940 | .932 | .069 (.057; .081) | 9.41 | 15.89 | 15.89 |
Indirect Awareness (AID) | 42.36 | 1.21 | 0.183 | .990 | .987 | .031 (.001; .061) | 4.93 | 14.81 | 30.70 |
Vaccine oriented Awareness (AV) | 235.87 | 3.06 | < 0.001 | .976 | .953 | .079 (.064; .093) | 5.65 | 12.73 | 43.43 |
Attitude | 44.40 | 1.64 | 0.019 | .991 | .988 | .055 (.023; .083) | 5.26 | 15.81 | 59.24 |
Practice | 91.6 | 4.58 | < 0.001 | .972 | .961 | .079 (.053; .106) | 4.67 | 15.78 | 75.02 |
Results of the confirmatory factor analysis
Direct Awareness (AD) | Indirect Awareness (AID) | Vaccine oriented Awareness (AV) | Attitude | Practice | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Items | loading | t-value | loading | t-value | loading | t-value | loading | t-value | loading | t-value |
AD1 | 0.925 | 27.870 | ||||||||
AD2 | 0.834 | 23.533 | ||||||||
AD3 | 0.702 | 12.375 | ||||||||
AD4 | 0.866 | 34.158 | ||||||||
AD5 | 0.917 | 35.040 | ||||||||
AD7 | 0.884 | 26.580 | ||||||||
AD8 | 0.222 | 3.568 | ||||||||
AD9 | 0.842 | 34.573 | ||||||||
AD11 | 0.799 | 16.714 | ||||||||
AD12 | 0.848 | 15.297 | ||||||||
AD14 | 0.565 | 11.379 | ||||||||
AD15 | 0.831 | 25.834 | ||||||||
AD16 | 0.758 | 11.966 | ||||||||
AD17 | 0.884 | 36.951 | ||||||||
AD18 | 0.578 | 9.986 | ||||||||
ID1 | 0.802 | 12.538 | ||||||||
ID2 | 0.705 | 16.687 | ||||||||
ID3 | 0.427 | 7.036 | ||||||||
ID4 | 0.587 | 12.442 | ||||||||
ID5 | 0.754 | 26.469 | ||||||||
ID6 | 0.852 | 17.374 | ||||||||
ID8 | 0.856 | 30.126 | ||||||||
ID9 | 0.793 | 16.429 | ||||||||
ID10 | 0.628 | 9.095 | ||||||||
AV1 | 0.599 | 11.516 | ||||||||
AV2 | 0.692 | 15.700 | ||||||||
AV3 | 0.205 | 3.062 | ||||||||
AV4 | 0.910 | 32.667 | ||||||||
AV5 | 0.718 | 20.175 | ||||||||
AV6 | 0.891 | 22.625 | ||||||||
AV7 | 0.832 | 23.891 | ||||||||
AV9 | 0.926 | 21.381 | ||||||||
AV10 | 0.356 | 6.135 | ||||||||
AV12 | −0.321 | −3.502 | ||||||||
AV13 | 0.554 | 10.857 | ||||||||
AV14 | 0.508 | 11.044 | ||||||||
A1 | 0.779 | 31.494 | ||||||||
A2 | 0.700 | 19.999 | ||||||||
A3 | 0.673 | 21.465 | ||||||||
A4 | 0.822 | 36.302 | ||||||||
A5 | 0.738 | 23.219 | ||||||||
A6 | 0.799 | 34.801 | ||||||||
A7 | 0.808 | 35.827 | ||||||||
A8 | 0.802 | 34.834 | ||||||||
A9 | 0.427 | 9.326 | ||||||||
P1 | 0.788 | 29.746 | ||||||||
P2 | 0.653 | 19.495 | ||||||||
P3 | 0.618 | 20.255 | ||||||||
P4 | 0.270 | 5.403 | ||||||||
P5 | 0.912 | 57.672 | ||||||||
P6 | 0.924 | 62.573 | ||||||||
P7 | 0.693 | 21.539 | ||||||||
P8 | 0.830 | 32.831 |
Scale | Number of Questions in scale | Mean ± SD | Range |
---|---|---|---|
Awareness in all | 36 | 14.71 ± 5.79 | 1–31 |
Direct Awareness (AD) | 15 | 3.36 ± 3.60 | 0–15 |
Indirect Awareness (AID) | 9 | 6.04 ± 2.50 | 0–9 |
Vaccine oriented awareness (AV) | 12 | 5.30 ± 2.87 | 0–11 |
Attitude | 9 | 25.46 ± 7.26 | 11–45 |
Practice | 8 | 17.42 ± 7.90 | 8–39 |
Results of the reliability of the questionnaire
Construct or sub = construct | Intra-class Correlation Coefficient ICC (95% CI) | Cronbach’s Alpha (α) | standard error of measurement (SEM) | Composite Reliability (CR) |
---|---|---|---|---|
Awareness in all | 0.958(0.920–0.978) | 0.865 | 5.448 | 0.974 |
Direct Awareness (AD) | 0.957(0.919–0.977) | 0.863 | 7.591 | 0.951 |
Indirect Awareness (AID) | 0.947(0.900–0.972) | 0.831 | 10.446 | 0.908 |
Vaccine oriented awareness (AV) | 0.885(0.783–0.939) | 0.718 | 11.330 | 0.895 |
Attitude | 0.896(0.803–0.945) | 0.833 | 8.231 | 0.912 |
Practice | 0.927(0.863–0.962) | 0.825 | 10.477 | 0.896 |