The authors declare they have no competing interests.
JD completed all experiments, analyzed all data, and wrote the manuscript. NBK and QF conceived/designed the study, and reviewed/edited the manuscript. KDE was the collaborative oncologist for the study who helped with sample collection, and with review/editing of the paper. HJ assisted in sample collection. All authors read and approved the final manuscript.
Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples.
In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.
Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18).
Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.
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- Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
Keith D. Eaton
Nancy B. Kiviat
- BioMed Central
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