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01.12.2015 | Research article | Ausgabe 1/2015 Open Access

BMC Oral Health 1/2015

Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients

Zeitschrift:
BMC Oral Health > Ausgabe 1/2015
Autoren:
Juliet Dang, Qinghua Feng, Keith D. Eaton, Hona Jang, Nancy B. Kiviat
Wichtige Hinweise

Competing interests

The authors declare they have no competing interests.

Authors’ contributions

JD completed all experiments, analyzed all data, and wrote the manuscript. NBK and QF conceived/designed the study, and reviewed/edited the manuscript. KDE was the collaborative oncologist for the study who helped with sample collection, and with review/editing of the paper. HJ assisted in sample collection. All authors read and approved the final manuscript.

Abstract

Background

Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples.

Methods

In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.

Results

Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18).

Conclusion

Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.
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