Background
Methods
Normal plasma (NP)
Heparin neutralisers
Lupus Ratio tests
APTT-based Lupus Ratio in test plasma heparinised in vitro
RVVT-based Lupus Ratio
Test plasma from heparinised patients
Coagulation instruments
Results
Choice of heparin neutraliser
APTT, cephalin 1/100 | APTT, cephalin 1/3200 | |||
---|---|---|---|---|
mean, seconds | % change | mean, seconds | % change | |
without heparin neutraliser | 30.4 | -- | 58.7 | -- |
Poly-L-Arginine 3.96 μg/ml | 33.8 | 11.2 | 55.8 | -4.94 |
Poly-L-Lysine 3.96 μg/ml | 29.7 | -2.4 | 79.8 | 36.06 |
Polybrene 3.96 μg/ml | 34.1 | 12.1 | 72.7 | 23.96 |
Protamine 3.96 μg/ml | 35.7 | 17.5 | 64.4 | 9.80 |
Heparinisation in vitro
a) Effect on APTT and the APTT-based LR
APTT-based Lupus Ratio median (range) | ||
---|---|---|
LA-positive patients n = 12 | LA-negative patients n = 8 | |
Lupus Ratio | 1.29 (1.07 to 2.24) | 0.99 (0.84 to 1.03) |
Lupus ratio with polybrene | 1.20 (1.01 to 2.19) | 0.94 (0.82 to 1.00) |
% change | -5.50 (-10.85 to +1.77) | -1.06 (-13.29 to +2.92) |
Lupus ratio with heparin and polybrene | 1.26 (1.04 to 2.22) | 0.98 (0.85 to 1.00) |
% change | -3.52 (-6.80 to --1.10) | -1.49 (-3.09 to +1.19) |
b) The effect on the RVVT and the RVVT-based LR
Heparin concentration | Polybrene concentration | Mean clotting time with cephalin 1/200 | Mean clotting time with cephalin 1/10 000 | Lupus Ratio |
---|---|---|---|---|
0.0 U/ml | 0.00 μg/ml | 29.8 seconds | 46.3 seconds | ------ |
0.0 U/ml | 3.96 μg/ml | 30.4 seconds | 48.8 seconds | 1.03 (neg.) |
0.5 U/ml | 3.96 μg/ml | 29.8 seconds | 46.9 seconds | 1.01 (neg.) |
0.5 U/ml | 0.00 μg/ml | 38.1 seconds | 75.4 seconds | 1.27 (false pos.) |
RVVT-based Lupus Ratio median (range) | ||
---|---|---|
LA-positive patients n = 12 | LA-negative patients n = 13 | |
Lupus Ratio | 1.33 (1.14 to 1.63) | 0.99 (0.88 to 1.06) |
Lupus ratio with polybrene | 1.36 (1.20 to 1.69) | 1.03 (0.92 to 1.11) |
% change | 2.33 (-1.52 to +10.16) | 2.94 (-1.02 to +9.57) |
Lupus ratio with heparin and polybrene | 1.32 (1.16 to 1.52) | 1.01 (0.87 to 1.08) |
% change | -2.51 (-9.40 to +6.78) | 1.09 (-3.03 to +5.21) |
Heparin treated patients
Patient | Anti-Xa activity after heparin i.v. | LR before heparin | LR before heparin, polybrene added | Change % | LR after heparin i.v. polybrene added | Change % |
---|---|---|---|---|---|---|
1 | 1.26 | 1.79 (+++) | 1.87 (+++) | 4.47 | 1.72 (+++) | -3.91 |
2 | 0.50 | 1.21 (+) | 1.23 (+) | 1.65 | 1.22 (+) | 0.83 |
3 | 0.65 | 1.76 (+++) | 1.79 (+++) | 1.70 | 1.81 (+++) | 2.84 |
4 | 1.12 | 2.02 (+++) | 1.96 (+++) | -2.97 | 1.92 (+++) | -4.95 |
5 | 1.15 | 1.05 (+) | 1.05 (+) | 0.00 | 1.08 (+) | 2.86 |
6 | 1.20 | 1.40 (++) | 1.32 (++) | -5.71 | 1.32 (++) | -5.71 |
7 | 1.23 | 1.15 (+) | 1.17 (+) | 1.74 | 1.19 (+) | 3.48 |
8 | 1.11 | 1.54 (+++) | 1.64 (+++) | 6.49 | 1.66 (+++) | 7.79 |
9 | 1.37 | 1.30 (++) | 1.34 (++) | 3.08 | 1.21 (+) | -6.92 |
10 | 0.72 | 1.33 (++) | 1.34 (++) | 0.75 | 1.31 (++) | -1.50 |
11 | 1.05 | 2.02 (+++) | 1.92 (+++) | -4.95 | 1.85 (+++) | -8.42 |
Median | 1.40 | 1.34 | 1.65 | 1.32 | -1.50 | |
Range | 1.05 to 2.02 | 1.05 to 1.96 | -5.71 to +6.49 | 1.08 to 1.92 | -8.42 to +7.79 |